In VivoColored Microspheres in the Isolated Rat Heart for Use in NMR,

Hiller, K. H.; Adami, Peter; Voll, Sabine; Roder, Fridtjof; Kowallik, Peter; Bauer, Wolfgang R.; Haase, Axel; Ertl, Georg

doi:10.1006/jmcc.1996.0053

Cited by 16 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Colored microspheres have been used successfully in vivo and in the isolated rat heart (22)(23)(24). This method was used to validate the in vivo MR imaging perfusion technique.…”

Section: Reference Methods For Myocardial Perfusion: Colored Microsphementioning

confidence: 99%

Myocardial Perfusion and Intracapillary Blood Volume in Rats at Rest and with Coronary Dilatation: MR Imaging in Vivo with Use of a Spin-Labeling Technique

Waller

Kahler²,

Hiller³

et al. 2000

Radiology

101

View full text Add to dashboard Cite

show abstract

“…Colored microspheres have been used successfully in vivo and in the isolated rat heart (22)(23)(24). This method was used to validate the in vivo MR imaging perfusion technique.…”

Section: Reference Methods For Myocardial Perfusion: Colored Microsphementioning

confidence: 99%

Myocardial Perfusion and Intracapillary Blood Volume in Rats at Rest and with Coronary Dilatation: MR Imaging in Vivo with Use of a Spin-Labeling Technique

Waller

Kahler²,

Hiller³

et al. 2000

Radiology

101

View full text Add to dashboard Cite

show abstract

“…9,10 The number of microspheres (Dye-Trak; Triton Technology Inc; mean diameter 15 m) of 1 color was Ϸ30 000. Microspheres were flushed into the aorta with Krebs-Henseleit buffer containing 0.002% Tween 80 at 0.5 mL per minute over 10 minutes.…”

Section: Perfusion Measurement By Colored Microspheresmentioning

confidence: 99%

“…This is due to obvious technical restrictions of the preparation and to the fact that the microsphere technique demands more myocardial mass 9 than that of the imaging slice to provide reliable data. On the other hand, the close correlation of microsphere and NMR perfusion data suggests that the difference of the slices is of minor importance.…”

Section: Development Of Perfusion Imagingmentioning

confidence: 99%

Fast High-Resolution Magnetic Resonance Imaging Demonstrates Fractality of Myocardial Perfusion in Microscopic Dimensions

Bauer¹,

Hiller²,

Galuppo³

et al. 2001

Circulation Research

View full text Add to dashboard Cite

Abstract-The fractal nature of heterogeneity of myocardial blood flow and its implications for the healthy and diseased heart is not yet understood. The main hindrance for investigation of blood flow heterogeneity and its role in physiology and pathophysiology is that conventional methods for determination of myocardial perfusion have severe limitations concerning temporal and spatial resolution and invasiveness. In isolated rat hearts, we developed a nuclear magnetic resonance technique that does not depend on contrast agents and in which the apparent longitudinal relaxation time is made perfusion sensitive by selective preparation of the imaging slice. This perfusion-sensitive relaxation time is determined within 40 seconds as a map with a high spatial in-plane resolution of 140ϫ140 m 2 and a thickness of 1.5 mm. Perfusion imaging was validated with the established microsphere technique. Additionally, the congruence between perfusion-sensitive T 1 maps and first-pass perfusion imaging was demonstrated. As an application of high-resolution perfusion imaging, fractal analysis of the spatial distribution of perfusion was performed. We were able to demonstrate that the fractality of this distribution exists even in microscopic dimensions. Vasodilation by nitroglycerin modulated the fractal pattern of perfusion, and the decrease of the fractal dimension indicated a shift toward homogeneity. This implies that parameters of the fractal distribution depend on the microvascular tone rather than on anatomic preformations; ie, fractality is a functional characteristic of perfusion.

show abstract

“…Regional myocardial blood flow measurement using the CM method is a gold standard in animal experiments (20,(22)(23)(24). An accurate measurement of CO is necessary to determine absolute values of regional myocardial blood flow.…”

Section: Discussionmentioning

confidence: 99%

A Combined Method for the Determination of Myocardial Perfusion in Experimental Animals Using Microspheres and CMR

Surányi

Kiss

Símor

et al. 2007

J. of Cardiovascular Magnetic Resonance

View full text Add to dashboard Cite

A convenient method is introduced for myocardial perfusion research by combining multislice short-axis cine cardiovascular magnetic resonance (MSA-CMR) and colored microspheres (CM). In canine control-ischemia-reperfusion (n = 11), Cardiac output (CO) was measured using MSA-CMR (COMSA), Phase Contrast CMR (PC-CMR, COPC) and CM (from reference blood samples, COmicro) in 3 experimental periods per animal. COmicro significantly and systematically overestimated COMSA (median deviation: 291 mL/min, p < 0.01), while there was excellent agreement between PC-CMR and MSA-CMR without significant over-or underestimation (median deviation: -14 ml/min, p = NS). In quantitative myocardial perfusion assay by CM, CMR should be used instead of reference blood sampling. This should improve the accuracy of absolute regional perfusion measurement.

show abstract

In VivoColored Microspheres in the Isolated Rat Heart for Use in NMR,

Cited by 16 publications

References 0 publications

Myocardial Perfusion and Intracapillary Blood Volume in Rats at Rest and with Coronary Dilatation: MR Imaging in Vivo with Use of a Spin-Labeling Technique

Myocardial Perfusion and Intracapillary Blood Volume in Rats at Rest and with Coronary Dilatation: MR Imaging in Vivo with Use of a Spin-Labeling Technique

Fast High-Resolution Magnetic Resonance Imaging Demonstrates Fractality of Myocardial Perfusion in Microscopic Dimensions

A Combined Method for the Determination of Myocardial Perfusion in Experimental Animals Using Microspheres and CMR

Contact Info

Product

Resources

About