In Vivo Staining Test with Methylene Blue for Bladder Cancer

Fukui, Iwao; Yokokawa, Masayuki; G, Mitani; Ohwada, Fumio; Wakui, Mamoru; Washizuka, Makoto; Tohma, Tsuguhiro; Igarashi, Kaori; Yamada, Terumasa

doi:10.1016/s0022-5347(17)51088-5

Cited by 50 publications

(21 citation statements)

References 10 publications

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“…This dye has been successfully employed for gross-demarcation of neoplastic tumors in bladder, 21,22 pancreas, 23 and skin. 18,19 MB has also been shown to closely mimic H&E staining pattern of histopathology in vivo 24 and ex vivo.…”

Section: Contrast Agentsmentioning

confidence: 99%

Multimodal optical imaging for detecting breast cancer

et al. 2012

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Abstract. The goal of the study was to evaluate wide-field and high-resolution multimodal optical imaging, including polarization, reflectance, and fluorescence for the intraoperative detection of breast cancer. Lumpectomy specimens were stained with 0.05 mg∕ml aqueous solution of methylene blue (MB) and imaged. Wide-field reflectance images were acquired between 390 and 750 nm. Wide-field fluorescence images were excited at 640 nm and registered between 660 and 750 nm. High resolution confocal reflectance and fluorescence images were excited at 642 nm. Confocal fluorescence images were acquired between 670 nm and 710 nm. After imaging, the specimens were processed for hematoxylin and eosin (H&E) histopathology. Histological slides were compared with wide-field and high-resolution optical images to evaluate correlation of tumor boundaries and cellular morphology, respectively. Fluorescence polarization imaging identified the location, size, and shape of the tumor in all the cases investigated. Averaged fluorescence polarization values of tumor were higher as compared to normal tissue. Statistical analysis confirmed the significance of these differences. Fluorescence confocal imaging enabled cellularlevel resolution. Evaluation and statistical analysis of MB fluorescence polarization values registered from single tumor and normal cells demonstrated higher fluorescence polarization from cancer. Wide-field high-resolution fluorescence and fluorescence polarization imaging shows promise for intraoperative delineation of breast cancers.

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Section: Contrast Agentsmentioning

confidence: 99%

Multimodal optical imaging for detecting breast cancer

et al. 2012

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“…These chromophores are not 100% cancer specific. However, they were reported to be preferentially retained in cancerous tissue [13,14]. TB and MB have been used in clinical practice for staining various carcinomas in vivo [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Multimodal confocal microscopy for diagnosing nonmelanoma skin cancers

2007

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The morphological features and appearance of skin structures in the fluorescence images correlate well with corresponding histology for all investigated tumor-types. Multi-spectral reflectance images provide information on the tissue spectral responses and are complimentary to the fluorescence images. The differences detected by fluorescence polarization in cancerous and normal structures may be used for cancerous tissue discrimination. Our results indicate the feasibility of using multimodal confocal microscopy as real-time tool for detecting skin pathology.

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“…Methylene blue is another recently proposed dye for in vivo staining used in endoscopic examination. Its application has been reported recently in detecting some gastrointestinal abnormalities such as Barrett's esophagus (22)(23)(24) gastric cancer (25) prostate cancers (11,12) and also bladder cancer (26). The exact mechanism for the uptake of methylene blue dye in epithelial cells is still not very clear.…”

Section: Discussionmentioning

confidence: 99%

Methylene Blue as a Diagnostic Aid in the Early Detection of Potentially Malignant and Malignant Lesions of Oral Mucosa

Lejoy¹,

Arpita²,

Krishna³

et al. 2016

Ethiop J Health Sci

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BACKGROUND: In vivo stains are the prompt resources, which have emerged in recent years to aid as clinical diagnostic tools in detecting early potentially malignant and malignant lesions. Toluidine blue, by its property of retaining in the increased DNA and RNA cellular activity areas, aids in delineating the suspicious areas. However, it is hazardous if swallowed, and has been shown to have toxicity to fibroblasts. Methylene blue has a similar chemical structure and exhibits similar physicochemical properties as toluidine blue. It is less toxic to the human body and has recently been proposed for screening some gastrointestinal or prostate tumors. The application of this material in detecting oral lesions has so far not been addressed. The objective of this study was to evaluate the sensitivity and reliability of in vivo staining with methylene blue as a diagnostic adjunct in screening for oral malignant or potentially malignant lesions. METHODS: The present study involved the examination of 75 patients suspected of having oral malignant or potentially malignant lesions by methylene blue staining. The results of methylene blue uptake were compared with a simultaneous biopsy of these lesions. RESULTS: The overall sensitivity was 95% (100% for malignancy and 92% for potentially malignant lesions) and specificity was 70%. The positive predictive value was 91% and negative predictive value of 80% was observed in the study. CONCLUSION: We consider that methylene blue staining is a useful diagnostic adjunct in a large, community-based oral cancer screening program for high-risk individuals.

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In Vivo Staining Test with Methylene Blue for Bladder Cancer

Cited by 50 publications

References 10 publications

Multimodal optical imaging for detecting breast cancer

Multimodal optical imaging for detecting breast cancer

Multimodal confocal microscopy for diagnosing nonmelanoma skin cancers

Methylene Blue as a Diagnostic Aid in the Early Detection of Potentially Malignant and Malignant Lesions of Oral Mucosa

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