In vivo self-bio-imaging of tumors through in situ biosynthesized fluorescent gold nanoclusters

Wang, Jianling; Zhang, Gen; Li, Qiwei; Jiang, Hui; Liu, Chongyang; Amatore, Christian; Wang, Xuemei

doi:10.1038/srep01157

Cited by 179 publications

(198 citation statements)

References 46 publications

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“…8(a, b, c, d)). This result confirms that metal nanoparticles can able to penetrate in A549 cells, and can be certainly detected using fluorescence microscope [35]. Moreover, in vitro cell image of the silver 12 nanoparticles in A549 cells are well interconnected with our results of spectrofluorometry.…”

Section: Fluorescence Assay and In Vitro Cellular Imagingsupporting

confidence: 73%

Facile synthesis of Curcuma longa tuber powder engineered metal nanoparticles for bioimaging applications

Sankar

Rahman

Varunkumar

et al. 2017

Journal of Molecular Structure

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Section: Fluorescence Assay and In Vitro Cellular Imagingsupporting

confidence: 73%

Facile synthesis of Curcuma longa tuber powder engineered metal nanoparticles for bioimaging applications

Sankar

Rahman

Varunkumar

et al. 2017

Journal of Molecular Structure

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“…Protein stabilized noble metal nanoclusters (P:NC) present an exciting option as an intracellular fluorescent marker (1)(2)(3)(4)(5)(6)(7)(8). P:NCs are small clusters of atoms ranging from 5 to 25 atoms which are comprised of an Au 0 core surrounded by a shell of Au + atoms that are bound to the stabilizing protein (9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

In situSynthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

West¹,

Schaeublin

Griep³

et al. 2016

ACS Appl. Mater. Interfaces

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To date, the directed in situ synthesis of fluorescent gold nanoclusters (AuNCs) has only been demonstrated in cancerous cells, with the theorized synthesis mechanism prohibiting AuNC formation in nontumorigenic cell lines. This limitation hinders potential biostabilized AuNC-based technology in healthy cells involving both chemical and mechanical analysis, such as the direct sensing of protein function and the elucidation of local mechanical environments. Thus, new synthesis strategies are required to expand the application space of AuNCs beyond cancer-focused cellular studies. In this contribution, we have developed the methodology and demonstrated the direct in situ synthesis of AuNCs in the nontumorigenic neuronal microglial line, C8B4. The as-synthesized AuNCs form in situ and are stabilized by cellular proteins. The clusters exhibit bright green fluorescence and demonstrate low (<10%) toxicity. Interestingly, elevated ROS levels were not required for the in situ formation of AuNCs, although intracellular reductants such as glutamate were required for the synthesis of AuNCs in C8B4 cells. To our knowledge, this is the first-ever demonstration of AuNC synthesis in nontumorigenic cells and, as such, it considerably expands the application space of biostabilized fluorescent AuNCs.

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“…In particular in vivo selective imaging of specific cells using AuNCs has shown much promise [6][7][8] . AuNCs are small in size, consisting of roughly 25 atoms in size and are less than 2nm in diameter, comparing with gold nanoparticles which can have diameters between 12-100nm and possess widely different properties.…”

Section: Introductionmentioning

confidence: 99%

Human serum albumin encapsulated gold nanoclusters: effects of cluster synthesis on natural protein characteristics

et al. 2016

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This version is available at https://strathprints.strath.ac.uk/58002/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the Strathprints administrator: strathprints@strath.ac.ukThe Strathprints institutional repository (https://strathprints.strath.ac.uk) is a digital archive of University of Strathclyde research outputs. It has been developed to disseminate open access research outputs, expose data about those outputs, and enable the management and persistent access to Strathclyde's intellectual output. The differences in physiochemical properties between native Human Serum Albumin (HSA) and HSA encapsulated gold nanoclusters (HSA-AuNCs) are characterised. The molecules light absorbance (UV/Vis), electrophoretic mobility, dynamic viscosity, density, hydrodynamic radius (DLS), absorption (QCM) and chemical bonding (XPS) characteristics were studied. The UV/Vis and DLS data shows the formation of large aggregates for HSA-AuNCs between pH 4-6 which is not observed for native HSA. This observation was further supported by QCM measurements showing a large increase in mass adsorbed at pH 6 between HSA and HSA-AuNCs. The DLS data also reveals a hydrodynamic radius of 12nm for HSA-AuNCs, nearly double that of 7nm for native HSA at pH higher than 6, suggesting the formation of compact HSA-AuNCs dimers. The electrophoretic mobility data for both HSA-AuNCs and HSA were converted to zeta potentials. The zeta potential of HSA-AuNCs was seen to be more negative between pH6-12, suggesting that the protein surface is interacting with unreacted gold salt anions. Journal Name ARTICLEMeasurements of density and viscosity were also found to be in agreement with previous data suggesting HSA-AuNCs forms aggregates. XPS data also suggests that not all reactants are used up during the HSA-AuNCs synthesis and positive side chains play a part in the initial synthesis stages. It was concluded that HSA-AuNCs most likely form dimers at natural and high pH.Between pH 4-6 HSA-AuNCs form very large aggregates limiting their use as a fluorescent probe in this pH range. It was also found that the native characteristics of HSA are altered upon HSA-AuNCs synthesis which needs to be taken into consideration when applying HSA-AuNCs as a fluorescent probe in all fluorescent imaging and sensing.

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In vivo self-bio-imaging of tumors through in situ biosynthesized fluorescent gold nanoclusters

Cited by 179 publications

References 46 publications

Facile synthesis of Curcuma longa tuber powder engineered metal nanoparticles for bioimaging applications

Facile synthesis of Curcuma longa tuber powder engineered metal nanoparticles for bioimaging applications

In situSynthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

Human serum albumin encapsulated gold nanoclusters: effects of cluster synthesis on natural protein characteristics

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