In Vivo Silencing of Genes Coding for dTip60 Chromatin Remodeling Complex Subunits Affects Polytene Chromosome Organization and Proper Development in Drosophila melanogaster

Prozzillo, Yuri; Cuticone, Stefano; Ferreri, Diego; Fattorini, Gaia; Messina, Giovanni; Dimitri, Patrizio

doi:10.3390/ijms22094525

Cited by 9 publications

(10 citation statements)

References 44 publications

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“…The unique Drosophila H2A variant, H2Av, combines the features of the H2A.X and H2A.Z eukaryotic variants and has been linked to transcription, DNA repair, and heterochromatin [ 36 ]. H2Av may also have a role in chromosome organization, since the depletion of the machinery responsible for its incorporation results in the alteration of chromosome structure in salivary glands and S2 cells [ 37 , 38 ]. H2Av is broadly distributed in the Drosophila genome and nucleosomes with H2Av are particularly enriched downstream of the transcription start site (TSS) of active genes, which correlates positively with transcription levels [ 25 , 39 ].…”

Section: Chromatin Composition and Structurementioning

confidence: 99%

Chromatin Organization and Function in Drosophila

Llorens-Giralt

Camilleri-Robles

Corominas

et al. 2021

Cells

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Eukaryotic genomes are packaged into high-order chromatin structures organized in discrete territories inside the cell nucleus, which is surrounded by the nuclear envelope acting as a barrier. This chromatin organization is complex and dynamic and, thus, determining the spatial and temporal distribution and folding of chromosomes within the nucleus is critical for understanding the role of chromatin topology in genome function. Primarily focusing on the regulation of gene expression, we review here how the genome of Drosophila melanogaster is organized into the cell nucleus, from small scale histone–DNA interactions to chromosome and lamina interactions in the nuclear space.

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Section: Chromatin Composition and Structurementioning

confidence: 99%

Chromatin Organization and Function in Drosophila

Llorens-Giralt

Camilleri-Robles

Corominas

et al. 2021

Cells

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“…The F1 progeny of these crosses was analysed for chromosome segregation and chromatin integrity. The efficiency of RNAi constructs was already checked by semi-quantitative PCR [4]. Following RNAimediated depletion of the subunits under investigation induced in testes, we found chromatin integrity defects (CID) including condensation and chromosome segregation issues with loss of chromatin fragments (Figure 3 A,B and Table 1).…”

Section: Rnai Depletion Of Dom/tip60 Complex Subunitsmentioning

confidence: 94%

Knockdown of DOM/Tip60 Complex Subunits Impairs Male Meiosis of <em>Drosophila melanogaster</em>

Prozzillo¹,

Fattorini²,

Ferreri³

et al. 2023

Preprint

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ATP-dependent chromatin remodeling complexes are involved in nucleosomes sliding, eviction and/or histone variants incorporation into chromatin to facilitate several cellular and biological processes, including DNA transcription, replication and repair. The DOM/TIP60 chromatin remodeling complex of Drosophila melanogaster contains 18 subunits, including the DOMINO (DOM), an ATPase that catalyzes the ex-change of the canonical H2A with its variant (H2A.V); and TIP60, a lysine-acetyltransferase that acetylates H4, H2A and H2A.V histones. In the last decade, different experimental evidence showed that ATP-dependent chromatin remodeling factors, in addition to their role in chromatin organization, have a functional relevance in cell division. In particular, emerging studies suggested direct roles of ATP-dependent chromatin remodeling complex subunits in controlling mitosis and cytokinesis in both humans and D. melanogaster. However, little is known about their possible involvement during meiosis Meiotic chromosomes non-disjunction led to aneuploid offspring, which are often inviable/poorly viable or sterile due to gene dosage imbalance. Therefore, studying the role of DOM/TIP60 complex in D. melanogaster meiosis can provide new insights on our understanding of the molecular mechanisms underlying cell division control in gametogenesis.

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“…In past studies, we found that the lack of D. melanogaster YETI protein affects chromosome organization and impairs cytokinesis [42][43][44][45][46]. YETI is the Drosophila ortholog of CFDP1 [47][48][49] and belongs to the dTip60 chromatin remodeling complex [50] which includes subunits sharing high sequence and function conservation with those of SRCAP and p400/Tip60 complexes.…”

Section: Subcellular Localization and Rnai-mediated Depletion Of Mrg1...mentioning

confidence: 99%

Evolutionary conserved relocation of chromatin remodeling complexes to the mitotic apparatus

et al. 2022

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Background ATP-dependent chromatin remodeling complexes are multi-protein machines highly conserved across eukaryotic genomes. They control sliding and displacing of the nucleosomes, modulating histone-DNA interactions and making nucleosomal DNA more accessible to specific binding proteins during replication, transcription, and DNA repair, which are processes involved in cell division. The SRCAP and p400/Tip60 chromatin remodeling complexes in humans and the related Drosophila Tip60 complex belong to the evolutionary conserved INO80 family, whose main function is promoting the exchange of canonical histone H2A with the histone variant H2A in different eukaryotic species. Some subunits of these complexes were additionally shown to relocate to the mitotic apparatus and proposed to play direct roles in cell division in human cells. However, whether this phenomenon reflects a more general function of remodeling complex components and its evolutionary conservation remains unexplored. Results We have combined cell biology, reverse genetics, and biochemical approaches to study the subcellular distribution of a number of subunits belonging to the SRCAP and p400/Tip60 complexes and assess their involvement during cell division progression in HeLa cells. Interestingly, beyond their canonical chromatin localization, the subunits under investigation accumulate at different sites of the mitotic apparatus (centrosomes, spindle, and midbody), with their depletion yielding an array of aberrant outcomes of mitosis and cytokinesis, thus causing genomic instability. Importantly, this behavior was conserved by the Drosophila melanogaster orthologs tested, despite the evolutionary divergence between fly and humans has been estimated at approximately 780 million years ago. Conclusions Overall, our results support the existence of evolutionarily conserved diverse roles of chromatin remodeling complexes, whereby subunits of the SRCAP and p400/Tip60 complexes relocate from the interphase chromatin to the mitotic apparatus, playing moonlighting functions required for proper execution of cell division.

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In Vivo Silencing of Genes Coding for dTip60 Chromatin Remodeling Complex Subunits Affects Polytene Chromosome Organization and Proper Development in Drosophila melanogaster

Cited by 9 publications

References 44 publications

Chromatin Organization and Function in Drosophila

Chromatin Organization and Function in Drosophila

Knockdown of DOM/Tip60 Complex Subunits Impairs Male Meiosis of <em>Drosophila melanogaster</em>

Evolutionary conserved relocation of chromatin remodeling complexes to the mitotic apparatus

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