In Vivo Selection of Intermediately- and Highly- Malignant Variants of Triple-negative Breast Cancer in Orthotopic Nude Mouse Models

Abstract

Abstract. Aim: Triple-negative breast cancer (TNBC), defined by the absence of receptors for estrogen, progesterone and human epithelial receptor 2 (HER2), is a recalcitrant disease in need of effective therapy. We previously isolated very-highlyTriple-negative breast cancer (TNBC), defined by the absence of receptors for estrogen, progesterone and human epithelial receptor 2 (HER2), is a recalcitrant disease in need of effective therapy (1, 2).We previously developed a highly-invasive, TNBC variant using serial orthotopic implantation of MDA-MB-231 human breast cancer expressing red fluorescent protein (MDA-MB-231-RFP) in nude mice (3). MDA-MB-231-RFP was implanted orthotopically into the right lower mammary gland. After the orthotopic tumors grew, they were re-6273

tRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors

“…The changes of breast cancer cells in vitro have been replicated using direct orthotopic or heterotopic injection of cells into the mouse [15][16][17][18][19]. To investigate the impact of QTRT1 knockout on tumor outgrowth, the xenograft nude mouse model (n = 10 mice per group) was established by subcutaneous bilateral injection of QTRT1-KO or WT breast cancer cells.…”

Podoplanin expression is a prognostic biomarker but may be dispensable for the malignancy of glioblastoma

“…Serial transplantations of tumor material resulting in increased tumor growth rate and enhanced invasion are frequently used to model this evolution of aggressive tumor cells. 22,23 Thus, this experimental approach was used conducting 3 serial transplantations of the 3 human primary glioblastoma tumors GBMF2, GBMF3, and GBMF10 into immunocompromised mice. Indeed, with every stage of transplantation we observed a reduced survival of the recipients, indicative for increased aggressiveness of the tumor ( Figure 1B).…”

PIK3CA mutation affects the proliferation of colorectal cancer cells through the PI3K-MEK/PDK1-GPT2 pathway

“…Xenograft studies. A total of 3x10 6 HCT116 cells, 3x10 6 MUT cells in a volume of 0.1 ml or 1x10 7 MUT cells (26,27) in a volume of 0.2 ml serum-free medium were injected subcutaneously into 4-week athymic nude mice near the right hind leg. Once tumors reached an average size of 200 mm³, nude mice Statistical analysis.…”