In vivo release of endogenous catecholamines, histamine and GABA in the hypothalamus of Wistar Kyoto and spontaneously hypertensive rats

Tuomisto, Leena; Yamatodani, Atsushi; Dietl, H.; Waldmann, U.; Philippu, A.

doi:10.1007/bf00497660

Cited by 46 publications

(3 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we compared the same specified site in the brain between SHRSP and WKY rats and found that GABA levels were lower in SHRSP than in WKY rats. This result was consistent with previous studies [17].…”

Section: Discussionsupporting

confidence: 95%

“…Next, we conducted a comparison between different animal models developed for specific diseases by comparing the GABA distribution in rat brain from SHRSP and WKY. It has been reported that the GABA level in the hypothalamus is significantly lower in the SHRSP brain than in WKY [17]; therefore, we looked for similar traits in our data. Furthermore, we comprehensively evaluated a range of amino acids and amine distribution with a normalization method.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Use of mTRAQ derivatization reagents on tissues for imaging neurotransmitters by MALDI imaging mass spectrometry: the triple spray method

Ito

Hiramoto

2019

Anal Bioanal Chem

View full text Add to dashboard Cite

During drug development, matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry is used for visually elucidating the distribution of substances such as biomarkers, candidate compounds, and metabolites in the tissues. However, it is difficult to make relative comparisons between tissue sections and there are still many challenges. Here, we report a new method of “triple spray” for the comparison of analyte distribution in multiple tissue slices. This method targets amino acids and amines, and it incorporates the application of the internal standard in the on-tissue derivatization step. With further development, it has the potential to alleviate problems caused by the matrix effect. Initially, we measured three serial sections of rat brain to verify the efficacy of this method. In the hypothalamus, where gamma-aminobutyric acid (GABA) is known to be present in high concentration, the GABA levels of the three serial section showed little variation (CV = 1.62%). Subsequently, we compared the GABA level in the brain between stroke-prone spontaneous hypertensive rats (SHRSP) and Wistar-Kyoto (WKY) rats with three individuals each. It showed significant differences between these models at the pre-selected region of interest (p < 0.05). Our results show that the triple spray allows for relative comparison among multiple tissue slices with high reproducibility. Graphical abstract

show abstract

Section: Discussionsupporting

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Use of mTRAQ derivatization reagents on tissues for imaging neurotransmitters by MALDI imaging mass spectrometry: the triple spray method

Ito

Hiramoto

2019

Anal Bioanal Chem

View full text Add to dashboard Cite

show abstract

“…The precise hypothalamic site involved remains to be defined. Although GABA release is not significantly different in the posterodorsal hypothalamus of Wistar-Kyoto (WKY) and SH rats (38), microinjection of muscimol directly into the DMH (then described as posterior hypothalamic area) induced a greater depressor response in SH rats than in normotensive rats (42). However, the injection of BMI into this area of the hypothalamus elicits similar increases in blood pressure and heart rate in SH and WKY rats (42).…”

Section: Discussionmentioning

confidence: 99%

Reduced GABA inhibition of sympathetic function in renal-wrapped hypertensive rats

Martin

Haywood

1998

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

Animals with bilateral cannulas in the paraventricular nucleus were made hypertensive by a one-kidney, figure eight renal wrap procedure or sham operated. Femoral artery and vein catheters were inserted for arterial pressure measurement and plasma catecholamine determination. After recovery and 4 days after hypertension surgery, bicuculline methiodide or muscimol was microinjected into the paraventricular nucleus. In some rats, nitroprusside was infused intravenously to reflexly stimulate the sympathetic nervous system. In control rats, bicuculline increased blood pressure, heart rate, and plasma norepinephrine and epinephrine concentrations. In contrast, in hypertensive rats blood pressure did not change while the heart rate response was maintained. Plasma norepinephrine and epinephrine responses were reduced 75 and 68%, respectively. Muscimol injections decreased arterial pressure in the hypertensive rats. Heart rate responses to nitroprusside were similar in the two groups of rats, while the plasma catecholamine responses were attenuated in the hypertensive animals. These data suggest that GABA function in the paraventricular nucleus is reduced in renal wrap hypertension.

show abstract

Histaminergic Transmission in the Brain

Schwartz

Garbarg

Pollard

1986

Comprehensive Physiology

View full text Add to dashboard Cite

In vivo release of endogenous catecholamines, histamine and GABA in the hypothalamus of Wistar Kyoto and spontaneously hypertensive rats

Cited by 46 publications

References 33 publications

Use of mTRAQ derivatization reagents on tissues for imaging neurotransmitters by MALDI imaging mass spectrometry: the triple spray method

Use of mTRAQ derivatization reagents on tissues for imaging neurotransmitters by MALDI imaging mass spectrometry: the triple spray method

Reduced GABA inhibition of sympathetic function in renal-wrapped hypertensive rats

Histaminergic Transmission in the Brain

Contact Info

Product

Resources

About