In vivo snapshots of the dopaminergic synapse in small animals

Nikolaus, Susanne; M, Beu; Wirrwar, A.; Antke, Christina; Hw, Müller

doi:10.1038/sj.mp.4001693

Cited by 11 publications

(4 citation statements)

References 16 publications

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“…[ 123 I]iodobenzamide (IBZM) is a SPECT ligand frequently used to study D2R availability in humans. It has also been used in a number of laboratory animals such as rhesus monkeys and rats [5,6]. Although mouse models are important for the study of addiction, studies of the D2R in mice using [ 123 I]IBZM SPECT have not yet been performed.…”

Section: Introductionmentioning

confidence: 99%

SPECT imaging of D2 dopamine receptors and endogenous dopamine release in mice

Jongen

Bruin

Beekman

et al. 2008

Eur J Nucl Med Mol Imaging

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Section: Introductionmentioning

confidence: 99%

SPECT imaging of D2 dopamine receptors and endogenous dopamine release in mice

Jongen

Bruin

Beekman

et al. 2008

Eur J Nucl Med Mol Imaging

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“…In recent investigations, we demonstrated that DAT and D 2 receptor binding and blockade with methylphenidate and haloperidol, respectively, may be quantified in vivo in the rat striatum using a dedicated small-animal SPECT device (17)(18)(19)(20). Additionally, it was shown that increases of synaptic dopamine as induced by methylphenidate may be assessed by quantifying D 2 receptor binding successively under pre-and posttreatment conditions (18).…”

mentioning

confidence: 99%

Pretreatment with Haloperidol Reduces¹²³I-FP-CIT Binding to the Dopamine Transporter in the Rat Striatum: An In Vivo Imaging Study with a Dedicated Small-Animal SPECT Camera

Nikolaus¹,

Antke²,

Kley³

et al. 2009

J Nucl Med

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Synaptic dopamine is mainly regulated by presynaptic dopamine transporter (DAT) activity. We hypothesized that variations in synaptic dopamine are reflected by variations of DAT radioligand binding. The effect of haloperidol, which increases synaptic dopamine concentrations, was therefore assessed in the rat striatum using 123 I-N-v-fluoropropyl-2b-carbomethoxy-3b-(4-iodophenyl)-nortropane ( 123 I-FP-CIT) as a DAT radioligand. Methods: Striatal 123 I-FP-CIT binding was measured in 24 rats under baseline conditions (no pretreatment) and at 1 h after injection of haloperidol or a vehicle (1 mg/kg) using a small-animal SPECT camera. Results: Baseline equilibrium ratios (V 3 $) were 1.32 6 0.24 (mean 6 SD). After the haloperidol injection, V 3 $ decreased to 0.99 6 0.38 (P 2-tailed , 0.0001), corresponding to a mean reduction of DAT binding by 25%. Conclusion: Our results are indicative of competition between the DAT ligand 123 I-FP-CIT and synaptic dopamine elevated by haloperidol, suggesting that the assessment of 123 I-FP-CIT binding may be suitable to study variations in synaptic dopamine in vivo.

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“…In this respect, dedicated PET and SPECT systems that enable imaging of the dopamine system in small laboratory animals are essential assets. Although these systems have shown the feasibility to detect DAT in rats and mice, [10][11][12] these systems lack the spatial and temporal resolution to study fast dynamic aspects of DAT occupancy as a measure of the state of dopaminergic tonus and release parameters in anatomical subdivisions of the forebrain.…”

mentioning

confidence: 99%

Movies of dopamine transporter occupancy with ultra-high resolution focusing pinhole SPECT

et al. 2007

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A pivotal question in neuropharmacology is how the function of neurotransmitter systems relates to psychiatric diseases. In experimental neuropharmacology, we have dreamt about a looking glass that would allow us to see neurotransmitter systems in action, and about animals that would faithfully serve us as models for human psychiatric disease. Analysis of animal models has been limited by the availability of methods to study in vivo neurotransmitter dynamics. Now, a single photon emission computed tomography system called U-SPECT can localize dopamine transporters in sub-compartments of the mouse brain during a range of points in time. Applied to the midbrain dopamine system of different models of disease, this will aid the understanding of dynamic processes of this neurotransmitter that underlie brain functions and human brain pathology.

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In vivo snapshots of the dopaminergic synapse in small animals

Cited by 11 publications

References 16 publications

SPECT imaging of D2 dopamine receptors and endogenous dopamine release in mice

SPECT imaging of D2 dopamine receptors and endogenous dopamine release in mice

Pretreatment with Haloperidol Reduces¹²³I-FP-CIT Binding to the Dopamine Transporter in the Rat Striatum: An In Vivo Imaging Study with a Dedicated Small-Animal SPECT Camera

Movies of dopamine transporter occupancy with ultra-high resolution focusing pinhole SPECT

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In vivo snapshots of the dopaminergic synapse in small animals

Cited by 11 publications

References 16 publications

SPECT imaging of D2 dopamine receptors and endogenous dopamine release in mice

SPECT imaging of D2 dopamine receptors and endogenous dopamine release in mice

Pretreatment with Haloperidol Reduces123I-FP-CIT Binding to the Dopamine Transporter in the Rat Striatum: An In Vivo Imaging Study with a Dedicated Small-Animal SPECT Camera

Movies of dopamine transporter occupancy with ultra-high resolution focusing pinhole SPECT

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About

Pretreatment with Haloperidol Reduces¹²³I-FP-CIT Binding to the Dopamine Transporter in the Rat Striatum: An In Vivo Imaging Study with a Dedicated Small-Animal SPECT Camera