In Vivo Study of the Effects of Propranolol, Timolol, and Minoxidil on Burn Wound Healing in Wistar Rats

Abstract

Propranolol, timolol, and minoxidil have all shown benefits in treatment of burn injury and other skin wounds. The study evaluated their effects on full-thickness thermal skin burns in a Wistar rat model. Performed on 50 female rats; two dorsal skin burns were created on each animal. On the next day, the rats were divided into 5 groups (n=10); each has received a specific treatment daily for 14 days: group I - topical vehicle (control), group II - topical silver sulfadiazine (SSD), group III - oral propranolol (5.5mg) associated with topical vehicle, group IV - topical timolol 1% cream, group V - topical minoxidil 5% cream. Wound contraction rates, malondialdehyde (MDA), glutathione (GSH, GSSG), and catalase activity in skin and/or serum were evaluated, and histopathological analyses were performed. Propranolol did not show advantages in necrosis prevention and wound contraction and healing, and did not reduce oxidative stress. It impaired keratinocyte migration, and promoted ulceration, chronic inflammation, and fibrosis, yet reducing the necrotic zone. Timolol prevented necrosis and promoted contraction and healing, increased antioxidant capacity and promoted keratinocyte migration and neo capillarization in comparison to the other treatments. Minoxidil reduced necrosis and enhanced contraction, resulting in positive outcomes after 1 week of treatment regarding local antioxidant defense, keratinocyte migration, neo capillarization, chronic inflammation, and fibrosis rates. However, after 2 weeks, it resulted in contrasting outcomes. In conclusion, topical timolol promoted wound contraction and healing, reducing local oxidative stress and improving keratinocyte migration, bringing arguments for potential benefits in skin epithelization.

One Molecule, Many Faces: Repositioning Cardiovascular Agents for Advanced Wound Healing

“…In conclusion, intralesional administration of 1% propranolol cream demonstrated potential for promoting re-epithelialization and regulating angiogenesis in diabetic wounds. Freiha et al studied the impact of propranolol, timolol, and minoxidil on full-thickness thermal skin burns in a Wistar rat model [ 62 ]. Fifty rats underwent specific interventions for 14 days: topical vehicle (control), topical silver sulfadiazine, oral propranolol with topical vehicle, topical timolol 1% cream, and topical minoxidil 5% cream.…”