In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives

Noguchi, Asako; Ikegaya, Yuji; Matsumoto, Nobuyoshi

doi:10.3390/s21041448

Cited by 28 publications

(12 citation statements)

References 225 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, patch-clamp electrophysiology provides information about single-cell neuronal activity by studying real-time current data generated by ion channels [ 81 ]. This technique allows researchers to assess neuronal activity at the microscopic level.…”

Section: Biomarkers Of Epileptogenesismentioning

confidence: 99%

The Neurovascular Unit Dysfunction in the Molecular Mechanisms of Epileptogenesis and Targeted Therapy

Liu,

Zhang,

Zhao

et al. 2024

Neurosci. Bull.

View full text Add to dashboard Cite

Epilepsy is a multifaceted neurological syndrome characterized by recurrent, spontaneous, and synchronous seizures. The pathogenesis of epilepsy, known as epileptogenesis, involves intricate changes in neurons, neuroglia, and endothelium, leading to structural and functional disorders within neurovascular units and culminating in the development of spontaneous epilepsy. Although current research on epilepsy treatments primarily centers around anti-seizure drugs, it is imperative to seek effective interventions capable of disrupting epileptogenesis. To this end, a comprehensive exploration of the changes and the molecular mechanisms underlying epileptogenesis holds the promise of identifying vital biomarkers for accurate diagnosis and potential therapeutic targets. Emphasizing early diagnosis and timely intervention is paramount, as it stands to significantly improve patient prognosis and alleviate the socioeconomic burden. In this review, we highlight the changes and molecular mechanisms of the neurovascular unit in epileptogenesis and provide a theoretical basis for identifying biomarkers and drug targets.

show abstract

Section: Biomarkers Of Epileptogenesismentioning

confidence: 99%

The Neurovascular Unit Dysfunction in the Molecular Mechanisms of Epileptogenesis and Targeted Therapy

Liu,

Zhang,

Zhao

et al. 2024

Neurosci. Bull.

View full text Add to dashboard Cite

show abstract

“…Cellular electric neural activity can be accurately measured using traditional electrode measurements, such as the patch clamp method. This method is highly accurate, but is generally limited to single-cell recordings and invasive to cells [ 144 , 145 , 146 ]. Calcium has also been widely used to reflect neuronal electric activities.…”

Section: Genetically Encoded Tools That Can Be Used For Studying Deve...mentioning

confidence: 99%

Bioelectricity in Developmental Patterning and Size Control: Evidence and Genetically Encoded Tools in the Zebrafish Model

Silic

Zhang

2023

Cells

View full text Add to dashboard Cite

Developmental patterning is essential for regulating cellular events such as axial patterning, segmentation, tissue formation, and organ size determination during embryogenesis. Understanding the patterning mechanisms remains a central challenge and fundamental interest in developmental biology. Ion-channel-regulated bioelectric signals have emerged as a player of the patterning mechanism, which may interact with morphogens. Evidence from multiple model organisms reveals the roles of bioelectricity in embryonic development, regeneration, and cancers. The Zebrafish model is the second most used vertebrate model, next to the mouse model. The zebrafish model has great potential for elucidating the functions of bioelectricity due to many advantages such as external development, transparent early embryogenesis, and tractable genetics. Here, we review genetic evidence from zebrafish mutants with fin-size and pigment changes related to ion channels and bioelectricity. In addition, we review the cell membrane voltage reporting and chemogenetic tools that have already been used or have great potential to be implemented in zebrafish models. Finally, new perspectives and opportunities for bioelectricity research with zebrafish are discussed.

show abstract

“…In this type of configuration, the toxins are not added inside the pipette but in the surrounding cell media, instead (Figure 3B). This whole-cell patch clamp configuration has been used to evaluate the properties of TTX as a drug for the treatment of arrhythmias or other disorders [78]. The cell immobilization on the micropipette is a key factor, since a good seal is necessary to achieve an electrochemical signal.…”

Section: Cell-based Biosensorsmentioning

confidence: 99%

Detection of Ciguatoxins and Tetrodotoxins in Seafood with Biosensors and Other Smart Bioanalytical Systems

Reverté

Alkassar

Diogène

et al. 2023

Foods

View full text Add to dashboard Cite

The emergence of marine toxins such as ciguatoxins (CTXs) and tetrodotoxins (TTXs) in non-endemic regions may pose a serious food safety threat and public health concern if proper control measures are not applied. This article provides an overview of the main biorecognition molecules used for the detection of CTXs and TTXs and the different assay configurations and transduction strategies explored in the development of biosensors and other biotechnological tools for these marine toxins. The advantages and limitations of the systems based on cells, receptors, antibodies, and aptamers are described, and new challenges in marine toxin detection are identified. The validation of these smart bioanalytical systems through analysis of samples and comparison with other techniques is also rationally discussed. These tools have already been demonstrated to be useful in the detection and quantification of CTXs and TTXs, and are, therefore, highly promising for their implementation in research activities and monitoring programs.

show abstract

In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives

Cited by 28 publications

References 225 publications

The Neurovascular Unit Dysfunction in the Molecular Mechanisms of Epileptogenesis and Targeted Therapy

The Neurovascular Unit Dysfunction in the Molecular Mechanisms of Epileptogenesis and Targeted Therapy

Bioelectricity in Developmental Patterning and Size Control: Evidence and Genetically Encoded Tools in the Zebrafish Model

Detection of Ciguatoxins and Tetrodotoxins in Seafood with Biosensors and Other Smart Bioanalytical Systems

Contact Info

Product

Resources

About