In vivo susceptibility of halophilic and methanogenic organisms to protein synthesis inhibitors

Pecher, T

doi:10.1016/0378-1097(81)90249-4

Cited by 21 publications

(35 citation statements)

References 2 publications

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“…Direct inhibition of the acetone-degrading bacterium by these agents cannot be ruled out at the moment, however, appears to be rather improbable in view of their high specifity for methanogenic bacteria (Gunsalus et al 1978;Sprott et al 1982). Streptomycin, on the other hand, does not inhibit methanogenic bacteria (Pecher and B6ck 1981). An indirect effect via acetate accumulation seems more plausible.…”

Section: Discussionmentioning

confidence: 97%

Methanogenic degradation of acetone by an enrichment culture

1987

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Abstract. An anaerobic enrichment culture degraded 1 mol of acetone to 2 tool of methane and 1 tool of carbon dioxide. Two microorganisms were involved in this process, a filament-forming rod similar to Methanothrix sp. and an unknown rod with round to slightly pointed ends. Both organisms formed aggregates up to 300 gm in diameter. No fluorescing bacteria were observed indicating that hydrogen or formate-utilizing methanogens are not involved in this process. Acetate was utilized in this culture by the Methanothrix sp. Inhibition of methanogenesis by bromoethanesulfonic acid or acetylene decreased the acetone degradation rate drastically and led to the formation of 2 mol acetate per mol of acetone. Streptomycin completely inhibited acetone degradation, and neither acetate nor methane was formed. 1*CO2 was incorporated exclusively into the C-1 atom of acetate indicating that acetone is degraded via carboxylation to an acetoacetate residue. It is concluded that acetone is degraded by a coculture of an eubacterium and an acetateutilizing methanogen and that acetate is the only intermediate transferred between both. The energetical problems of the eubacterium converting acetone to acetate are discussed.

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Section: Discussionmentioning

confidence: 97%

Methanogenic degradation of acetone by an enrichment culture

1987

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“…The antibiotics chosen were those used by both Huser et al (17) and Patel (27). The effect of antibiotics was estimated from methane formation rather than from growth, as described by Pecher and Bock (29), because of the greater sensitivity of methane determinations compared with either turbidity or dry weight measurements. Because methane production was compared with control values, the observed effects were merely related to an inhibition of growth.…”

Section: Figmentioning

confidence: 99%

Description of a New Strain of Methanothrix soehngenii and Rejection of Methanothrix concilii as a Synonym of Methanothrix soehngenii

Touzel¹,

Prensier

Roustan³

et al. 1988

International Journal of Systematic Bacteriology

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A new mesophilic strain of Methanothrix, strain FE, was highly purified from the sludge of an anaerobic digester after enrichment on sodium acetate and is described. Strain FE was compared with other strains of Methanothrix, Methanothrix soehngenii strain OpfikonT (= DSM 2139T) (T = type strain) and Methanothrix concilii strain GP6T (= DSM 3671T). The differences within the strains were mainly related to their requirement for yeast extract. The three strains were found to be similar in their deoxyribonucleic acid guanine-plus-cytosine contents (50.2 to 52.6 mol %) and showed 100 % deoxyribonucleic acid-deoxyribonucleic acid homology. For these reasons we propose to recognize synonymy, reject the name Methanothrix concilii Patel 1985, 35223, and assign this organism to the species Methanothrix soehngenii Huser, Wuhrmann and Zehnder 1983, 33: 439.Methanothrix soehngenii was first isolated by serial dilution from a continuous culture on acetate starting from anaerobically digested sludge in Switzerland (17, 40; B. A.Huser, thesis, Eidgenossischen Teschnischen Hochschule, Zurich, Switzerland, 1981). This bacterium was unique at that time among the methane bacteria because of its ability tlo split acetate into methane and carbon dioxide and its inability to use other methanogenic substrates, especially H,-C02. It was first described as a slow-growing bacterium with doubling times ranging from 5 to 8 days. This bacterium was positioned in the family Methanosarcinaceae by 16s ribosomal ribonucleic acid oligonucleotide sequencing (33). In 1984 (27), Patel described the isolation of Methanothrix concilii, which differed from Methanothrix soehngenii mainly by deoxyribonucleic acid (DNA) base composition and nutritional requirements. In this paper we characterize a new strain of Methanothrix, strain FE, and compare it with the two mesophilic type strains of the genus. MATERIALS AND METHODSSources of strains. Strain FE was initially purified from the sludge of an industrial mesophilic anaerobic contact digester treating wastewaters from the vegetable-canning industry (39). Methanothrix soehngenii strain OpfikonT (= DSM 2139T) (T = type strain) and Methanothrix concilii strain CiP6T (= DSM 3671T) were obtained from the Deutsche Slammlung von Mikroorganismen, Gottingen, West Germany. All of the strains used for immunological studies were also obtained from the Deutsche Sammlung von Mikroorganismen, except for Methanosarcina sp. strains CHTI55, MST-A1, and MC3, which were isolated in our laboratory, and Methanobrevibacter arboriphilicus AZ and Methanosarcina barkeri MST, which were gifts from J. G. Zeikus.Media and culture conditions. Cultivation of methanogens was done on BCYT medium as previously described (37). The medium was buffered with 50 mM HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid) or PIPES [piperazine-l,4-bis(2-ethanesulfonic acid)] unless otherwise indicated. The anaerobic gas was an N,-CO, mixture (85:15). * Corresponding author.The sterile media were inoculated with 2% (vol/vol) portions of previo...

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“…Haloarchaeal strains were selected among all isolates by their susceptibility to anisomycin antibiotic (Santa cruz, USA) (Pecher and Bock, 1981). Antibiotic susceptibility test were studied according to disk diffusion method by the concentration of 30 µg antibiotic per disk.…”

Section: Screening For Haloarchaeamentioning

confidence: 99%

Diversity of hydrolytic enzymes in haloarchaeal strains isolated from salt lake

Makhdoumi

Amoozegar

Khaledi

2011

Int. J. Environ. Sci. Technol.

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ABSTRACT:Production of ten hydrolytic enzymes was qualitatively studied on the haloarchaeal strains isolated from Aran-Bidgol hypersaline lake in the central desert area of Iran. A total of 293 haloarchea strains were selected among 300 extremely halophilic isolated prokaryotes. Accordingly, 142, 141, 128, 64, 38, 16, 7, 3 and 1 archaeal isolates were able to produce DNase, amylase, lipase, inulinase, pullulanase, protease, cellulase, chitinase and xylanase, respectively. None was able to produce pectinase activity. Combined hydrolytic activity was also detected in many strains. A total of 0.3 % of the strains showed 6 hydrolytic activities, 0.3 % of the strains had 5 hydrolytic activities, 5.4 % of the strains presented 4 hydrolytic activities, 25 % of the strains presented 3 hydrolytic activities, 28 % of the strains presented 2 hydrolytic activities and 18 % of the strains presented 1 hydrolytic activity. According to their phenotypic characteristics and comparative partial 16 S rRNA sequence analysis, the halophilic strains were all identified as members of family Halobacteriaceae within 12 different taxa from the following genera: Halorubrum, Haloarcula, Natrinema, Halovivax and Natronomonas. Most enzymes production rate was observed in the genera Halorubrum, Haloarcula and Natrinema whereas; there was not any detectable amount of enzyme production in the genera Halovivax and Natronomonas. The most hydrolytic isolate with 6 combinatorial enzyme production belonged to the genus Natrinema. This investigation showed that the extreme halophilic archaea from Aran-Bidgol lake are a potential source of hydrolytic enzyme under stress conditions and may have possess commercial value.

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In vivo susceptibility of halophilic and methanogenic organisms to protein synthesis inhibitors

Cited by 21 publications

References 2 publications

Methanogenic degradation of acetone by an enrichment culture

Methanogenic degradation of acetone by an enrichment culture

Description of a New Strain of Methanothrix soehngenii and Rejection of Methanothrix concilii as a Synonym of Methanothrix soehngenii

Diversity of hydrolytic enzymes in haloarchaeal strains isolated from salt lake

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