In vivo study of the initial bacterial adhesion on different implant materials

Al‐Ahmad, Ali; Wiedmann-Al-Ahmad, Margit; Fackler, A.; Follo, Marie; Hellwig, Elmar; Bächle, Maria; Hannig, Christian; Han, Jung‐Suk; Wolkewitz, Martin; Kohal, Ralf‐Joachim

doi:10.1016/j.archoralbio.2013.04.011

Cited by 103 publications

(78 citation statements)

References 37 publications

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“…Thus, when the biofilm become mature, the surface roughness seemed to be not the predominate factor that influenced bacteria initial adhesion. This is consistent with previous studies which emphasized that the influence of surface roughness was diminished when the biofilm becomes mature [22,32] on different materials.…”

Section: Discussionsupporting

confidence: 93%

“…Das et al investigated that the maturation adhesion of Streptococcus and Actinomyces strains were in positive relationship with an increase in surface hydrophobicity [33]. The underlying reason may be the fact that increased proteins accumulate on hydrophobic surfaces, which serving as specific binding sites for bacteria, subsequently accelerating and facilitating bacterial adhesion [32]. As observed in our study, the hydrophobicity of zirconia surface was lowest in Group HF and Group GBHF.…”

Section: Discussionsupporting

confidence: 63%

“…Surface texture and topography also play important roles in microbial adhesion [22,32]. Many micrometer-and nanometer-scale topographic patterns with varying shape and size have been shown to inhibit biofilm formation compared to flat surfaces of the same material [29,34].…”

Section: Discussionmentioning

confidence: 99%

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Influence of Grit-Blasting and Hydrofluoric Acid Etching Treatment on Surface Characteristics and Biofilm Formation on Zirconia

et al. 2017

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Abstract:The objective of this study was to investigate the effect of hydrofluoric acid etching treatment on the surface characteristics of zirconia and Streptococcus sanguinis (S. sanguinis) and Porphyromonas gingivalis (P. gingivalis) biofilm formation on zirconia. Zirconia specimens were prepared with different treatments, including being polished with 1000-grit SiC abrasive paper as the control group (Group C), grit-blasted with 110 µm silica-coated alumina particles (Group GB), etched with 40% hydrofluoric acid for 25 min at 100 • C (Group HF), and grit-blasted with 110 µm silica-coated alumina particles and then etched with 40% hydrofluoric acid for 25 min at 100 • C (Group GBHF). The highest surface roughness values and hydrophilicity were shown in Group HF and Group GBHF. Scanning electron microscopy (SEM) showed that hydrofluoric acid can create a crater-like appearance on the zirconia surface. An energy-dispersive X-ray (EDX) analysis demonstrated similar element concentration (wt %) in Group C, Group HF, and Group GBHF, but not for Group GB with higher concentrations of Al and Si element. Colony forming unit (CFU) counts showed that a similar amount of S. sanguinis biofilm and significantly lower P. gingivalis biofilm were formed on zirconia surfaces in Group HF and Group GBHF compared to that in Group C after three days of bacteria culture (p < 0.05). These results indicate that hydrofluoric acid etching on zirconia may not increase S. sanguinis and P. gingivalis mature biofilm formation on zirconia.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 63%

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Influence of Grit-Blasting and Hydrofluoric Acid Etching Treatment on Surface Characteristics and Biofilm Formation on Zirconia

et al. 2017

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“…Moreover, the existing structural differences between Gram-negative and Gram-positive bacteria with regard to the composition of their cell envelope make it necessary to screen both Gram-negative and Gram-positive microorganisms. Nevertheless, in a previous study we found that TB exhibited high bactericidal activity against the initial biofilm formed after 2 h of incubation in the oral cavity (Al-Ahmad et al, 2013a), despite the fact that this initially adherent microbiota consist of both Gram-positive and Gram-negative bacteria (Al-Ahmad et al, 2013b). …”

Section: Discussionmentioning

confidence: 89%

Photoinactivation Using Visible Light Plus Water-Filtered Infrared-A (vis+wIRA) and Chlorine e6 (Ce6) Eradicates Planktonic Periodontal Pathogens and Subgingival Biofilms

et al. 2016

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Alternative treatment methods for pathogens and microbial biofilms are required due to the widespread rise in antibiotic resistance. Antimicrobial photodynamic therapy (aPDT) has recently gained attention as a novel method to eradicate pathogens. The aim of this study was to evaluate the antimicrobial effects of a novel aPDT method using visible light (vis) and water infiltrated infrared A (wIRA) in combination with chlorine e6 (Ce6) against different periodontal pathogens in planktonic form and within in situ subgingival oral biofilms. Eight different periodontal pathogens were exposed to aPDT using vis+wIRA and 100 μg/ml Ce6 in planktonic culture. Additionally, pooled subgingival dental biofilm was also treated by aPDT and the number of viable cells determined as colony forming units (CFU). Live/dead staining was used in combination with confocal laser scanning microscopy to visualize and quantify antimicrobial effects within the biofilm samples. Untreated negative controls as well as 0.2% chlorhexidine-treated positive controls were used. All eight tested periodontal pathogens including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Eikenella corrodens, Actinomyces odontolyticus, Fusobacterium nucleatum, Parvimonas micra, Slackia exigua, and Atopobium rimae and the aPDT-treated subgingival biofilm were eliminated over the ranges of 3.43–8.34 and 3.91–4.28 log10 CFU in the log10 scale, respectively. Thus, aPDT showed bactericidal effects on the representative pathogens as well as on the in situ subgingival biofilm. The live/dead staining also revealed a significant reduction (33.45%) of active cells within the aPDT-treated subgingival biofilm. Taking the favorable tissue healing effects of vis+wIRA into consideration, the significant antimicrobial effects revealed in this study highlight the potential of aPDT using this light source in combination with Ce6 as an adjunctive method to treat periodontitis as well as periimplantitis. The present results encourage also the evaluation of this method for the treatment of caries and apical periodontitis.

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“…The formation of biofilm in oral cavity has four stages: transport of bacteria, initial bacterial adhesion, attachment, and biofilm maturation [12]. Previous studies have evaluated the early adhesion (30 min [15], 1 h [16, 17], 1.5 h [18], 2 h [9], 2.5 h [19], 3 h [20], 4 h [21], and 6 h [22]) on the biomaterial or focused more on the longer exposure (12 h [13], 24 h [9, 20, 23–25], 48 h [17], 2 weeks, and 3 months [26]) in microbiological environment. However, these studies mainly focused on quantification of biofilm accumulation.…”

Section: Introductionmentioning

confidence: 99%

Influence of Surface Properties on Adhesion Forces and Attachment ofStreptococcus mutansto ZirconiaIn Vitro

Wang

Zhou

et al. 2016

BioMed Research International

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Zirconia is becoming a prevalent material in dentistry. However, any foreign bodies inserted may provide new niches for the bacteria in oral cavity. The object of this study was to explore the effect of surface properties including surface roughness and hydrophobicity on the adhesion and biofilm formation of Streptococcus mutans (S. mutans) to zirconia. Atomic force microscopy was employed to determine the zirconia surface morphology and the adhesion forces between the S. mutans and zirconia. The results showed that the surface roughness was nanoscale and significantly different among tested groups (P < 0.05): Coarse (23.94 ± 2.52 nm) > Medium (17.00 ± 3.81 nm) > Fine (11.89 ± 1.68 nm). The contact angles of the Coarse group were the highest, followed by the Medium and the Fine groups. Increasing the surface roughness and hydrophobicity resulted in an increase of adhesion forces and early attachment (2 h and 4 h) of S. mutans on the zirconia but no influence on the further development of biofilm (6 h~24 h). Our findings suggest that the surface roughness in nanoscale and hydrophobicity of zirconia had influence on the S. mutans initial adhesion force and early attachment instead of whole stages of biofilm formation.

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In vivo study of the initial bacterial adhesion on different implant materials

Cited by 103 publications

References 37 publications

Influence of Grit-Blasting and Hydrofluoric Acid Etching Treatment on Surface Characteristics and Biofilm Formation on Zirconia

Influence of Grit-Blasting and Hydrofluoric Acid Etching Treatment on Surface Characteristics and Biofilm Formation on Zirconia

Photoinactivation Using Visible Light Plus Water-Filtered Infrared-A (vis+wIRA) and Chlorine e6 (Ce6) Eradicates Planktonic Periodontal Pathogens and Subgingival Biofilms

Influence of Surface Properties on Adhesion Forces and Attachment ofStreptococcus mutansto ZirconiaIn Vitro

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