In vivo targeted gene transfer in skin by the use of laser‐induced stress waves

Ogura, Makoto; Sato, Shunichi; Nakanishi, Kuniaki; Uenoyama, Maki; Kiyozumi, Tetsuro; Saitoh, Daizo; Ikeda, Tomosumi; Ashida, Hiroshi; Obara, Minoru

doi:10.1002/lsm.20024

Cited by 81 publications

(74 citation statements)

References 43 publications

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“…This makes such techniques particularly suitable for the transport of relatively hydrophilic macromolecules, including proteins. Examples of enhanced delivery of macromolecules with these technologies include human growth hormone (radiofrequencies) [89]; gene transfer (lasers) [90]; insulin by pulsed laser [91], sonophoresis [92] or electroporation [93]; vaccines by sonophoresis [94] or electroporation [95]. Disadvantages of cavitational techniques include the possibility of infection due to open pores and pain (particularly with electroporation).…”

Section: Cavitational Technologiesmentioning

confidence: 99%

Delivery of drugs applied topically to the skin

Leite-Silva¹,

Almeida²,

Fradin

et al. 2012

Expert Review of Dermatology

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Section: Cavitational Technologiesmentioning

confidence: 99%

Delivery of drugs applied topically to the skin

Leite-Silva¹,

Almeida²,

Fradin

et al. 2012

Expert Review of Dermatology

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“…14 the gene transfer of a plasmid DNA and the expression level for laser-treated rat skin were two orders higher than the controlled skin; 15 the delivery of gene-coded plasmid into skin grafts was increased and the grafts adhesion was accelerated. 16 Besides, after QS-Nd:YAG laser (532 and 1064 nm) irradiation, both the concentration and penetration depth of methylene blue into biofilms were enhanced; 17 the penetration of 5-fluorouracil into irradiated rabbit ear skin was increased.…”

Section: Introductionmentioning

confidence: 99%

1064 nm-Nd:YAG lasers with different output modes enhancing transdermal delivery: physical and physiological mechanisms

et al. 2013

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Abstract. Lasers have shown great advantages in enhancing transdermal drug delivery. However, the physical or physiological mechanisms are not clear, which limits the application in clinical medicine. Here, 1064 nm-Nd:YAG lasers with long-pulsed (LP, 15 J∕cm 2 ) and Q-switched (QS, 0.5 J∕cm 2 ) output modes inducing short-and long-term effects on the stratum corneum (SC) of skin are investigated. Infrared thermography is applied to monitor the dynamical temperature distribution of the skin surface, while histopathological analysis and two-photon fluorescence microscopy are employed to examine changes in the microstructure of skin and molecular constitution of SC, respectively. Results have shown that the LP laser irradiation increases skin temperature evidently and loosens keratin, making corneocytes fragile or exfoliative, whereas the QS laser irradiation disrupts the keratin or corneocytes completely, perforating some micropores on the SC. It can be concluded that the mechanisms of enhancing transdermal delivery caused by lasers depends on the output modes. The LP laser irradiation produces thermal effects on skin, which loosens the SC, while the QS laser induces mechanical effects on skin, which punches micropores on the SC. Moreover, the laser-induced enhancing effects on transdermal glycerol delivery can last for one week to wait for the recovery of SC. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…Mulholland et al have reported the laser-induced stress wave of a polyimide target, as a direct geometry, interacted with an ArF excimer laser (wavelength 193 nm, full width at half maximum: FWHM 29 ns, energy 350 mJ) [5]. Ogura et al have reported the laser-induced stress wave of a natural rubber target with a Polyethylene terephthalate (PET), as a confined geometry, interacted with a Nd:YAG pulse laser (wavelength 532 nm, FWHM 7.0 ns, laser fluence 1.9 J/cm 2 ) [6]. However, the observed waves in the two geometries have different characteristics in the generation process.…”

Section: Introductionmentioning

confidence: 99%