In vivo study on the biocompatibility of new resin-based root canal sealers

Kim, Yong-Beom; Baek, Seung‐Ho; Bae, Kwang-Shik

doi:10.5395/jkacd.2002.27.2.122

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…In 0.08 g weight, it had no significant difference with the negative control group regarding cytotoxicity. Kim et al [37] evaluated the biocompatibility of AdSeal compared with AH Plus and AH 26 using the agar diffusion test. Similar to the present findings, they showed that AdSeal was more biocompatible than the other two sealers; however, in contrast to the present results, they reported no cytotoxicity for AdSeal.…”

Section: Discussionmentioning

confidence: 99%

“…Similar to the present findings, they showed that AdSeal was more biocompatible than the other two sealers; however, in contrast to the present results, they reported no cytotoxicity for AdSeal. Some other studies reported higher biocompatibility of AdSeal than AH Plus [37,38]. Although both of these sealers are epoxy resin sealers, higher biocompatibility of AdSeal compared with AH Plus may be due to the presence of calcium phosphate in its composition since calcium phosphate has high biocompatibility and induces bone formation [39].…”

Section: Discussionmentioning

confidence: 99%

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In vitro comparison of the cytotoxicity of different endodontic sealers; AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal

Dastorani¹,

Aliee²,

Halabian³

et al. 2021

Preprint

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Background: This study aimed to assess the cytotoxicity of four commonly used endodontic sealers namely AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal against human gingival fibroblasts (HGFs). Methods: After culturing the HGFs, they were exposed to the respective sealers in set form and in five different weights, after sterilization. The cytotoxicity of the sealers was evaluated after 1, 3 and 7 days using the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed by repeated measures ANOVA. Results: After 24 h, all sealers showed low cytotoxicity. However, all sealers in 250 mg and 500 mg weights showed significantly higher cytotoxicity than the negative control group at 72 h, and 7 days (P<0.05) except for AdSeal in 80 mg weight (P>0.05). AH Plus was significantly more cytotoxic than other sealers at 3 and 7 days (P<0.05) while AdSeal had the closest results to the negative control group, and showed significantly higher biocompatibility than other sealers in 250 mg concentration. Conclusion: AdSeal showed the highest biocompatibility while AH Plus had the highest cytotoxicity among the tested sealers. Thus, its application may delay the healing of periapical lesions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In vitro comparison of the cytotoxicity of different endodontic sealers; AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal

Dastorani¹,

Aliee²,

Halabian³

et al. 2021

Preprint

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“…ADSeal was chosen as a reference group instead of AH Plus (Dentsply Sirona, Konstanz, Germany), the gold standard of epoxy resin sealers, because previous studies showed that this sealer displayed better biocompatibility than AH Plus [ 33 , 34 ]. Although both are epoxy resin sealers, the superior biocompatibility of ADSeal is linked to the presence of calcium phosphate, which induces bone formation [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Sealers on the Cytocompatibility and Osteogenic Potential of Human Periodontal Ligament Stem Cells: An In Vitro Study

Saber

Raafat

Elashiry

et al. 2023

JCM

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Background: There is tendency for unavoidable sealer extrusion in some clinical cases. This might adversely affect host stem cells and affect healing. This study aimed to investigate the effect of different sealers on the cytocompatibility and osteogenic potential of human periodontal ligament stem cells (hPDLSCs). Methods: The cytotoxic effect of the extracted elutes of VDW.1Seal (VDW.1), Endosequence BC Sealer HiFlow (ES), GuttaFlow-2 (GF), and ADSeal (AD-S) on the hPDLSCs was determined using the MTT assay. Cell proliferation and migration were assessed by the scratch wound healing assay. Osteogenic differentiation potential was assessed. Measurement of pH values and calcium ions release was performed. Results: GF had a significantly higher percentage of viable cells. The cell migration assay showed that GF demonstrated the lowest open wound area percentage. GF and AD-S showed the highest calcium nodule deposition. GF demonstrated higher ALP activity than ES. Expression of RUNX2 and OC genes was similar for all sealers, while OPG gene expression was significantly higher for VDW.1 and GF. ES and AD-S displayed the highest pH values on day 1. Calcium ion release of ES and VDW.1 was significantly the highest. Conclusions: GuttaFlow-2 and VDW.1Seal sealers have favorable behavior toward host stem cells.

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