In vivo viability and functional integrity of filtered platelets

Abstract

The in vivo viability and functional integrity of filtered platelets were compared with those of nonfiltered platelets in a controlled study. On two occasions, after template bleeding time, 14 healthy volunteers underwent plateletpheresis and received 600 mg of aspirin. Autologous 111In-labeled platelets were transfused without further manipulation (control) on one occasion and after filtration on a second occasion. The filter was primed and flushed with a buffered 12.6-percent solution of ACD-A in 0.9-percent normal saline (pH 6.5). After transfusion, the bleeding time was measured at 1, 4, and 24 hours and platelet survival at 10 minutes; 1, 4, and 24 hours; and daily for 6 days. The decrease in the bleeding time was not significantly different from that after transfusion of nonfiltered platelets (p greater than 0.2). Filtering of platelets did not affect 1-hour in vivo recovery (filtered, 69.5%; nonfiltered, 66%: p = 0.56) or the platelet survival (filtered platelet t1/2 = 83.0 hours, nonfiltered platelet t1/2 = 82.9 hours: p = 0.96). It can be concluded that filtration does not adversely affect in vivo recovery, survival, or functional integrity of platelets.

Circulation and hemostatic function of autologous fresh, liquid‐preserved, and cryopreserved baboon platelets transfused to correct an aspirin‐induced thrombocytopathy

“…Some investigators have questioned the rationale of comparing the ability of platelets to correct an aspirin‐induced thrombocytopathy in normal volunteers with platelet function in the treatment of thrombocytopenic patients 31,32 . Results from our previous studies in human volunteers 8‐10 and from this study in the baboon are similar to those reported by Khuri et al 7 when cardiopulmonary bypass patients received transfusion of allogeneic liquid‐preserved platelets stored for a mean of 3.4 days and allogeneic previously frozen washed platelets.…”

Nonsurgical bleeding diathesis in anemic thrombocytopenic patients: role of temperature, red blood cells, platelets, and plasma‐clotting proteins

“…Contrary to that assumption, our studies of autologous fresh, liquid‐preserved, or cryopreserved PLTs in aspirin‐treated baboons showed no correlation between the survival of the preserved non–aspirin‐treated PLTs and their function to reduce the prolonged BT and increase the thromboxane level in the shed blood 167 . Pineda and associates 200 and Brecher and associates 201 have compared the survival and function of various PLT products in human aspirin‐treated volunteers. They studied autologous non–aspirin‐treated washed and nonwashed PLTs and filtered PLTs and nonfiltered PLTs.…”

“…Findings from a prospective randomized study in CPB surgery patients comparing the survival and function of washed previously frozen PLTs to values for liquid‐preserved PLTs indicated that results were similar to those observed when washed previously frozen PLTs and liquid‐preserved PLTs were transfused to aspirin‐treated human volunteers and baboons 162‐168,184 . We suggest that this lends credence to studies comparing the survival and function of autologous preserved PLTs in aspirin‐treated human volunteers 162‐166,200,201 …”

“…The survival and function of preserved PLTs are necessary to restore the hemostatic defect in patients with reduced PLT counts and/or with PLT dysfunction. The survival and function of autologous preserved PLTs can be assessed in aspirin‐treated normal volunteers 162‐166,200,201 . The in vivo interaction of RBCs and PLTs provides hemostasis by the PLTs reducing blood flow by producing thromboxane and the RBCs scavenging the endothelial cell nitric oxide responsible for vasodilation of blood vessels and the inhibition of PLT function 135 .…”

Platelet radiolabeling procedure

“…In our studies of autologous fresh, liquid‐preserved, or cryopreserved PLTs in aspirin‐treated baboons, we observed no correlation between the survival of the preserved non–aspirin‐treated PLTs and their function to reduce the prolonged bleeding time and increase the thromboxane level in the shed blood 6 . Pineda and associates 8 and Brecher and associates 9 have compared the survival and function of autologous non–aspirin‐treated washed and nonwashed PLTs and of filtered PLTs and nonfiltered PLTs in aspirin‐treated human volunteers. Investigators have questioned the relevance of the studies of the survival and function of non–aspirin‐treated preserved PLTs in aspirin‐treated human volunteers and aspirin‐treated baboons to the survival and function of preserved PLTs to treat anemic and thrombocytopenic patients 7 .…”