In-vivo X-ray dark-field computed tomography for the detection of radiation-induced lung damage in mice

Burkhardt, Rico; Gora, Thomas; Fingerle, Alexander A.; Sauter, Andreas; Meurer, Felix; Gassert, Florian T.; Dobiasch, Sophie; Schilling, Daniela; Feuchtinger, Annette; Walch, Axel; Multhoff, Gabriele; Herzen, Julia; Noël, Peter B.; Rummeny, Ernst J.; Combs, Stephanie E.; Schmid, Thomas E.; Pfeiffer, Franz; Wilkens, Jan J.

doi:10.1016/j.phro.2021.09.003

Cited by 12 publications

(12 citation statements)

References 32 publications

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“…Mouse imaging for pathological and pharmacological research is widely employed before clinical applications of the relevant methods or medicines, such as detecting the radiation injury of murine lung through dark-field contrast (Burkhardt et al 2021, Gassert et al 2022, and studying emphysematous lung of mouse compared with histopathological slices (Velroyen et al 2015). We achieved comparable FOV and imaging performance at high energy, which can reduce the radiation dose and the metal artifacts.…”

Section: Conclusion and Discussionmentioning

confidence: 97%

High energy x-ray Talbot-Lau interferometer employing a microarray anode-structured target source to extend the field of view

Lin,

Wu,

Zan

et al. 2023

Phys. Med. Biol.

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Objective. High energy and large field of view (FOV) phase contrast imaging is crucial for biological and even medical applications. Although some works have devoted to achiving a large field of view at high energy through bending gratings and so on, which would be extremely challenging in medical high energy imaging. Approach. We analyze the angular shadowing effect of planar gratings in high-energy X-ray Talbot-Lau interferometer (XTLI). Then we design and develop an inverse XTLI coupled with a microarray anode-structured target source, to extend the FOV at high energy. Main results. Our experimental results demonstrate the benefit of the source in inverse XTLI and a large FOV of 106.6 mm in the horizontal direction is achieved at 40 keV. Based on this system, experiments of a mouse demonstrate the potential advantage of phase contrast mode in imaging lung tissue. Significance. We extend the FOV in a compact XTLI using a microarray anode-structured target source coupled with an inverse geometry, which eliminates grating G0 and relaxes the fabrication difficulty of G2. We believe the established design idea and imaging system would facilitate the wide applications of XTLI in high energy phase contrast imaging.

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Section: Conclusion and Discussionmentioning

confidence: 97%

High energy x-ray Talbot-Lau interferometer employing a microarray anode-structured target source to extend the field of view

Lin,

Wu,

Zan

et al. 2023

Phys. Med. Biol.

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show abstract

“…The most common 3D imaging modalities are computed tomography (CT) and magnetic resonance imaging (MRI) with superior soft-tissue contrast. For better differentiation and quantification of different tissue types, dual-and multi-energy CT were developed (McCollough et al 2015), complemented by novel x-ray modalities like phase contrast or dark-field imaging (Burkhardt et al 2021).…”

Section: Imaging Systems For Image Guidancementioning

confidence: 99%

“…Current and future challenges. Preclinical imaging uses a wide range of imaging modalities and even some that are not common in clinical practice such as ion radiography or CT, bioluminescent imaging, photo-acoustic imaging, phase-contrast x-ray imaging, or even dark x-ray imaging (Burkhardt et al 2021). Most of these imaging modalities may provide useful information for treatment planning e.g.…”

Section: Treatment Planningmentioning

confidence: 99%

Roadmap for precision preclinical x-ray radiation studies

et al. 2023

Self Cite

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This Roadmap paper covers the field of precision preclinical radiation studies in animal models. It is mostly focused on models for cancer and normal tissue response to radiation, but also discusses other disease models. The recent technological evolutions in imaging, irradiation, dosimetry and monitoring that have empowered these kinds of studies is discussed, and many developments in the near future are outlined. Finally, clinical translation and inverse translation are discussed.

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“…Especially, the scattering mode is sensitive to the pore structure of lung, and numerous papers have reported its possible advantage in lung disease diagnosis. 6,[19][20][21][22][23][24][25][26][27] The dark-field chest radiography is showed superior to conventional chest radiography in diagnosing and quantifying emphysema in living people 6 and mouse models. 19 Nelson et al quantified mice models of pulmonary fibrosis by complementing the x-ray dark-field and attenuationbased CT. 20 X-ray dark-field imaging is also used to assess and detect lung radiation damage 21 and pulmonary fibrosis.…”

Section: Introductionmentioning

confidence: 99%

Detection of early pulmonary emphysema by multi‐contrast x‐ray Talbot‐Lau interferometer

Lin,

Wu,

Huang

et al. 2024

Medical Physics

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BackgroundPulmonary emphysema is a part of chronic obstructive pulmonary disease, which is an irreversible chronic respiratory disease. In order to avoid further damage to lung tissue, early diagnosis and treatment of pulmonary emphysema is essential.PurposeEarly pulmonary emphysema diagnosis is difficult with conventional radiographic imaging. Recently, x‐ray phase contrast imaging has proved to be an effective and promising imaging strategy for soft tissue, due to its high sensitivity and multi‐contrast. The aim of this study is to diagnose pulmonary emphysema early utilizing an x‐ray Talbot‐Lau interferometer (TLI).MethodsWe successfully established the mouse model of emphysema by porcine pancreatic elastase treatment, and then used the established x‐ray TLI to perform imaging experiments on the mice with different treatment time. The traditional absorption CT and phase contrast CT were obtained simultaneously through TLI. The CT results and histopathology of mice lung in different treatment time were quantitatively analyzed.ResultsBy imaging mice lungs, it can be found that phase contrast has higher sensitivity than absorption contrast in early pulmonary emphysema. The results show that the phase contrast signal could distinguish the pulmonary emphysema earlier than the conventional attenuation signal, which can be consistent with histological images. Through the quantitative analysis of pathological section and phase contrast CT, it can be found that there is a strong linear correlation.ConclusionsIn this study, we quantitatively analyze mean linear intercept of histological sections and CT values of mice. The results show that the phase contrast signal has higher imaging sensitivity than the attenuation signal. X‐ray TLI multi‐contrast imaging is proved as a potential diagnostic method for early pulmonary emphysema in mice.

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In-vivo X-ray dark-field computed tomography for the detection of radiation-induced lung damage in mice

Abstract: Highlights Radiation-induced lung damage was observed using X-ray dark-field tomography. In this pre-clinical study, mouse lungs were irradiated and subsequently imaged. We report increased sensitivity of X-ray dark-field tomography over absorption-based tomography.

Cited by 12 publications

References 32 publications

High energy x-ray Talbot-Lau interferometer employing a microarray anode-structured target source to extend the field of view

High energy x-ray Talbot-Lau interferometer employing a microarray anode-structured target source to extend the field of view

Roadmap for precision preclinical x-ray radiation studies

Detection of early pulmonary emphysema by multi‐contrast x‐ray Talbot‐Lau interferometer

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