In vivo Toxicological Assessment of the Aqueous Extracts of the Leaves of Carissa edulis (Apocynaceae) in Wistar Rats

Osseni, Razack; Akoha, Stephen; Adjagba, Marius; Azonbakin, Simon; Lagnika, Latifou; Awede, Bonaventure; Bleich, André; Diouf, A.; Darboux, R.; Laleye, Anatóle

doi:10.9734/ejmp/2016/26083

Cited by 13 publications

(8 citation statements)

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“…Our results were similar with those previously published by Adjagba (2015) , Osseni (2016), Adjagba (2017) [26][27][28] who in their work demonstrated that at a dose of 500 mg / kg body weight, the crude aqueous extract of Tridax procumbens, Gmelina arborea and Crateva adansonii significantly reduced MAP in the rat. Considering the model of hypertension used during this study, the mechanism of action of the crude aqueous extract and the active fraction derived from the fresh leaves of Carissa edulis may be a relaxation of the vascular smooth musculature leading to a decrease in peripheral resistances due to the inhibition of the action of L-NAME.…”

Section: Discussionsupporting

confidence: 93%

Effect of fractions from Carissa edulis (Forssk.) Vahl (Apocynaceae) leaves on hypertension induced in Wistars rats

Adjagba¹,

Osseni²,

Adounkpe³

et al. 2022

World J. Adv. Res. Rev.

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High blood pressure is an important health problem because of its prevalence and its several complications. The high cost of its management and the side effects of conventional drugs have led patients to use medicinal plants. Carissa edulis is one of the plants used in the traditional management of hypertension. The aim of this study was to determine the effects of crude extract and fractions of Carissa edulis in L-NAME hypertension rats models. The phytochemical analysis of the extracts was carried out by using the thin layer chromatography method. The pharmacological effect was evaluated in Wistar rats rendered hypertensive by administering the N (ω) -Nitro-L-Arginine-Methyl Ester (L-NAME). The crude extract was administered at 500 mg / kg (b/w) and the fractions at 20 mg / kg (b/w). Blood pressure was measured by a non-invasive method. Four fractions were obtained after fractionation. Many chemical compounds were detected diversely distributed in either in the crude extract or in the fractions. The crude aqueous extract induced a significant decrease in blood pressure from 137, 00 ± 8,01 mmHg to 94,75 ± 2.84 mmHg. In the other hand, only the aqueous fraction exerted the highest effects by reducing the blood pressure from 137,00 ± 8,01 mmHg to 93,5±8,54 mmHg.

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Section: Discussionsupporting

confidence: 93%

Effect of fractions from Carissa edulis (Forssk.) Vahl (Apocynaceae) leaves on hypertension induced in Wistars rats

Adjagba¹,

Osseni²,

Adounkpe³

et al. 2022

World J. Adv. Res. Rev.

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“…Pharmacological screening and toxicological studies assessment are considered as a crucial step before use of drugs. This 90-days subchronic toxicity test is a continuity of the toxicological studies on leaves aqueous extract of the plant (Osseni et al, 2016) since hypertension is a long duration treatment disease. , After 90 days of treatment, no death was recorded in either the control or treated groups.…”

Section: Discussionmentioning

confidence: 99%

Modification of biochemical and haematological parameters during 90-days subchronic toxicity assessment of Carissa edulis in Wistar rats

Osseni¹,

Etienne²,

Adjagba³

et al. 2017

J. Toxicol. Environ. Health Sci.

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A 90-days subchronic toxicity assay was evaluated on the aqueous extract of Carissa edulis leaves. Wistar rats were fed daily with oral doses of 125, 31.25 and 7.87 mg/kg of C. edulis leaves extracts. Toxic effects were assessed using physiological observation, body weight, relative organ weights, feed consumption, biochemical, haematological and histopathological parameters. Many changes of biological values were observed among treated rats versus controls. Although, alterations in biochemical parameters including aspartate amino transferase were observed, in the middle of treatment (day 45), the values were physiologically normalized at the end of the study. Changes were observed also among renal function parameters including increased levels of creatinine, urea and ions disorders. These results were related by the histopathological examinations. The haematological analysis showed an increase in erythrocytes count, haemoglobin, haematocrit (polyerythrocythemia) associated with an increase of MGV level (macrocytosis). These observations suggest that the longterm uses of Carissa edulis could alter some functions of the organism, especially the hepatic, renal and haematopoietic functions. Further specific toxicity studies must be investigated on the plant.

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“… Safe LD 50 - 2154.1 mg/kg. Non-toxic LD 50 > 2000 mg in rats Woode et al (2007) ; Ibrahim et al (2010 , 2015) ; Osseni et al (2016) ; Kaunda and Zhang (2017) Apocynaceae Carissa bispinosa (L.) Desf. ex Brenan Cough Analgesic, antioxidant, antimicrobial, anti-inflammatory, antiviral and diuretic activities.…”

Section: Methodsmentioning

confidence: 99%

Medicinal plants used for the management of respiratory diseases in Zimbabwe: Review and perspectives potential management of COVID-19

Nyagumbo¹,

Pote²,

Shopo³

et al. 2022

Physics and Chemistry of the Earth, Parts A/B/C

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In vivo Toxicological Assessment of the Aqueous Extracts of the Leaves of Carissa edulis (Apocynaceae) in Wistar Rats

Cited by 13 publications

References 0 publications

Effect of fractions from Carissa edulis (Forssk.) Vahl (Apocynaceae) leaves on hypertension induced in Wistars rats

Effect of fractions from Carissa edulis (Forssk.) Vahl (Apocynaceae) leaves on hypertension induced in Wistars rats

Modification of biochemical and haematological parameters during 90-days subchronic toxicity assessment of Carissa edulis in Wistar rats

Medicinal plants used for the management of respiratory diseases in Zimbabwe: Review and perspectives potential management of COVID-19

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