In vivo two-photon fluorescent imaging of fluoride with a desilylation-based reactive probe

Abstract

A two-photon excitable molecular probe for fluoride, developed based on a fluoride-specific desilylation reaction, is demonstrated to be useful for fluorescent imaging of fluoride ions in live zebrafish by one-photon as well as two-photon microscopy for the first time.

Selective anion receptor for fluoride detection using ferrocenyl–boronate derivative

“…The detection limit of receptor 1 towards F À was evaluated to be 5.25 mM (99.7 ppb) (Fig. S4), which was well below 4 ppm, the allowed concentration level of F À in drinking water specified by the USEPA [59]. Experiments were explored by increasing the amount of water gradually such as 1%, 2%, 3%, 4%, 5%, 8%, 10%, 15% and 20% aqueous solution on the basis of receptor 1 (1 Â 10 À4 mol L À1 in CH 3 CN) with 10 equivalents' F À ion.…”

An arylboronate-based fluorescent probe for selective and sensitive detection of peroxynitrite and its applications for fluorescence imaging in living cells

“…Thus, it is very promising for arylboronate-based fluorescent probe to realize the fast response toward ONOO  . Based on the consideration, we designed an arylboronate-based fluorescent probe 1, and the design rationale is shown in Scheme 1 and illustrated as follows: (1) an electron-rich diethylamino group was incorporated into the arylboronate reaction group of probe 1 in the hope that such modification could partly decrease the electrophilicity of the boronate group to inhibit the nucleophilic attack of H2O2 to some extent, but has little effect on the strongly nucleophilic ONOO  ; (2) to ensure a big fluorescence off-on response, a covalent-assembly strategy of fluorophore [34][35][36][37] was employed as the fluorescence switching mechanism, by which the highly fluorescent iminocourmarin 5 was expected to be produced via the ONOO  -triggered oxidative hydrolysis followed by intramolecular cyclization.…”

A ratiometric two-photon fluorescent probe for fluoride ion imaging in living cells and zebrafish

“…3 Unfortunately, the in vivo imaging of fluoride ions in biological samples is still a crucial challenge. [16][17][18][19][20][21][22][23][24][25][26][27][28] Among these mechanisms, the Si-O bond cleavage based mechanism is favorable for ratiometric sensing performances. 4 Recently, various fluorescent probes have been reported for fluoride ions based on the sensing mechanism of Lewis acid-base coordination 5-9 , anion-π interaction 10-15 and Si-O bond cleavage.…”