In Vivo Stem Cell Imaging

Zhao, Cheng; Tian, Mei; Zhang, Hong

doi:10.2174/1876388x01002010171

Cited by 8 publications

(10 citation statements)

References 74 publications

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“…Therefore, our data about MSC distribution sites and kinetics are consistent with commonly accepted ideas. However, MSC biodistribution studies in vivo are generally limited to 7 to 10 days because the methods of MRI and PET typically used in these studies require labeling of the cells with exogenous contrast agents that are often degraded, diluted, and excreted as cell populations divide [ 23 , 24 ]. In our research, we applied bioluminescence imaging to track MSCs-luc2 cells in living mice and showed survival (and possibly proliferation) of MSCs in the body for at least 7 weeks.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, in vivo optical imaging has been increasingly used to visualize the distribution of transplanted MSCs in the recipient’s body, track their migration to the tumor site, and monitor subsequent proliferation [ 23 ]. Transduction of the stem cells with reporter genes encoding bioluminescent or fluorescent protein provides long-term observation of living cell populations in the animal at the whole-body level [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Influence of mesenchymal stem cells on metastasis development in mice in vivo

et al. 2015

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IntroductionIn recent years, mesenchymal stem cells (MSCs) have been demonstrated to play an important role in carcinogenesis. However, the effect of MSCs on tumor and metastasis development and the mechanisms underlying the interaction of cancer and stem cells are not completely understood. This study investigated the effect of MSCs on breast cancer metastasis formation by using the methods of in vivo fluorescence and luminescence imaging.MethodsMSCs were isolated from bone marrow of normal donors, characterized, and genetically labeled with luciferase (luc2). The effects of MSCs on MDA-MB-231 cancer cell proliferation were evaluated in conditioned medium from MSCs. To generate lung metastases, MDA-MB-231 cells stably expressing red fluorescent protein Turbo FP650 were injected intravenously into nude mice. On day 10 after the cancer cell injection, mice were injected via the tail vein with MSCs-luc2 cells (the MET + MSCs group). Animals that received the injection of MDA-MB-231-Turbo FP650 alone (the MET group) and no injections (the intact control group) served as controls. Fluorescence and bioluminescence imaging was performed for monitoring of the metastasis formation and MSC distribution in the recipient’s body.ResultsWe found that the proliferative activity of the cancer cells in the presence of MSC conditioned medium was lower than that of the cells grown in conventional culture medium. The metastasis formation in the MET + MSCs group was delayed in time as compared with the MET group. Macroscopic and histological examination of isolated lungs 8 weeks after cancer cell injection showed that the total number of metastases in animals of the MET + MSCs group was significantly lower. Using bioluminescence imaging in vivo, we found that MSCs-luc2 cells survived in the host animal for at least 7 weeks and re-migrated to the lung 6 to 7 weeks after injection. Immunohistochemical analysis revealed the presence of MSCs-luc2 in metastases and lung tissue.ConclusionsLong-term in vivo bioluminescence imaging of intravenously injected MSCs-luc2 cells showed distribution of MSCs to the lungs and abdominal organs within the first 2 to 3 weeks and re-migration to the lungs in weeks 6 to 7. It was found that MSCs reduced the proliferative activity of cancer cells in vitro and lung metastasis formation in mice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of mesenchymal stem cells on metastasis development in mice in vivo

et al. 2015

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“…In contrast, fluorescence imaging detects reporter molecule amounts that are in the range of 10 −9 –10 −12 M (Zhao et al, ). Also in favor of bioluminescence is its signal‐to‐noise ratio which is high for luciferase‐based systems, but low for fluorescent proteins (Choy et al, ; Zhao et al, ). Furthermore, as a result of codon optimization, the expression of Luc2, is considerably higher than that of the commonly used wild‐type firefly luciferase.…”

Section: Discussionmentioning

confidence: 99%

Imaging Bioluminescent Exogenous Stem Cells in the Intact Guinea Pig Cochlea

Schomann

Mezzanotte²,

Groot

et al. 2019

The Anatomical Record

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Stem‐cell‐based therapy may be used to replace damaged or lost neurons in the cochlear nerve of patients suffering from severe‐to‐profound sensorineural hearing loss. In order to achieve functional recovery in future clinical trials, knowledge about survival of grafted cells and their differentiation into functional neurons is a prerequisite. This calls for non‐invasive in vivo visualization of cells and long‐term monitoring of their survival and fate after cochlear transplantation. We have investigated if molecular optical imaging enables visualization of exogenous cells in the intact cochlea of guinea pig cadaver heads. Transduced (stem) cells, stably co‐expressing fluorescent (copGFP) and bioluminescent (Luc2) reporter molecules, were injected into the internal auditory meatus or directly into the cochlea through the round window. After injection of the cells into the internal auditory meatus, a bright bioluminescent signal was observed in the cavum conchae of the auricle, indicating that light generated by Luc2 is passing through the tympanic membrane and the external auditory meatus. Similar results were obtained after injection of the cells through the round window membrane, either directly into the scala tympani or in Rosenthal's canal within the modiolus of the basal cochlear turn. Imaging of the auditory bulla demonstrated that the bioluminescent signal passes through the tympanic membrane and crevices in the bony wall of the bulla. After opening the auditory bulla, the bioluminescent signal was emanating from the round window. This is the first study demonstrating that bioluminescence imaging enables visualization of luciferase‐expressing cells injected into the intact guinea pig cochlea. Anat Rec, 303:427–440, 2020. © 2019 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.

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“…Cells are labeled in advance with contrast agents such as gadoliniumdiethylenetriamine penta-acetic acid (Gd-DTPA) for short-term monitoring for upto a week, or superparamagnetic iron oxide (SPIO) for long-term monitoring (reviewed in [102]). Since these contrast agents are exogenous and are often degraded, efforts to develop suitable reporter genes such as the transferrin receptor (TfR) gene that is highly expressed on the target cell membrane have been done for years, however the field is still in its early stage [103,104].…”

Section: Magnetic Resonance Imaging (Mri)mentioning

confidence: 99%