In Vivo Visualization of Endolymphatic Hydrops in Guinea Pigs: Magnetic Resonance Imaging Evaluation at 4.7 Tesla

Zou, Jing; Pyykkö, Ilmari; Bretlau, P; Klason, Tomas; Bjelke, Börje

doi:10.1177/000348940311201212

Cited by 58 publications

(49 citation statements)

References 10 publications

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“…There is potential to use this method to visualize endolymphatic hydrops and the permeability change or rupture of Reissner’s membrane, as has been done in guinea pigs [14]. This can be improved with a better coil structure.…”

Section: Discussionmentioning

confidence: 99%

“…The uptake of Gd in the cochlear chambers was monitored in 10-min intervals over a period of 40–90 min following administration. All the parameters were the same as previously reported [14]. The field of view was 3 cm, slice thickness 0.5 mm, centre-centre separation 0.65 mm, acquisition matrix 256 × 256 and reconstruction matrix 512 × 512, recovery time 500 ms and repetition time 693.1 ms. RARE factor was 32, time between refocusing pulses 6.1 ms and phase encoding gradient increment such as to yield an effective echo time of 31.5 ms.…”

Section: Methodsmentioning

confidence: 99%

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Communication between the Perilymphatic Scalae and Spiral Ligament Visualized by in vivo MRI

Pyykkö²,

et al. 2005

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We evaluated the transport of Gadolinium-diethylenetriaminepentaacetate-bismethylamide (Gd-DTPA-BMA) through the round window (RW) membrane into the perilymphatic space with 4.7-T MRI in an animal study and 1.5-T MRI in humans. After administration of Gd-DTPA-BMA onto the intact RW membrane of guinea pig, Gd-DTPA-BMA uptake was observed in the basal turn and part of the second turn within 40 min. The scala tympani, scala vestibuli, the fibrous part of the spiral ligament and semicircular canal all showed uptake of Gd-DTPA-BMA. All turns of the cochlea were filled with Gd within 10 min in the perforated RW membrane administration group and within 30 min in the intravenous administration group. In patients who accepted middle ear injection of Gd-DTPA-BMA, uptake was observed within 2 h in the basal turn and semicircular canal. After 12 h the apex did still not show any uptake. Gd-DTPA-BMA is transported from the RW to the semicircular canal, the scala tympani and scala vestibuli without passing the helicotrema.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Communication between the Perilymphatic Scalae and Spiral Ligament Visualized by in vivo MRI

Pyykkö²,

et al. 2005

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“…The advantage of a sequence with strong T1-weighting, such as FLASH used in this work and FLAIR (1,2), is that it can be used to study gadolinium concentrations that change between cochlear chambers at different times between injection and imaging (5); the disadvantage is that gadolinium injection is very invasive (7). CISS can obtain high SNR images without gadolinium injection, but suffers from banding artifacts (7) that complicate image interpretation, so is used primarily for obtaining anatomical reference images (1,2).…”

Section: Discussionmentioning

confidence: 99%

An improved RF and gradient coil system for high resolution in vivo guinea pig cochlea imaging on a 3T clinical magnet

Kaggie

Goodrich

Kim

et al. 2014

Concepts Magn Reson

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Purpose: The purpose of this work was to develop and evaluate a system consisting of an insertable gradient, RF coils, and animal positioning hardware to enable imaging of the internal structure of the cochlea and other inner ear structures of a guinea pig model at 3T.Methods: Transmit and receive RF coils, and an animal positioning system, were developed and integrated with an insertable gradient and animal monitoring system for improved guinea pig cochlea imaging when compared to a standard clinical 3T MRI system. A resolution phantom with 120 lm line separations was used to assess the contrast improvements with the insertable gradient. A homogeneous phantom was used to assess the SNR and homogeneity of the RF coils. FLASH imaging was used to observe the temporal passage of gadolinium contrast through the guinea pig cochlea. Results:The insertable gradient enabled increased resolution imaging with nearly twice the contrast-to-noise ratio. The small animal array had better SNR for imaging the guinea pig cochlea (about 14 mm deep) than the commercial Siemens wrist coil (2.23) and the small birdcage coil (1.63). The positioning device kept the animal secure and stationary. Injected gadolinium contrast allowed visualization of the internal cochlear structures with FLASH image acquisition, which achieved 100 lm isotropic resolution images in 33 min. Conclusion:The specialized RF coils and animal holding equipment designed to operate within the composite gradient enabled higher resolution images than could be obtained from the available RF coils operating in the conventional gradients on the clinical MRI system.

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“…Gadolinium-enhanced inner ear MRI became a valuable diagnostic tool in inner ear diseases after two milestone studies showed that the cochlear scalae were distinguishable, and that the experimental endolymphatichy drops was visible in vivo in 4.7 T MRI after intravenous injection of Gadolinium Diethylenetriaminepentaacetic Acid Bismethylamide (Gd-DTPA-BMA) in guinea pigs [1][2][3]. In the studies, high-dose Gadolinium chelate (GdC) was applied intravenously to induce enough distribution in the inner ear because GdC has poor transport efficacy across the blood-perilymph barrier [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Targeted Delivery of Contrast Agents to the Tympanic Medial Wall at Minimum Amount and the Efficient Uptake in the Inner Ear through Oval and Round Windows

Zou¹

2015

Austin J Radiol

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Inner ear MRI after intratympanic administration of Gadolinium chelate (GdC) has become a valuable method in detecting endolymphatichy drops attributing to the efficient uploading in the inner ear while avoiding high dose intravenous application that can cause renal injury. In addition to the well-accepted round window pathway, the oval window was discovered as a dominant approach for the agents to move from the middle ear into the inner ear. In order to fine-tune this method, a targeted delivery of GdC to the medial wall of the middle ear cavity was developed in a minimally invasive way. The targeted tympanic medial wall administration was expended in the topical tympanic application of super paramagnetic iron oxide nanoparticles and manganese as contrast agents in the inner ear MRI. This review provides an overview of the developments in the field.

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In Vivo Visualization of Endolymphatic Hydrops in Guinea Pigs: Magnetic Resonance Imaging Evaluation at 4.7 Tesla

Cited by 58 publications

References 10 publications

Communication between the Perilymphatic Scalae and Spiral Ligament Visualized by in vivo MRI

Communication between the Perilymphatic Scalae and Spiral Ligament Visualized by in vivo MRI

An improved RF and gradient coil system for high resolution in vivo guinea pig cochlea imaging on a 3T clinical magnet

Targeted Delivery of Contrast Agents to the Tympanic Medial Wall at Minimum Amount and the Efficient Uptake in the Inner Ear through Oval and Round Windows

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