In Vivo Translation of Peptide-Targeted Drug Delivery Systems Discovered by Phage Display

Newman, Maureen R.; Benoit, Danielle S. W.

doi:10.1021/acs.bioconjchem.8b00285

Cited by 28 publications

(23 citation statements)

References 91 publications

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“…This resulted in improvements in drug discovery and vaccine design [ 27 ]. The overview of the phage-display method and its utilization in medicine can be found in recent reviews by Mimmi et al [ 28 ], Sokullu et al [ 29 ], Petrenko [ 30 ], Garg [ 31 ], Sunderland et al [ 32 ], and Newman and Benoit [ 33 ].…”

Section: Bacteriophages In Bio-related Applicationsmentioning

confidence: 99%

Application of Bacteriophages in Nanotechnology

Paczesny

Bielec

2020

Nanomaterials

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Bacteriophages (phages for short) are viruses, which have bacteria as hosts. The single phage body virion, is a colloidal particle, often possessing a dipole moment. As such, phages were used as perfectly monodisperse systems to study various physicochemical phenomena (e.g., transport or sedimentation in complex fluids), or in the material science (e.g., as scaffolds). Nevertheless, phages also execute the life cycle to multiply and produce progeny virions. Upon completion of the life cycle of phages, the host cells are usually destroyed. Natural abilities to bind to and kill bacteria were a starting point for utilizing phages in phage therapies (i.e., medical treatments that use phages to fight bacterial infections) and for bacteria detection. Numerous applications of phages became possible thanks to phage display—a method connecting the phenotype and genotype, which allows for selecting specific peptides or proteins with affinity to a given target. Here, we review the application of bacteriophages in nanoscience, emphasizing bio-related applications, material science, soft matter research, and physical chemistry.

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Section: Bacteriophages In Bio-related Applicationsmentioning

confidence: 99%

Application of Bacteriophages in Nanotechnology

Paczesny

Bielec

2020

Nanomaterials

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“…However, next-generation sequencing (NGS) offers the ability to sequence larger portions of the peptide library (e.g., Illumina sequencing can read over 10 7 sequences) [ 13 ]. Increased sample sizes for sequencing can lead to identification of peptide sequences that may have been under-represented by traditional biopanning and Sanger sequencing, due to poor amplification [ 13 , 14 ]. NGS identifies longer sequences than traditional Sanger sequencing [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…NGS identifies longer sequences than traditional Sanger sequencing [ 15 ]. NGS can also identify peptides based on metrics other than binding affinity, giving researchers more flexibility in utilizing peptides for biomaterial augmentation [ 14 ]. For example, one group used in vivo landscape phage display library in combination with NGS to identify a number of 3-mer consensus sequences or elementary binding units (EBUs) based on peptide avidity, rather than peptide affinity [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…NGS provides more data on the interactions between the peptide library and the desired substrate, making the application of the phage display process more flexible and akin to big data [ 15 ]. With NGS, one could identify peptides that may not necessarily be the highest affinity binders, but may be more infective, better at amplification, or have higher avidity [ 14 , 16 ]. Additionally, NGS can help identify consensus sequences faster than traditional methods and can help identify longer sequences than that generally identified by traditional Sanger sequencing [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Phage Display to Augment Biomaterial Function

Davidson

McGoldrick

Kohn

2020

IJMS

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Biomaterial design relies on controlling interactions between materials and their biological environments to modulate the functions of proteins, cells, and tissues. Phage display is a powerful tool that can be used to discover peptide sequences with high affinity for a desired target. When incorporated into biomaterial design, peptides identified via phage display can functionalize material surfaces to control the interaction between a biomaterial and its local microenvironment. A targeting peptide has high specificity for a given target, allowing for homing a specific protein, cell, tissue, or other material to a biomaterial. A functional peptide has an affinity for a given protein, cell, or tissue, but also modulates its target’s activity upon binding. Biomaterials can be further enhanced using a combination of targeting and/or functional peptides to create dual-functional peptides for bridging two targets or modulating the behavior of a specific protein or cell. This review will examine current and future applications of phage display for the augmentation of biomaterials.

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“…Several technologies have been developed to screen large libraries for protease-substrate profiling, 76 some of which have been successfully applied to prodrug development, such as synthetic combinatorial libraries of fluorogenic substrates, 77,78 positional scanning of synthetic combinatorial libraries, 79,80 substrate phage libraries, 81,82 multiplex substrate profiling by mass spectrometry, 76,[83][84][85] and others. 77,[86][87][88][89][90] Given the enormous substrate diversity of fully randomized peptide libraries, substrate phage display 81 provides an unbiased selection tool to discover cleavable linkers for SACs, which can lead to a manageable set of highly specific substrates for a given protease.…”

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confidence: 99%

Platform to Discover Protease-Activated Antibiotics and Application to Siderophore-Antibiotic Conjugates

Boyce

Dang²,

Ary³

et al. 2020

Preprint

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Here we present a platform for discovery of protease-activated prodrugs and apply it to antibiotics that target Gram-negative bacteria. Because cleavable linkersfor prodrugs had not been developed for bacterial proteases, we used substrate phage to discover substrates for proteases found in the bacterial periplasm. Rather than focusing on a single protease, we used a periplasmic extract to find sequences with the greatest susceptibility to the endogenous mixture of periplasmic proteases. Using a fluorescence assay, candidate sequences were evaluated to identify substrates that release native amine-containing payloads without an attached peptide “scar”. We next designed conjugates consisting of: 1) an N-terminal siderophore to facilitate uptake; 2) a protease-cleavable linker; 3) an amine-containing antibiotic. Using this strategy, we converted daptomycin – which by itself is active only against Gram-positive bacteria – into an antibiotic capable of targeting Gram-negative Acinetobacter species. We similarly demonstrated siderophorefacilitated delivery of oxazolidinone and macrolide antibiotics into a number of Gram-negative species. These results illustrate this platform’s utility for development of protease-activated prodrugs, including Trojan horse antibiotics.

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In Vivo Translation of Peptide-Targeted Drug Delivery Systems Discovered by Phage Display

Cited by 28 publications

References 91 publications

Application of Bacteriophages in Nanotechnology

Application of Bacteriophages in Nanotechnology

Phage Display to Augment Biomaterial Function

Platform to Discover Protease-Activated Antibiotics and Application to Siderophore-Antibiotic Conjugates

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