Inability of colchicine to inhibit newt epidermal cell migration or prevent concanavalin A-mediated inhibition of migration

Dunlap, Mary K.; Donaldson, Donald J.

doi:10.1016/0014-4827(78)90059-9

Cited by 25 publications

(9 citation statements)

References 19 publications

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“…Our dose-response curves for the effectiveness of colchicine in causing mitotic arrest in the regeneration blastema verify and explain the previous reports that low doses are often more effective than high doses (Dunlap and Donaldson 1978;Beach and Jacobson 1979). Thus, the assumption made by several researchers that a high dose of colchicine will result in more metaphase arrests than a low or zero dose is not justified (Donaldson and Wilson 1975;Mescher and Gospodarowicz 1979;Gospodarowicz and Mescher 1981).…”

Section: Discussionsupporting

confidence: 79%

“…Colchicine, a plant alkaloid, halts mitosis at metaphase by disrupting the assembly of microtubules so that the mitotic spindle cannot be formed (Eigsti and Dustin 1955;Borisy and Taylor 1967). However, there is considerable variation in the dose of colchicine used, the timing of the injection, and in the mitotic indices (number of mitotic figures/ number of nuclei counted) obtained (Donaldson and Wilson 1975;Dunlap and Donaldson 1978;Scadding and Vinette 1978;Beach and Jacobson 1979;Wertz and Donaldson 1980;Gospodarowicz and Mescher 198 1 ; 'present address: Department of Anatomy, University of Toronto, Toronto, Ont .…”

Section: Introductionmentioning

confidence: 95%

“…The results are confusing because when colchicine is injected, the maximum mitotic index is not always obtained with the highest dose. Dunlap and Donaldson (1978) used doses of 2 kg and 10 kg colchicine per gram body weight on newts and found that the lower dose collected more mitotic figures in epidermis than did the higher dose. S. R. Scadding and J. L. Vinette (unpublished observations) have made similar observations on Ambystoma larvae limb and tail regeneration blastemas.…”

Section: Introductionmentioning

confidence: 98%

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Limb regeneration blastema cells in the adult newt, Notophthalmus viridescens, give aberrant response to colchicine

Coomber¹,

Davidson²,

Scadding³

1983

Can. J. Zool.

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This study was designed to investigate the effect of colchicine on the mitotic index (number of mitotic figures per 1000 cells) in the newt limb regeneration blastema. Colchicine doses from 0.1 to 1000 μg per gram body weight and treatment durations from 2 to 72 h were used to determine the dose of colchicine and treatment period resulting in optimum metaphase arrest. A preliminary experiment demonstrated that there was no circadian rhythm of mitosis in the newt limb regeneration blastema. A dose of 5 μg colchicine per gram body weight was found to give the maximum mitotic index; both higher and lower doses had less effect. Very high doses of colchicine resulted in a mitotic index the same as that of the controls. The mitotic index increased with increasing treatment time up to 36 h, after which there was no further increase. In other newt tissues such as duodenal mucosa and liver, a more typical dose–response relationship was observed in that increased colchicine dose led to increased mitotic indices up to the level of lethal doses of colchicine.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 98%

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Limb regeneration blastema cells in the adult newt, Notophthalmus viridescens, give aberrant response to colchicine

Coomber¹,

Davidson²,

Scadding³

1983

Can. J. Zool.

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“…It was suggested that this func tion is carried out by the microtubular system [Bishey and Freed, 1971;Vasiliev and Gelfand, 1976]. How ever, in cells migrating in a cohesive form, as presum ably the precardiac cells do, these stabilizing effects are carried out by means of the firmess of local cell-cell contacts [Vasiliev and Gelfand, 1976;Dunlap and Donaldson, 1978]. Thus, microtubules do not appear to be necessary for the migration of the precardiac cells and, consequently, colchicine does not stop this active process.…”

Section: Discussionmentioning

confidence: 99%

Effects of Colchicine on the Formation and Looping of the Tubular Heart of the Embryonic Chick

Icardo¹,

Ojeda²

1984

Cells Tissues Organs

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The role of microtubules in the early development of the chick embryo heart was studied. The microtubules were disrupted by treatment of the embryos with colchicine. The embryos were divided for study into three groups: (I) before the fusion of the paired cardiac primordia; (II) before the starting of the cardiac loop, and (III) during the formation of the looping process. Colchicine did not impair the fusion of the paired heart primordia nor the acquisition of an asymmetric C-shaped form. However, the normal counterclockwise movement of the heart loop was prevented. From these results we conclude that the formation of the tubular heart and its looping are independent of the integrity of the microtubular system. Under the effects of colchicine the developing myocytes rounded up bulging into the pericardial cavity. The cell contours became scarcely discernible and the individual cell surfaces gave rise to blebs and ruffles of different sizes. In older embryos, clefts of different sizes appeared between the myocardial cells. The effects of colchicine on the pulsatile activity of the heart were recorded. These effects, as well as those on the cell surface characteristics, were found to be age dependent. The more mature the hearts were, the more resistant to colchicine they became. The developmental significance of the results reported here is discussed.

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“…Mitosis is not involved (Dunlap and Donaldson, 1978). During this process, basal and suprabasal cells move into the wound to create a one-to 0 1994 WILEY-LISS, INC.…”

mentioning

confidence: 98%

Migratory interaction of amphibian epidermal cells with components of the basement membrane

Donaldson

Mahan

Tsilibary

et al. 1994

Journal Cellular Physiology

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In adult newts, basal epidermal cells adjacent to a fresh wound move toward the damaged area by migrating over the epidermal basement membrane. In an attempt to determine which basement membrane components mediate this migration, small pieces of glass coated with various natural matrices, purified proteins, or fragments of proteins were implanted into skin wounds such that epidermal cells attempting to form a wound epithelium would encounter the implants. Laminin derived from a cell line (M1536-B3) that produces no type IV collagen was inactive as a migration substrate. Migration on recombinant entactin was somewhat better than on laminin but was still only approximately 14% of that on type I collagen. M15 matrix, a laminin and entactin-containing product of M1536-B3 cells, was no better than entactin alone. Type IV collagen was an excellent substrate, producing slightly more migration than corresponding concentrations of type I collagen at nearly all concentrations tested. Migration on type IV lacking the NC1 domain was at least as good as on intact type IV. All the activity in type IV was present in a 95 kD fragment (alpha 1(IV)95) from the carboxy terminal two-thirds of the alpha 1 chain. Approximately 60% of the activity on alpha 1(IV)95 was obtained on implants coated with a 110 amino acid fragment of the alpha 1 chain derived from the carboxy terminal half of alpha 1(IV)95. Adding the synthetic peptide, arg-gly-asp-ser (RGDS) to the medium, blocked migration on fibronectin-coated implants but had no effect on implants coated with type IV, suggesting that migration on type IV involves different cell surface receptors than those mediating migration over fibronectin. Matrigel, a commercial product containing most basement membrane components, was a poor migration substrate. Thus if type IV mediates basal cell migration toward a wound in vivo, there may have to be some alterations in basement membrane structure to allow epidermal receptors to access type IV active site(s).

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Inability of colchicine to inhibit newt epidermal cell migration or prevent concanavalin A-mediated inhibition of migration

Cited by 25 publications

References 19 publications

Limb regeneration blastema cells in the adult newt, Notophthalmus viridescens, give aberrant response to colchicine

Limb regeneration blastema cells in the adult newt, Notophthalmus viridescens, give aberrant response to colchicine

Effects of Colchicine on the Formation and Looping of the Tubular Heart of the Embryonic Chick

Migratory interaction of amphibian epidermal cells with components of the basement membrane

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