In vivo synthesis of europium selenide nanoparticles and related cytotoxicity evaluation of human cells

Kim, Eun Bee; Seo, Ji Min; Kim, Gi Wook; Lee, Sang Yup; Park, Tae Jung

doi:10.1016/j.enzmictec.2016.08.012

Cited by 34 publications

(18 citation statements)

References 17 publications

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“…Over the past 10 years, many aerobic and anaerobic bacteria have been reported to have the capability of inorganic selenium (SeO 3 2− and/or SeO 4 2− ) reduction with the immediate formation of extra/intracellular SeNPs, e.g., Escherichia coli ATCC 35,218 [53], re-combinant E. coli [54], Ralstoniaeutropha [24,52], Enterobacter cloacae Z0206 [55], Pseudomonas aeruginosa ATCC 27,853 [56], Klebsiella pneumonia [30,31,57,58], Pantoeaagglomerans [14,35], Zooglearamigera [2,25], Rhodopseudomonaspalustris strain N [59], Shewanella sp. HN-41 [60], Azoarcus sp.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of Selenium Nanoparticles (via Bacillus subtilis BSN313), and Their Isolation, Characterization, and Bioactivities

et al. 2021

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Among the trace elements, selenium (Se) has great demand as a health supplement. Compared to its other forms, selenium nanoparticles have minor toxicity, superior reactivity, and excellent bioavailability. The present study was conducted to produce selenium nanoparticles (SeNPs) via a biosynthetic approach using probiotic Bacillus subtilis BSN313 in an economical and easy manner. The BSN313 exhibited a gradual increase in Se reduction and production of SeNPs up to 5–200 µg/mL of its environmental Se. However, the capability was decreased beyond that concentration. The capacity for extracellular SeNP production was evidenced by the emergence of red color, then confirmed by a microscopic approach. Produced SeNPs were purified, freeze-dried, and subsequently characterized systematically using UV–Vis spectroscopy, FTIR, Zetasizer, SEM–EDS, and TEM techniques. SEM–EDS analysis proved the presence of selenium as the foremost constituent of SeNPs. With an average particle size of 530 nm, SeNPs were shown to have a −26.9 (mV) zeta potential and −2.11 µm cm/Vs electrophoretic mobility in water. SeNPs produced during both the 24 and 48 h incubation periods showed good antioxidant activity in terms of DPPH and ABST scavenging action at a concentration of 150 µg/mL with no significant differences (p > 0.05). Moreover, 200 µg/mL of SeNPs showed antibacterial reactivity against Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 9027, and Pseudomonas aeruginosa ATCC 25923. In the future, this work will be helpful to produce biogenic SeNPs using probiotic Bacillus subtilis BSN313 as biofactories, with the potential for safe use in biomedical and nutritional applications.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis of Selenium Nanoparticles (via Bacillus subtilis BSN313), and Their Isolation, Characterization, and Bioactivities

et al. 2021

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“…cysteins, the thiol group of which act as a reducing agent for the formation of NMs (10,16). For these reasons, MT and/or PC have been employed to biosynthesize various metal NMs (13,(18)(19)(20). For example, the biosynthesis of cadmium sulfide NM by recombinant E. coli expressing the Schizosaccharomyces pombe PCS gene and the γ-glutamylcysteine synthetase gene has been reported (13).…”

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confidence: 99%

“…For example, the biosynthesis of cadmium sulfide NM by recombinant E. coli expressing the Schizosaccharomyces pombe PCS gene and the γ-glutamylcysteine synthetase gene has been reported (13). Also, we previously developed a recombinant E. coli strain coexpressing MT and PCS, which allowed biosynthesis of more diverse NMs with higher efficiencies compared with the method employing either MT or PCS alone (SI Appendix, Text S2) (18)(19)(20).…”

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confidence: 99%

Recombinant Escherichia coli as a biofactory for various single- and multi-element nanomaterials

Choi

Park

Lee

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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109

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Nanomaterials (NMs) are mostly synthesized by chemical and physical methods, but biological synthesis is also receiving great attention. However, the mechanisms for biological producibility of NMs, crystalline versus amorphous, are not yet understood. Here we report biosynthesis of 60 different NMs by employing a recombinant strain coexpressing metallothionein, a metal-binding protein, and phytochelatin synthase that synthesizes a metal-binding peptide phytochelatin. Both an in vivo method employing live cells and an in vitro method employing the cell extract are used to synthesize NMs. The periodic table is scanned to select 35 suitable elements, followed by biosynthesis of their NMs. Nine crystalline single-elements of MnO, FeO, CuO, Mo, Ag, In(OH), SnO, Te, and Au are synthesized, while the other 16 elements result in biosynthesis of amorphous NMs or no NM synthesis. Producibility and crystallinity of the NMs are analyzed using a Pourbaix diagram that predicts the stable chemical species of each element for NM biosynthesis by varying reduction potential and pH. Based on the analyses, the initial pH of reactions is changed from 6.5 to 7.5, resulting in biosynthesis of various crystalline NMs of those previously amorphous or not-synthesized ones. This strategy is extended to biosynthesize multi-element NMs including CoFeO, NiFeO, ZnMnO, ZnFeO, AgS, AgTeO, AgWO, HgTeO, PbMoO PbWO, and Pb(VO)OH NMs. The strategy described here allows biosynthesis of NMs with various properties, providing a platform for manufacturing various NMs in an environmentally friendly manner.

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“…In addition, SeNPs have been found to exhibit excellent anticancer and free radical scavenging properties. The biologically synthesized SeNPs have further reduced cytotoxicity and have been tested against various cell lines like Human non-small lung cancer cell line ( Bharathi et al, 2020 ), HeLa (human cervical cancer) and SKOV-3 (human ovarian cancer) cells, ( Kim et al, 2016 ) human keratinocytes ( Matai et al, 2020 ), human breast cancer cells (MCF-7)( Ramamurthy et al, 2013 ). Its cytotoxicity has been found to be less than the most commonly used silver nanoparticle ( Hosnedlova et al, 2018 ; Chudobova et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Biosynthesized selenium nanoparticles: characterization, antimicrobial, and antibiofilm activity against Enterococcus faecalis

Miglani

Tani‐Ishii

2021

PeerJ

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Background Control over microbial growth is a crucial factor in determining the success of endodontic therapy. Enterococcus faecalis is the most resistant biofilm-forming species leading to endodontic failure. Hence, the current researches are directed towards discovering materials with superior disinfection properties and lesser cytotoxicity. This study aimed to synthesize and characterize biogenically produced Selenium Nanoparticles, and to evaluate the antimicrobial and antibiofilm efficacy, against Enterococcus Faecalis, for the following test groups: Group I: Distilled water (control), Group II: SeNPs (1 mg/ml), Group III: Calcium hydroxide (1 mg/ml), Group IV: 2% Chlorhexidine gluconate (CHX), Group V: 5.25% Sodium hypochlorite (NaOCl). Materials and Methods Selenium nanoparticles were derived using fresh guava leaves (Psidium guajava) and were characterized. The antibacterial efficacy against E. faecalis was evaluated by agar well diffusion method. The antibiofilm efficacy of the test groups was observed by viable cell count, antibiofilm assay, and Anthrone and Bradford’s tests. The morphology of the biofilms was analysed using the Scanning Electron Microscope and Fourier Transform Infrared spectroscopy. Results Antibacterial and antibiofilm efficacy of all tested solutions showed superior antibacterial and antibiofilm efficacy when compared to the control group. Overall, SeNPs (Group II) was the most effective against E. faecalis biofilm, followed by NaOCl (Group V), CHX (Group IV), and Ca(OH)2 (Group III). Conclusion Biogenically produced SeNPs emerged as a novel antibacterial and antibiofilm agent against E. faecalis. This nano-formulation demonstrates the potential to be developed as a root canal disinfectant combating bacterial biofilm in endodontics after the results have been clinically extrapolated.

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In vivo synthesis of europium selenide nanoparticles and related cytotoxicity evaluation of human cells

Cited by 34 publications

References 17 publications

Biosynthesis of Selenium Nanoparticles (via Bacillus subtilis BSN313), and Their Isolation, Characterization, and Bioactivities

Biosynthesis of Selenium Nanoparticles (via Bacillus subtilis BSN313), and Their Isolation, Characterization, and Bioactivities

Recombinant Escherichia coli as a biofactory for various single- and multi-element nanomaterials

Biosynthesized selenium nanoparticles: characterization, antimicrobial, and antibiofilm activity against Enterococcus faecalis

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