In vivo versus in vitro produced bovine ova: similarities and differences relevant for practical application

Holm, Preben Bach; Callesen, Henrik

doi:10.1051/rnd:19980601

Cited by 73 publications

(31 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The identification of novel genes involved in blastocyst formation and the analysis of their expression patterns may help us to understand the mechanisms that control blastocyst formation and may help us to understand why certain embryos do not make it through this stage. As in vitro culture conditions do not fully mimic the in vivo situation and as it has been shown that morphological and physiological differences [5-7] as well as differences in gene expression [8-11] exist between in vivo and in vitro cultured embryos, the RNA expression levels of in vitro produced embryos should be compared with those of in vivo "golden standard" embryos. Changes in transcript abundance between in vitro and in vivo embryos may help to assess the normality of in vitro produced embryos and may help to optimise the in vitro culture conditions.…”

Section: Introductionmentioning

confidence: 99%

Identification and expression analysis of genes associated with bovine blastocyst formation

et al. 2007

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Identification and expression analysis of genes associated with bovine blastocyst formation

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Despite recent improvements in bovine in vitro production (IVP) systems, IVP embryos display marked differences compared with their in vivo counterparts (for reviews, see [6][7][8]. The in vitro culture system has profound effects on mRNA expression patterns of developmentally important genes [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Production and Nuclear Transfer Affect Dosage Compensation of the X-Linked Gene Transcripts G6PD, PGK, and Xist in Preimplantation Bovine Embryos1

Wrenzycki

Lucas‐Hahn

Herrmann

et al. 2002

Biology of Reproduction

174

104

View full text Add to dashboard Cite

Equal expression of X-linked genes such as G6PD and PGK in females and males and the initiation of X-chromosome inactivation are critically dependent on the expression of the X-inactive specific transcript (Xist). The objective of the present study was to determine the effects of in vitro production (IVP) and nuclear transfer (NT) on the relative abundance (RA) of the X-linked transcripts G6PD, PGK, and Xist in preimplantation bovine embryos. In experiment 1, sex-determined IVP or in vivo-produced embryos were analyzed for mRNA expression of the 3 genes. The sex ratio was 36% vs. 64% in IVP blastocysts and thus deviated significantly from the expected ratio of 50% in the vivo control group. The RA of G6PD transcripts was significantly higher in female IVP embryos than in male embryos. In contrast, no significant differences were seen between in vivo-derived female embryos and their male counterparts. At the morula stage, female IVP embryos transcribed significantly more PGK mRNA than did male embryos. However, blastocysts did not exhibit significant differences in PGK transcripts. No differences were observed for in vivo-derived embryos with regard to the RA of PGK transcripts. The RA of Xist mRNA was significantly higher in all female embryos than in their male counterparts. In experiment 2, IVP, in vivo-developed, NT-derived, and parthenogenetic embryos carrying two X chromosomes of either maternal and paternal origin or of maternal origin only (parthenogenotes) were analyzed for the RA of the 3 genes. In NT-derived morulae, the RA of G6PD transcripts was significantly increased compared with their IVP and in vivo-generated counterparts. G6PD transcript levels were significantly increased in IVP blastocysts compared with in vivo-generated and parthenogenetic embryos. At the morula stage, PGK transcripts were similar in all groups, but the RA of PGK transcripts was significantly higher in IVP blastocysts than in their in vivo-generated, parthenogenetic, and NT-derived counterparts. The RA of Xist was significantly elevated in NT-derived morulae compared with IVP, in vivo-generated, and parthenogenetic embryos. NT-derived blastocysts showed an increased Xist expression compared with that of IVP, in vivo-generated, and parthenogenetic embryos. Results of the present study show for the first time that differences in X-chromosome-linked gene transcript levels are related to a perturbed dosage compensation in female and male IVP and female NT-derived embryos. This finding warrants further studies to improve IVP systems and NT protocols to ensure the production of embryos with normal gene expression patterns.

show abstract

“…Although significant progress has been made in assisted reproductive technology, during the last two decades multiple ovulations by gonadotrophin treatments still represents the greater source of embryos for all technologies connected to embryo transfer programs in domestic species [15].…”

Section: Introductionmentioning

confidence: 99%

Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment

et al. 2001

View full text Add to dashboard Cite

-In the non breeding period, the effect of two superovulatory treatments (eCG/FSH in single dose or FSH alone in four decreasing doses) on the production of embryo quality following in vitro viability after vitrification procedures was investigated using forty-four adult Sarda breed ewes. In sheep treated with eCG/FSH, the mean number of corpora lutea was significantly (P < 0.05) higher (11.8 ± 4.0 vs. 8.05 ± 3.8), although the recovery rate was significantly (P < 0.01) lower (74.6 vs. 59.9) than with FSH alone. After vitrification (ethylene glycol and glycerol) was repeated three times, the rates of re-expansion at first and second warming were significantly (P < 0.01) higher in embryos derived from FSH alone than in those with both gonadotrophins (94.9 and 41.9 vs. 72.8 and 18.6) and after the last vitrification the hatched blastocyst rates were 22.5 and 7.6. After differential stain, blastocysts derived from FSH alone showed a mean number of cells significantly higher than blastocysts from eCG/FSH (184.2 vs. 157.7). It was concluded that superovulatory treatment with eCG/FSH may increase the ovarian responses compared with FSH alone, but these embryos showed a reduction in viability rates after repeated vitrification. superovulation / embryo quality / ovine / vitrification / viabilityRésumé -Les embryons ovins obtenus après un traitement de superovulation associant FSH et eCG ont une viabilité in vitro plus faible après vitrification que les embryons obtenus avec un traitement séquentiel de FSH. Pendant la saison non reproductive, on a évalué, chez 44 brebis adultes de race Sarde, l'effet de deux traitements de superovulation (le premier avec les eCG/FSH en une seule administration, le deuxième avec la FSH administrée en quatre doses décroissantes) sur la qualité des embryons produits et sur la viabilité in vitro après vitrification. Dans le groupe traité avec les eCG/FSH, le nombre moyen d'ovulations était plus élevé (11,8 ± 4,0 vs. 8,05 ± 3,8, P < 0,05) tandis que le taux de récupération était plus faible (74,6 vs. 59,9, P < 0,01) par rapport au groupe traité avec la FSH seule. Les embryons ont suivi 3 vitrifications successives (éthylène glycol et glycérol). Reprod. Nutr. Dev. 41 (2001) [239][240][241][242][243][244][245][246] 239

show abstract

In vivo versus in vitro produced bovine ova: similarities and differences relevant for practical application

Abstract: -This present review describes some differences and similarities between bovine embryos produced in vivo and in vitro.

Cited by 73 publications

References 47 publications

Identification and expression analysis of genes associated with bovine blastocyst formation

Identification and expression analysis of genes associated with bovine blastocyst formation

In Vitro Production and Nuclear Transfer Affect Dosage Compensation of the X-Linked Gene Transcripts G6PD, PGK, and Xist in Preimplantation Bovine Embryos1

Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment

Contact Info

Product

Resources

About