In vivo <sup>13</sup>C NMR studies of glucose catabolism by isolated rumen bacteria

Grivet, Jean‐Philippe; Stevani, J.; Hannequart, Geneviève; Durand, Michelle

doi:10.1051/rnd:19890106

Cited by 8 publications

(6 citation statements)

References 3 publications

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“…For cecectomized birds, the addition of pectin resulted in much greater effects on lactic acid losses than on SCFA losses (Table 3), which confirms that lactic acid production is higher than that of SCFA in the upper tract of chicken (Bensadoun and Ichhponani, 1968). Keeping in mind that the major part of lactic acid is produced before the ceca (Bensadoun and Ichhponani, 1968;Clemens et al, 1975), the observation of much higher lactic acid losses in cecectomized birds than in intact birds (Table 3) suggests the ceca to be a major site for the absorption of lactic acid produced in the upper tract or for its transformation into SCFA (Grivet et al, 1989) before absorption.…”

Section: Resultsmentioning

confidence: 99%

Digestibility of Water-Soluble Pectin and Organic Acid Losses in Intact or Cecectomized Adult Cockerels

Carré

Gomez

1994

Poultry Science

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Two experimental diets consisting of a basal diet either undiluted or diluted with 1% pectin-based gelling agent were given to intact or cecectomized adult cockerels for a balance experiment. The digestibilities of the water-soluble added pectin were 83.2 and 61.4% in intact and cecectomized birds (P < .05), respectively. Lactic acid excretion varied from 407 to 2,692 mg/kg, acetic acid from 790 to 1,214 mg/kg, propionic acid from 108 to 240 mg/kg, and butyric acid from 50 to 90 mg/kg dry matter intake. Organic acid excretions were the lowest for intact birds fed the basal diet and the highest for cecectomized birds fed the pectin diet. Addition of pectin induced an increase (P < .001) in organic acid excretion, especially for lactic acid in cecectomized birds. Organic acid excretion was greater (P < .001) in cecectomized than in intact cockerels. The increase in organic acid excretion induced by pectin addition amounted to 9 and 62% of the digested fraction of pectin in intact and cecectomized cockerels, respectively. The results showed the water-soluble nonstarch polysaccharides to be digested both in ceca and upper tract. It was also shown that the major part of organic acids produced from fermentation are absorbed in the ceca.

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Section: Resultsmentioning

confidence: 99%

Digestibility of Water-Soluble Pectin and Organic Acid Losses in Intact or Cecectomized Adult Cockerels

Carré

Gomez

1994

Poultry Science

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“…[1-'3C]glucose was added to the cell suspensions at a final concentration of 40 mmol I-'. For standardization, late exponential phase cells were washed twice in distilled water and perchloric acid extracts were prepared as described by Grivet et al (1989).…”

Section: Nmr Spectroscopymentioning

confidence: 99%

Glucose metabolism and internal pH of Lactococcus lactis subsp. lactis cells utilizing NMR spectroscopy

Foucaud

Neumann

Hemme

1995

Lett Appl Microbiol

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The metabolism of glucose was studied in Lactococcus lactis subsp. CNRZ 125 by 13C NMR. The initial rate of glucose utilization was higher for exponential phase cells than for stationary phase cells [150 vs 85 nmol g (dry wt)-1 s -1]. 31P NMR was used to determine changes in glycolytic phosphorylated intermediates (fructose-1,6-diphosphate, dihydroxyacetone phosphate and phosphoglycerate). The internal pHs of L. lactis subsp. lactis CNRZ 141 and CNRZ 125 were also measured by 31P NMR as a function of the external pH during growth. When the external pH was 6.8, the internal pHs of strain CNRZ 141 and CNRZ 125 were similar, 7.4. After the external pH had decreased to 5.5, the internal pH of strain CNRZ 141 had declined by 0.6 unit, whereas that of strain CNRZ 125 had decreased by only 0.2 unit of pH.

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“…Here, we report the nutrition-mediated microbial interactions between BL and O157 in a coculture system, taking advantage of a newly developed time-lapse in vivo 2D-NMR profiling method with stable isotope (SI) labeling. The monitoring technique of living cells by NMR is called in vivo NMR which is core technique of our profiling method. − Furthermore, in combination with transcriptomic and proteomic techniques, these methods provide insight to understand the intracellular responses of O157 and BL in the coculture system, as well as their ability to adapt to environmental changes. This two-species system presents a unique coexistence model for examining bacterial crosstalk, and we partially regarded them as a producer and a consumer of nutrients, respectively, especially with regard to serine and aspartate metabolism.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Omics Approach Identifies Nutrition-Mediated Microbial Interactions

et al. 2010

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"Omics" studies reported to date have dealt with either thoroughly characterized single species or poorly explored meta-microbial communities. However, these techniques are capable of producing highly informative data for the analysis of interactions between two organisms. We examined the bacterial interaction between Escherichia coli O157:H7 (O157) and Bifidobacterium longum (BL) as a pathogenic-commensal bacterial model creating a minimum microbial ecosystem in the gut using dynamic omics approaches, consisting of improved time-lapse 2D-nuclear magnetic resonance (NMR) metabolic profiling, transcriptomic, and proteomic analyses. Our study revealed that the minimum ecosystem was established by bacterial adaptation to the changes in the extracellular environment, primarily by O157, but not by BL. Additionally, the relationship between BL and O157 could be partially regarded as that between a producer and a consumer of nutrients, respectively, especially with regard to serine and aspartate metabolism. Taken together, our profiling system can provide a new insight into the primary metabolic dynamics in microbial ecosystems.

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In vivo ¹³C NMR studies of glucose catabolism by isolated rumen bacteria

Abstract: Summary ― Nuclear magnetic resonance (NMR) was used to study the metabolism of the rumen bacterial ecosystem.

Cited by 8 publications

References 3 publications

Digestibility of Water-Soluble Pectin and Organic Acid Losses in Intact or Cecectomized Adult Cockerels

Digestibility of Water-Soluble Pectin and Organic Acid Losses in Intact or Cecectomized Adult Cockerels

Glucose metabolism and internal pH of Lactococcus lactis subsp. lactis cells utilizing NMR spectroscopy

Dynamic Omics Approach Identifies Nutrition-Mediated Microbial Interactions

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In vivo 13C NMR studies of glucose catabolism by isolated rumen bacteria

Abstract: Summary ― Nuclear magnetic resonance (NMR) was used to study the metabolism of the rumen bacterial ecosystem.

Cited by 8 publications

References 3 publications

Digestibility of Water-Soluble Pectin and Organic Acid Losses in Intact or Cecectomized Adult Cockerels

Digestibility of Water-Soluble Pectin and Organic Acid Losses in Intact or Cecectomized Adult Cockerels

Glucose metabolism and internal pH of Lactococcus lactis subsp. lactis cells utilizing NMR spectroscopy

Dynamic Omics Approach Identifies Nutrition-Mediated Microbial Interactions

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In vivo ¹³C NMR studies of glucose catabolism by isolated rumen bacteria