In vivo studies on the age-dependent decrease of the rates of total and mRNA synthesis in the brain cortex of rats

Semsei, Imre; Szeszák, F; Zs.‐Nagy, Imre

doi:10.1016/0167-4943(82)90004-8

Cited by 41 publications

(8 citation statements)

References 32 publications

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“…It should be noted, in this context, that changes in the chromatin structures are considered to be responsible for the age-associated decrease in transcriptional activity observed in various mammalian tissues including the brain (Medvedev 1984;Richardson et al 1985;Bolla and Denckla 1979;Semsei et al 1982). However, whether there exists a correlation between the age-dependent increase of the N-terminally acetylated and deamidated histone H1°forms and the alterations in gene expression during ageing is unknown.…”

Section: Resultsmentioning

confidence: 90%

Age-dependent deamidation of H1° histones in chromatin of mammalian tissues

Lindner

Sarg

Grunicke

et al. 1999

Journal of Cancer Research and Clinical Oncology

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The composition of the H1(o) histone subfractions was examined in different rat and mouse tissues. Using reverse-phase HPLC and hydrophilic-interaction liquid chromatography we have found that the relative proportions of all four forms of H1(o) differ from tissue to tissue and from species to species. In principle, we observed an age-dependent increase in the amount of both the N-terminally acetylated (H1(o)a Asn-3 and H1(o)a Asp-3) and the deamidated forms of H1(o) (H1(o)a Asp-3 and H1(o)b Asp-3). Compared with the proportion of N-terminally acetylated H1(o) forms in liver, kidney and brain of rats and mice 20 days of age, we found an increase in these H1(o) subfractions of up to 30% in the corresponding organs of 300-day-old animals. The proportion of deamidated H1(o) forms was 1.6- to 4-fold higher in the livers and 8- to 12-fold higher in the brains of 300-day-old mice and rats, respectively, than in 20-day-old animals. The tissue-specific nature of the ratio of H1(o) subfractions suggests that the different forms of histone H1(o) have specific individual functions. The possible biological significance of age-related accumulation of N-terminal acetylated and deamidated histone H1(o) forms is discussed in the light of our results.

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Section: Resultsmentioning

confidence: 90%

Age-dependent deamidation of H1° histones in chromatin of mammalian tissues

Lindner

Sarg

Grunicke

et al. 1999

Journal of Cancer Research and Clinical Oncology

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“…Semsei et a1 (343) Zs.-Nagy and Semsei (443) In vivo -50 Meerson et al (240) Nuclei -37 Liang et al (201) Haji et al (131) Nuclei -25 to -50 In vivo Increase in most tissues Kanungo et al (166) In vivo - 70 Tonna and Singh (396) Fibroblasts - 50 Chen et al (55) Again, one must be concerned with the accuracy in measuring RNA synthesis in the in vivo studies because the specific activities of the precursor pools were not measured. Figure 9.2 shows the effect of age on the synthesis of RNA by cultured fibroblasts isolated from the skin of human subjects over a wide spectrum of ages.…”

Section: Rna Synthesismentioning

confidence: 97%

“…Thus mRNA synthesis appears to decrease with increasing age in rat liver. Semsei et al (343) and Zs.-Nagy and Semsei (443) reported that the in vivo incorporation of radioactive uridine into poly(A)+RNA in brain of rats decreased between 1.5 and 26 months of age. However, the specific activity of the UTP precursor pool was not measured.…”

Section: Rna Synthesismentioning

confidence: 99%

Gene Expression and Protein Degradation

Remmen¹,

Ward²,

Sabia³

et al. 1995

Comprehensive Physiology

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Chromatin proteins and cellular aging The relationship between aging and protein synthesis RNA synthesis Protein synthesis and degradation during aging and senescence Aging and transcription Age-related changes in protein synthesis Enzymes, enzyme alteration, and protein turnover Age-related changes in the structure and function of chromatin Macromolecular methylation during aging The effect of age and nutrition on protein synthesis by cells and tissues from mammals Levels of specific messenger RNA species as a function of age Aging and gene expression Age-related changes of transcription and RNA processing Age-related changes in the synthesis of individual liver-specific proteins Effect of aging on translation and transcription Implication of protein oxidation in protein turnover, aging, and oxygen toxicity Biochemical markers of aging Protein oxidation and aging DNA methylation in aging and cancer RNA and protein metabolism in the aging brain Alterations in gene expression with aging Protein synthesis and the components of protein synthetic machinery during cellular ageing Effect of age on liver protein synthesis and degradation The next frontier: studies of the effects of age and diet on mammalian gene expression Protein synthesis, posttranslational modifications, and aging Gene expression and aging ~ TABLE 9.3. Effect of Age on the mRNA Levels in Liver

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“…The rates of synthesis of total as well as mRNA, measured in vivo by means of radioisotope methods in rats, display an age-dependent decrease in the brain cortex of rats between 1 and 2 years of age (to 40-50% of the young adult value) [96]. In vivo treatments of 60 days with CPH re-increased these rates to 80-90% [97].…”

Section: Evidence For the Oh · Radical-scavenging Properties Of Centrmentioning

confidence: 99%

Aging of Cell Membranes: Facts and Theories

Zs-Nagy

2014

Interdisciplinary Topics in Gerontology and Geriatrics

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This chapter is intended to outline the main results of a research trend realized by the author during the last 45 years, focused on the main role played by the cell membrane in the aging process. It is a very wide field; therefore, the reader cannot expect in this limited space a detailed description, but will be given a wide, interdisciplinary insight into the main facts and theories regarding cellular aging. The central idea described here is the concept called the membrane hypothesis of aging (MHA). The history, the chemical roots, physicochemical facts, biophysical processes, as well as the obligatory biochemical consequences are all touched in by indicating the most important sources of detailed knowledge for those who are more interested in the basic biology of the aging process. This chapter covers also the available anti-aging interventions on the cell membrane by means of the centrophenoxine treatment based on the MHA. It also briefly interprets the possibilities of a just developing anti-aging method by using the recombinant human growth hormone, essential basis of which is the species specificity, and the general presence of receptors of this hormone in the plasma membrane of all types of cells.

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In vivo studies on the age-dependent decrease of the rates of total and mRNA synthesis in the brain cortex of rats

Cited by 41 publications

References 32 publications

Age-dependent deamidation of H1° histones in chromatin of mammalian tissues

Age-dependent deamidation of H1° histones in chromatin of mammalian tissues

Gene Expression and Protein Degradation

Aging of Cell Membranes: Facts and Theories

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