In vivo study of Impact Transplantation Hematopoietic Progenitor Cells on Induced Cutaneous Wound Healing in Rabbits Model

Abstract

The goal of this study was to assess the effects of the Hematopoietic Progenitor Cells (HSCs) on full-thickness cutaneous wounds healing in rabbits. Twenty clinically healthy adult New Zealand White rabbits were used in this study; under aseptic technique and general anesthesia full-thickness excisional cutaneous wound; 20 mm x 20 mm were made on the back. The rabbits were randomly partitioned into two. In first group considered as control group, the wounds were treated with 3 ml of saline solution. In second group (Hematopoietic Progenitor Cells group); wounds were treated with 5x106 hematopoietic progenitor cells. The result of molecular evaluation along the interval of following-up recorded a significant difference of the level Fibroblasts Growth Factor (FGF) and Vascular Endothelial Growth Factor (VEGF) recorded at 7, 14 and 35-day post-surgical operation in hematopoietic progenitor cells group compare than control group. These outcomes were parallel with the finding of the clinical and histopathological assessment showed that hematopoietic progenitor cells group reduces inflammation and promotes epithelialization during 3th weeks with increased vasculature than those in untreated wounds. This study confirms that local implantation of hematopoietic progenitor cells leads to enhance and develop of cutaneous wound healing in rabbit model.

The combined effect of the human umbilical cord blood with chitosan scaffold on the full-thickness wound healing process in rats

“…The current finding agrees with the previous studies, which reported that VEGF and neo-vascularization declined after 21 days beyond injection with BM-MSCs (52,57) and Hematopoietic Progenitor Cells (HSCs) (61). This phenomenon may be related to the decline in blood oxygen supply in the healed skin tissue.…”

Comparación del uso del plasma rico en plaquetas, solo o combinado, sobre el proceso de cicatrización en heridas quirúrgicas

“…Por este método se obtuvieron las heridas con una distancia aproximada de 5 centímetros (cm), diseccionando las 3 capas de la piel (epidermis, dermis e hipodermis). Previo a la realización de las incisiones quirúrgicas en los conejos, ellos fueron tranquilizados con diazepam a dosis de 1 miligramo•kg -1 (mg•kg -1 ), y mezcla de clorhidrato de xilazina a dosis de 5 mg•kg -1 con clorhidrato de ketamina a dosis de 35 mg•kg -1 , administrada por vía intramuscular [29], el mantenimiento se realizó con isoflurano al 3 % con un flujo de oxigeno de 2 litros•minutos -1 (L•min -1 ) a través de mascarilla facial [27].…”

Histopathological study of the effect of using repeated doses of platelets-rich plasma on articular cartilage repair in Rabbits

“…The second spin step was then performed, for the second spin 4000 rpm for 15 minutes should be just effective to help in forming soft pellets at the lower part of the glass tube. The superficial part of the plasma is mainly composing PPP (platelet-poor plasma) which should be removed, and concentrated platelets then homogenized (resuspended) in lower 1/3rd of plasma to create the PRP (17,18).…”