In Vivo Spinal Distribution of Cy5.5 Fluorescent Dye after Injection via the Lateral Ventricle and Cisterna Magna in Rat Model

Lee, Kee-Hang; Nam, Hyunwoo; Won, Jeong-Seob; Hwang, Ji-Yoon; Jang, Hye Won; Lee, Sun-Ho; Joo, Kyeung Min

doi:10.3340/jkns.2017.0252

Cited by 3 publications

(5 citation statements)

References 16 publications

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“…2l, m and Supplementary Table 1 ). Similar to previous studies examining differences in spinal CSF tracer distribution following intraventricular vs. ICM injection 44 , we did not see substantial amounts of tracer in the thoracic or lumbar spine following injection into the cisterna magna.…”

Section: Resultssupporting

confidence: 90%

Gold nanoparticle-enhanced X-ray microtomography of the rodent reveals region-specific cerebrospinal fluid circulation in the brain

et al. 2023

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Cerebrospinal fluid (CSF) is essential for the development and function of the central nervous system (CNS). However, the brain and its interstitium have largely been thought of as a single entity through which CSF circulates, and it is not known whether specific cell populations within the CNS preferentially interact with the CSF. Here, we develop a technique for CSF tracking, gold nanoparticle-enhanced X-ray microtomography, to achieve micrometer-scale resolution visualization of CSF circulation patterns during development. Using this method and subsequent histological analysis in rodents, we identify previously uncharacterized CSF pathways from the subarachnoid space (particularly the basal cisterns) that mediate CSF-parenchymal interactions involving 24 functional-anatomic cell groupings in the brain and spinal cord. CSF distribution to these areas is largely restricted to early development and is altered in posthemorrhagic hydrocephalus. Our study also presents particle size-dependent CSF circulation patterns through the CNS including interaction between neurons and small CSF tracers, but not large CSF tracers. These findings have implications for understanding the biological basis of normal brain development and the pathogenesis of a broad range of disease states, including hydrocephalus.

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Section: Resultssupporting

confidence: 90%

Gold nanoparticle-enhanced X-ray microtomography of the rodent reveals region-specific cerebrospinal fluid circulation in the brain

et al. 2023

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“…Our results are consistent with previous reports. In a previous study, we have injected Cy5.5 fluorescent dye into LV or cisterna magna of rats without SCI and found that LV injection is suitable for the delivery of Cy5.5 fluorescent dye to spinal cords by the CSF circulation [ 11 ]. In this study, we further demonstrate that LV injection is adequate for the distribution of soluble Cy5.5 fluorescent dye and colloidal FMNP-labelled U87MG cells in the entire spinal cord.…”

Section: Discussionmentioning

confidence: 99%

“…Instead, intrathecal injection of stem cells has been considered in clinical trials, expecting stem cells to migrate into disease sites via cerebrospinal fluid (CSF) [ 8 – 10 ]. To simulating clinical situation in animal models, we have injected Cy5.5 fluorescent dye into the lateral ventricle (LV) or cisterna magna (CM) of rat without SCI and compared its in vivo distribution in each region of spinal cord [ 11 ]. LV injection is more suitable than CM injection since it induces widespread distribution of Cy5.5 in spinal cords [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…To simulating clinical situation in animal models, we have injected Cy5.5 fluorescent dye into the lateral ventricle (LV) or cisterna magna (CM) of rat without SCI and compared its in vivo distribution in each region of spinal cord [ 11 ]. LV injection is more suitable than CM injection since it induces widespread distribution of Cy5.5 in spinal cords [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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In vivo distribution of U87MG cells injected into the lateral ventricle of rats with spinal cord injury

et al. 2018

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Stem cells could be the next generation therapeutic option for neurodegenerative diseases including spinal cord injury (SCI). However, several critical factors such as delivery method should be determined before their clinical applications. Previously, we have demonstrated that lateral ventricle (LV) injection as preclinical simulation could be used for intrathecal administration in clinical trials using rodent animal models. In this study, we further analyzed in vivo distribution of cells that were injected into LVs of rats with SCI at thoracic level using in vivo imaging techniques. When 5 × 106 U87MG cells labelled with fluorescent magnetic nanoparticle (FMNP-labelled U87MG) were administrated into LVs at 7 days after SCI, FMNP-labelled U87MG cells were observed in all regions of the spinal cord at 24 hours after the injection. Compared to water-soluble Cy5.5 fluorescent dye or rats without SCI, in vivo distribution pattern of FMNP-labelled U87MG cells was not different, although migration to the spinal cord was significantly reduced in both Cy5.5 fluorescent dye and FMNP-labelled U87MG cells caused by the injury. The presence of FMNP-labelled U87MG cells in the spinal cord was confirmed by quantitative PCR for human specific sequence and immunohistochemistry staining using antibody against human specific antigen. These data indicate that LV injection could recapitulate intrathecal administration of stem cells for SCI patients. Results of this study might be applied further to the planning of optimal preclinical and clinical trials of stem cell therapeutics for SCI.

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“…Özellikle floresan nanopartiküllerin gelecekte daha yaygın olarak in vivo izlemelerde kullanılacakları öngörülmektedir. Lee at al [39] Niad-4 475-480 625-650 Β-amiloid İn vivo çalışmaya uygun Rao et al [15] Bae et al [40] Tokyo green 491 510 Hidrofobik,…”

Section: Floresan ışıMaya Dayalı Görüntüleme Tekniklerinin Avantajlarunclassified

İn vivo floresan görüntüleme ve in vivo uygulamalarda kullanılan florofor bileşikler

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2020

Pamukkale Medical Journal

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Özet Geleneksel olarak kültür ve hücre izlemelerinde kullanılan floresan ışımaya dayalı görüntüleme teknikleri, son on-yıllarda in vivo izlemelerde de kullanılmaya başlanmıştır. Çoğunlukla küçük laboratuvar hayvanlarına uygulanan FLIM, FRET, FRI gibi in vivo görüntüleme düzenekleri hedeflenen bir proteinin veya biyo-molekülün gerçek zaman-mekanda in vivo görüntülerinin alınmasında kullanılırlar. Bu düzenekler genel olarak hayvanın üzerine konacağı sahne, bir floresan uyarıcı ışık kaynağı, gelen ve emisyon ışık filtreleri, kamera aparatı (CCD kamera) ve dataları alıp analiz eden bir yazılım programı elemanlarından oluşmaktadırlar. Floresan işaretleme esasına dayalı çalışan herhangi bir in vivo izleme sisteminin dizaynı iki adımda gerçekleştirilir; öncelikle izleme için hedef seçilen protein veya biyo-molekül uygun bir florofor ile işaretlenir, daha sonra işaretli biyo-molekül canlı bünyede görüntülenir. İn vivo uygulamalarda kullanılan floresan problar; hedef gözetmeyen problar, hedefli aktif problar, hedefli aktive edilebilen problar ve nano-partiküller şeklinde sınıflandırılabilirler. İlave olarak, bir söndürücüye konjuge floresan problar, hedeflerine bağlandıktan sonra quantum verimlerini arttıran florofor problar, organellere spesifik olarak bağlanan floresan problar, iki foton absorbsiyonu esasına dayalı çalışan floresan problar, dönebilen konjuge grup içeren florofor problar ve yakın kızılötesi nanopartiküller diğer önemli floresan prob kategorilerini oluşturmaktadırlar. Genel olarak floresan boyaların hedef/arka-plan ışıma kontrastı, hedefine özgün bağlanma, quantum verimi, absorbsiyon ve emisyon dalga boyları, suda çözünürlük, hücreye nüfuz edebilme ve floresan yaşam-süresi gibi özelliklerinin geliştirilmesi; bu boya maddelerini klinik kullanım açısından elverişli hale getirecektir. Bu derlemede floresan ışımanın mekanizması, floresan ışıma prensibine dayalı olarak çalışan ve önemli görülen in vivo görüntüleme teknikleri ve florofor bileşikler hakkında bilgi verilmesi ve konuyla ilgili önemli çalışmaların gözden geçirilmesi hedeflenmiştir.

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In Vivo Spinal Distribution of Cy5.5 Fluorescent Dye after Injection via the Lateral Ventricle and Cisterna Magna in Rat Model

Cited by 3 publications

References 16 publications

Gold nanoparticle-enhanced X-ray microtomography of the rodent reveals region-specific cerebrospinal fluid circulation in the brain

Gold nanoparticle-enhanced X-ray microtomography of the rodent reveals region-specific cerebrospinal fluid circulation in the brain

In vivo distribution of U87MG cells injected into the lateral ventricle of rats with spinal cord injury

İn vivo floresan görüntüleme ve in vivo uygulamalarda kullanılan florofor bileşikler

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