In vivo regulation of murine hair growth: Insights from grafting defined cell populations onto nude mice

High-throughput reconstitution of epithelial–mesenchymal interaction in folliculoid microtissues by biomaterial-facilitated self-assembly of dissociated heterotypic adult cells

“…It has been shown that, compared with newborn epidermal keratinocytes, adult keratinocytes do not respond well to inductive signals from cultured DP cells to generate new HFs [19,49,50]. In the control group when disperse DP cells and keratinocytes are used, formation of new HFs is not observed (Fig.…”

Functional Characterization of Melanocyte Stem Cells in Hair Follicles

“…The HF reconstruction assay was performed as described (Lichti et al, 1993;Kishimoto et al, 1999;Claudinot et al, 2005;Yonetani et al, 2008), using W/W v mice (SLC, Shizuoka, Japan) as recipients of dermis and epidermis. Female mice carrying 12.5-to 13.5-day embryos were injected in the tail vein with 2 mg ACK2 antibody.…”

Dermal papilla cells induce keratinocyte tubulogenesis in culture

“…To facilitate studies into the mechanisms involved in the regulation of hair growth DP cell lines that retained specialized properties were established. Lichti et al (1993) produced adenovirus E1A-immortalized rat vibrissa DP cells that showed significant hair-inducing ability when combined with hair follicle buds in grafts. Yet there is no reliable model of epidermal morphogenesis in vitro using DP cells.…”