In vivo stem cell tracking with imageable nanoparticles that bind bioorthogonal chemical receptors on the stem cell surface

Lee, Sang‐Min; Yoon, Hwa In; Na, Jin Hee; Jeon, Sangmin; Lim, Seung Woon; Koo, Heebeom; Han, Sang Soo; Kang, Sung Soo; Park, Soon‐Jung; Moon, Sung; Park, Jae Hyung; Cho, Yong Woo; Kim, Byung Soo; Kim, Sang Kyoon; Lee, Taekwan; Kim, Dongkyu; Lee, Seulki; Pomper, Martin G.; Kwon, Ick Chan; Kim, Kwangmeyung

doi:10.1016/j.biomaterials.2017.05.050

Cited by 69 publications

(53 citation statements)

References 67 publications

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“…et al . reported that the metabolic labeling technique can stably label stem cells, but this study only focused on efficient labeling of stem cells 35 .…”

Section: Introductionmentioning

confidence: 99%

Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for Tracking

Han

Shim

Park

et al. 2018

Sci Rep

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Metabolic labeling is one of the most powerful methods to label the live cell for in vitro and in vivo tracking. However, the cellular mechanisms by modified glycosylation due to metabolic agents are not fully understood. Therefore, metabolic labeling has not yet been widely used in EPC tracking and labeling. In this study, cell functional properties such as proliferation, migration and permeability and gene expression patterns of metabolic labeling agent-treated hUCB-EPCs were analyzed to demonstrate cellular effects of metabolic labeling agents. As the results, 10 μM Ac4ManNAz treatment had no effects on cellular function or gene regulations, however, higher concentration of Ac4ManNAz (>20 μM) led to the inhibition of functional properties (proliferation rate, viability and rate of endocytosis) and down-regulation of genes related to cell adhesion, PI3K/AKT, FGF and EGFR signaling pathways. Interestingly, the new blood vessel formation and angiogenic potential of hUCB-EPCs were not affected by Ac4ManNAz concentration. Based on our results, we suggest 10 μM as the optimal concentration of Ac4ManNAz for in vivo hUCB-EPC labeling and tracking. Additionally, we expect that our approach can be used for understanding the efficacy and safety of stem cell-based therapy in vivo.

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“…et al . reported that the metabolic labeling technique can stably label stem cells, but this study only focused on efficient labeling of stem cells 35 .…”

Section: Introductionmentioning

confidence: 99%

Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for Tracking

Han

Shim

Park

et al. 2018

Sci Rep

Self Cite

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“…Again, NIRF‐labeling via DBCO‐Cy5 probe showed notable biocompatibility, in particular over DiD‐based labeling, and allowed efficient in vivo monitoring of intramuscularly administered hASCs for 2 weeks, in particular their co‐localization in ischemic sites. In a follow‐up study, this technology was used to allocate glycol chitosan nanoparticles containing different imaging probes in hASCs . However, rapid uptake of these clickable nanoassemblies was observed within 1 h of incubation.…”

Section: Cell‐rich Assembliesmentioning

confidence: 99%

Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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Bottom‐up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom‐up assembly of unitary living blocks toward the creation of higher order bioarchitectures based on multicellular‐rich structures or multicomponent cell–biomaterial synergies are described. An up‐to‐date critical overview of long‐term existing and rapidly emerging technologies for integrative bottom‐up tissue engineering is provided, including discussion of their practical challenges and required advances. It is envisioned that a combination of cell–biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues for therapies and disease models, as well as tools for fundamental biological studies.

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“…[ 110 ] Bicyclo[6.1.0]nonyne‐modified glycol chitosan nanoparticles (BCN‐CNPs) were also used to deliver imaging agents to human mesenchymal stem cells (hMSCs) in vitro through metabolic engineering of glycans with an azide functionality ( Figure A). [ 111 ] Nanoparticles were internalized by hMSCs and successfully distributed through cell division. In vivo injection of Cy5.5‐, gold‐, and iron‐labeled hMSCs allowed noninvasive cell tracking for up to 15 days through fluorescence imaging and magnetic resonance imaging, demonstrating that Ac 4 ManNAz labeling can be used for prolonged in vivo cell tracking (Figure 5B).…”

Section: Targeting Polymeric Nanoparticles At the Cell Surfacementioning

confidence: 99%

Engineering the Mammalian Cell Surface with Synthetic Polymers: Strategies and Applications

Arno

2020

Macromol. Rapid Commun.

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Manipulating the surface of living cells represents a powerful tool by which to control cell behavior and provides a unique strategy to modulate cellular function and cell–cell interactions. Recent progress in this area has seen the development of robust and elegant approaches to selectively decorate the cell surface, leading to unprecedented advances in cellular manipulation and cell‐based therapies. Despite some impressive in vitro results, several obstacles remain to the broader application of some of these strategies, including their limited translation in vivo. In this review, the leading techniques used to introduce polymers at the plasma membrane of mammalian cells are discussed, focusing on strategies that generate a stable and homogeneous distribution of polymeric chains at the cell surface. Application of these strategies to control cell behavior and deliver cell‐based therapies to targeted tissues are highlighted.

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In vivo stem cell tracking with imageable nanoparticles that bind bioorthogonal chemical receptors on the stem cell surface

Cited by 69 publications

References 67 publications

Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for Tracking

Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for Tracking

Advanced Bottom‐Up Engineering of Living Architectures

Engineering the Mammalian Cell Surface with Synthetic Polymers: Strategies and Applications

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