1990
DOI: 10.1152/ajpgi.1990.259.2.g233
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In vivo transport kinetics and distribution of taurocholate by rat ileum and jejunum

Abstract: The transport kinetics and distribution of taurocholate (TC) from the ileum and jejunum were evaluated in anesthetized Sprague-Dawley rats. Uptake and transport kinetics were determined after simultaneous administration of equimolar solutions of [3H]taurocholic acid ([3H]TC) in the ileum and [24-14C]taurocholic acid ([14C]TC) in the jejunum of anesthetized rats. At TC concentrations between 0.3 and 15 mM, total ileal absorption exceeded jejunal absorption 15- to 25-fold. The apparent Km and Vmax for ileal abso… Show more

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Cited by 17 publications
(17 citation statements)
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“…Since the surface area avail able for absorption and incubation time of TC were lim ited in the present study, it may be speculated that passive transport through the entire small intestine contributes significantly to the enterohepatic circulation of bile salts. This is in keeping with several in vivo experiments [22,26,29], For luminal C-tau concentrations in the range 1-20 mA/, the absorption rate and total absorption of C-tau were approximately 6 to 10-fold higher from the ileum than from the jejunum in the isolated vascularly perfused intestinal segments. This is in keeping with the results obtained by other groups using in vitro and in vivo condi tions [10,20,22,27], In the isolated perfused ileum, the transport process of C-tau was saturable and was strongly inhibited by ouabain.…”
Section: Discussionsupporting
confidence: 90%
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“…Since the surface area avail able for absorption and incubation time of TC were lim ited in the present study, it may be speculated that passive transport through the entire small intestine contributes significantly to the enterohepatic circulation of bile salts. This is in keeping with several in vivo experiments [22,26,29], For luminal C-tau concentrations in the range 1-20 mA/, the absorption rate and total absorption of C-tau were approximately 6 to 10-fold higher from the ileum than from the jejunum in the isolated vascularly perfused intestinal segments. This is in keeping with the results obtained by other groups using in vitro and in vivo condi tions [10,20,22,27], In the isolated perfused ileum, the transport process of C-tau was saturable and was strongly inhibited by ouabain.…”
Section: Discussionsupporting
confidence: 90%
“…The Tmax value recorded in the ex vivo preparation (17.7 nmol-min-1-cm-1) was in the same range as that calculated from in vivo experiments [22,35], These calcu lations provide only an estimate because the Tmax for Ctau transport was not reached when the intraluminal con centration was as high as its critical micellization concen tration. However, using these values and a biliary bile acid secretion rate of 35 pmol-h-1 [26], it can be conclud ed that this low-affinity, high-capacity ileal transport sys tem to absorb bile acids does contribute to conserve the hourly output of bile acids.…”
Section: Discussionsupporting
confidence: 66%
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“…This range of taurocholate concentrations has previously been used successfully to determine kinetics of taurocholate transport in vivo (23).…”
Section: Animalsmentioning
confidence: 99%