In vivo toxicity and antimicrobial activity of AuPt bimetallic nanoparticles

Formaggio, Daniela Maria Ducatti; Neto, Xisto Antonio de Oliveira; Rodrigues, Lina Dayse Alcântara; Andrade, Vitor Martins de; Nunes, Bruna Cristina; Lopes-Ferreira, Mônica; Ferreira, Flávia Regina; Wachesk, C. C.; Camargo, Emerson R.; Conceição, Kátia; Tada, Dayane Batista

doi:10.1007/s11051-019-4683-2

Cited by 20 publications

(11 citation statements)

References 48 publications

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“…For example, Piano and coauthors [ 47 , 48 ] showed that the toxicity of 7–20 nm AuNPs to dermal fibroblasts is due to oxidative cell stress. Lastly, some researchers suggested the presence of a synergistic antimicrobial and cytotoxic effect of metal binary composites [ 49 , 50 ]. For example, Li et al [ 49 ] showed that AgNPs were more toxic than AuNPs, whereas the introduction of gold into silver nanoparticles could diminish their environmental impact by reducing the amount of bioavailable Ag.…”

Section: Resultsmentioning

confidence: 99%

“…For example, Li et al [ 49 ] showed that AgNPs were more toxic than AuNPs, whereas the introduction of gold into silver nanoparticles could diminish their environmental impact by reducing the amount of bioavailable Ag. Formaggio and coauthors [ 50 ] examined the toxicity of bimetallic AuPt nanoparticles in vitro and in vivo and evaluated the antimicrobial activity compared to AuNPs and AgNPs. Bimetallic particles showed increased antimicrobial activity compared to AuNPs, but lower activity compared to AgNPs.…”

Section: Resultsmentioning

confidence: 99%

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Noble Metals for Modern Implant Materials: MOCVD of Film Structures and Cytotoxical, Antibacterial, and Histological Studies

et al. 2021

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This work is aimed at developing the modification of the surface of medical implants with film materials based on noble metals in order to improve their biological characteristics. Gas-phase transportation methods were proposed to obtain such materials. To determine the effect of the material of the bottom layer of heterometallic structures, Ir, Pt, and PtIr coatings with a thickness of 1.4–1.5 μm were deposited by metal–organic chemical vapor deposition (MOCVD) on Ti6Al4V alloy discs. Two types of antibacterial components, namely, gold nanoparticles (AuNPs) and discontinuous Ag coatings, were deposited on the surface of these coatings. AuNPs (11–14 nm) were deposited by a pulsed MOCVD method, while Ag films (35–40 nm in thickness) were obtained by physical vapor deposition (PVD). The cytotoxic (24 h and 48 h, toward peripheral blood mononuclear cells (PBMCs)) and antibacterial (24 h) properties of monophase (Ag, Ir, Pt, and PtIr) and heterophase (Ag/Pt, Ag/Ir, Ag/PtIr, Au/Pt, Au/Ir, and Au/PtIr) film materials deposited on Ti-alloy samples were studied in vitro and compared with those of uncoated Ti-alloy samples. Studies of the cytokine production by PBMCs in response to incubation of the samples for 24 and 48 h and histological studies at 1 and 3 months after subcutaneous implantation in rats were also performed. Despite the comparable thickness of the fibrous capsule after 3 months, a faster completion of the active phase of encapsulation was observed for the coated implants compared to the Ti alloy analogs. For the Ag-containing samples, growth inhibition of S. epidermidis, S. aureus, Str. pyogenes, P. aeruginosa, and Ent. faecium was observed.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Noble Metals for Modern Implant Materials: MOCVD of Film Structures and Cytotoxical, Antibacterial, and Histological Studies

et al. 2021

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“…AuNPs were synthesized according to the Turkevich method with adaptations as already reported in our previous work [10]. Firstly, 20 mL of an aqueous solution of HAuCl 4 .3H 2 O at 50 mM was added to 1 L of ultrapure water (type 1) at 95 • C. Then, 10 mL of an aqueous solution of sodium citrate (Na 3 C 6 H 5 O 7 ) at 0.3 M was added.…”

Section: Synthesis Of Aunpsmentioning

confidence: 99%

“…Amongst the metallic nanoparticles, gold nanoparticles (AuNPs) stand out as the most investigated in the last decades. AuNPs have been pointed out as potential agents for imaging, sensoring, photothermia, and antimicrobial applications providing suitable therapeutic efficiency and low toxicity [ 10 , 11 , 12 , 13 ]. The AuNPs have also been reported as useful antitumoral therapeutics, for example, against breast cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Co-Functionalization of Gold Nanoparticles with C7H2 and HuAL1 Peptides: Enhanced Antimicrobial and Antitumoral Activities

et al. 2022

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The functionalization of nanoparticles with therapeutic peptides has been pointed out as a promising strategy to improve the applications of these molecules in the field of health sciences. Peptides are highly bioactive but face several limitations such as low bioavailability due to the difficulty of overcoming the physiological barriers in the body and their degradation by enzymes. In this work, gold nanoparticles (AuNPs) were co-functionalized with two therapeutic peptides simultaneously. The peptides from the complementary determining region of monoclonal antibodies, composed of the amino acid sequences YISCYNGATSYNQKFK (C7H2) and RASQSVSSYLA (HuAL1) were chosen for having exhibited antitumor and antimicrobial activity before. The peptides-conjugated AuNPs were characterized regarding size, morphology, and metal concentration by using TEM, dynamic light scattering, and ICP-OES techniques. Then, peptides-conjugated AuNPs were evaluated regarding the antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. The antitumoral activity was evaluated in vitro by cell viability assays with metastatic melanoma cell line (B16F10-Nex2) and the cytotoxicity was evaluated against human foreskin fibroblast (Hs68) cell line. Finally, in vivo assays were performed by using a syngeneic animal model of metastatic melanoma. Our findings have highlighted the potential application of the dual-peptide AuNPs in order to enhance the antitumor and antimicrobial activity of peptides.

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“…E. coli, for example, is mostly harmless, but under abnormal growth it can cause serious diseases in humans, such as gastroenteritis and infections in the urinary tract, bloodstream and central nervous system. In an attempt to minimize the problem of drug resistance, some alternatives have been considered, such as the development of new antibiotics [22] or the development of alternative therapies such as the therapy based on the use of bioactive peptides [23][24][25]; phytochemicals or nanoparticles [26][27][28]. Antimicrobial photodynamic therapy (aPDT) is another example of alternative therapy that has shown encouraging results in experimental and clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Diamond Nanoparticles-Porphyrin mTHPP Conjugate as Photosensitizing Platform: Cytotoxicity and Antibacterial Activity

Hurtado

Cena

et al. 2021

Nanomaterials

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Conjugation of photosensitizers (PS) with nanoparticles has been largely used as a strategy to stabilize PS in the biological medium resulting in photosensitizing nanoparticles of enhanced photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis (3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond nanoparticles (ND) by covalent bond. Nanoconjugate ND-mTHPP showed suitable stability in aqueous suspension with 58 nm of hydrodynamic diameter and Zeta potential of −23 mV. The antibacterial activity of ND-mTHPP was evaluated against Escherichia coli for different incubation times (0–24 h). The optimal activity was observed after 2 h of incubation and irradiation (660 nm; 51 J/cm2) performed right after the addition of ND-mTHPP (100 μg/mL) to the bacterial suspension. The inhibitory activity was 56% whereas ampicillin at the same conditions provided only 14% of bacterial growth inhibition. SEM images showed agglomerate of ND-mTHPP adsorbed on the bacterial cell wall, suggesting that the antimicrobial activity of ND-mTHPP was afforded by inducing membrane damage. Cytotoxicity against murine embryonic fibroblast cells (MEF) was also evaluated and ND-mTHPP was shown to be noncytotoxic since viability of cells cultured for 24 h in the presence of the nanoconjugate (100 μg/mL) was 78%. Considering the enhanced antibacterial activity and the absence of cytotoxic effect, it is possible to consider the ND-mTHPP nanoconjugate as promising platform for application in antimicrobial photodynamic therapy (aPDT).

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In vivo toxicity and antimicrobial activity of AuPt bimetallic nanoparticles

Cited by 20 publications

References 48 publications

Noble Metals for Modern Implant Materials: MOCVD of Film Structures and Cytotoxical, Antibacterial, and Histological Studies

Noble Metals for Modern Implant Materials: MOCVD of Film Structures and Cytotoxical, Antibacterial, and Histological Studies

Co-Functionalization of Gold Nanoparticles with C7H2 and HuAL1 Peptides: Enhanced Antimicrobial and Antitumoral Activities

Diamond Nanoparticles-Porphyrin mTHPP Conjugate as Photosensitizing Platform: Cytotoxicity and Antibacterial Activity

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