Inactivated Probiotic Bacteria Stimulate Cellular Immune Responses of Catla, Catla catla (Hamilton) In Vitro

Kamilya, Dibyendu; Baruah, Arunjyoti; Sangma, Timothy; Chowdhury, S.; Pal, Prasenjit

doi:10.1007/s12602-015-9191-9

Cited by 44 publications

(12 citation statements)

References 23 publications

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“…The study results also showed an increased respiratory burst activity in para-probiotic treatments that were significantly higher than control treatments, mainly in the P10 treatment. These results were also similar to Kamilya et al (2015), that the inactivated-Bacillus subtilis FPTB13 supplementation could induce the respiratory burst activity in Rohu carp. Dash et al (2015) also reported that respiratory burst activity of giant freshwater prawn (Macrobrachium rosenbergii) increased significantly, after inactivated-L. plantarum supplementation.…”

Section: Discussionsupporting

confidence: 89%

The effects of different dosage applications of Bacillus sp. NP5 paraprobiotic on the growth performance and resistance of Nile tilapia against Streptococcus agalactiae infectio

Widanarni

Sukenda

Anggraeni

et al. 2022

Jurnal Akuakultur Indonesia

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The aim of the study is to test the effectiveness of adding various paraprobiotic doses of Bacillus sp. NP5 through feed in improving the immune responses and resistance of tilapia juvenile (Oreochromis niloticus) to infection of Streptococcus agalactiae. Bacillus sp. NP5 paraprobiotic was produced through heat-inactivation at 95°C for 1 h. This study used a completely randomized design, which consisted of four treatments with three repetitions. The test feed was enriched with 1% paraprobiotic with a density of 108, 109, and 1010 CFU ml-1. The tilapia juvenile (10.29 ± 0.22 g) were reared for 30 days and on day 31, the fish was challenge tested with S. agalactiae (107 CFU ml-1) injected by intraperitoneal route unless the negative control was injected with PBS. The results of this study showed significantly improved growth performances and immune responses (P<0.05), compared to the control group after 30 days of paraprobiotic Bacillus sp. NP5 administered. The increased of immune responses in paraprobiotic of Bacillus sp. NP5 showed a higher survival rate (P<0.05), than positive control after the challenge tested with S. agalactiae. Administration of paraprobiotic Bacillus sp. NP5 through the commercial feed able to improve the growth performances, immune responses, and resistance of nile tilapia against S. agalactiae infection with the best result at dose of 1010 CFU ml-1.

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Section: Discussionsupporting

confidence: 89%

The effects of different dosage applications of Bacillus sp. NP5 paraprobiotic on the growth performance and resistance of Nile tilapia against Streptococcus agalactiae infectio

Widanarni

Sukenda

Anggraeni

et al. 2022

Jurnal Akuakultur Indonesia

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“…, ; Kamilya et al . ; Singh et al . ), keeping probiotic bacteria in boiling water for 1–2 h (Villamil et al .…”

Section: Inactivation Methodsmentioning

confidence: 99%

“…; Kamilya et al . ). Up‐regulated in vitro expression of many immune‐relevant genes including pro‐inflammatory cytokines [IL‐1 (IL‐1β), IL‐6, IL‐8, IL‐17A/F‐3, TNF‐ (TNF‐a and TNF‐N) and COX‐2], cell‐mediated immune regulators [IL‐12p35, IL‐12p40 and IL‐18], antiviral cytokines [I‐IFN and IFN‐γ], other regulatory cytokines [IL‐2, IL‐7, IL‐15, IL‐21, IL‐10 and TGF‐β1], antibacterial epinecidin‐1, IgM and TLR2 have also been reported (Biswas et al .…”

Section: Paraprobiotic Application In Aquaculturementioning

confidence: 97%

Paraprobiotics: an aquaculture perspective

Choudhury

Kamilya

2018

Reviews in Aquaculture

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Probiotics, which are considered as live microbial adjunct conferring health benefits to the host, represent a potentially effective strategy for infectious disease control. Nevertheless, there has been growing concerns about safety, applicability and functionality issues associated with the live probiotic microorganisms. Concurrently, recent observations indicate that even the non-viable counterparts of the probiotic organisms, also known as paraprobiotic, can be used to harness similar beneficial effects. Considering the limitations of probiotic application, paraprobiotics constitute a potential alternative strategy for improving health and wellness of fish and shellfish in aquaculture. Eventhough the concept of paraprobiotic is relatively established in higher vertebrate models and related food production sectors, its application in aquaculture is still in its early stage. In light of this, the present review delineates the concept of paraprobiotic, inactivation methods used for its preparation, the role of paraprobiotic in modifying biological responses as well as their potential application in aquaculture.

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“…Postbiotic Lactobacillus casei Zhang (LcZ) (heat inactivated and suspended at 10 6 cfu/mL in PBS) enhances the expression of proinflammatory cytokines and the transcription of TLR2, TLR3, TLR4, and TLR9, thus increasing the macrophage-mediated innate immune response [166]. As a result of the study conducted with the live and inactive forms of Bacillus amyloliquefaciens FPTB16 and Bacillus subtilis FPTB13, it was reported that the inactive preparation increased the secretion of cellular immune parameters more than the live preparation [167]. In addition, a study in mice reported that the combination of heat-inactivated (two heat treatments were applied: 30 min at 100 • C and 15 min at 121 • C) LAB increased immunomodulatory activity in macrophages more than the same combination (Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus fermentum, and Enterococcus faecium) containing live strains [168].…”

Section: Postbiotics and Immune Systemmentioning

confidence: 99%

Involvement of Probiotics and Postbiotics in the Immune System Modulation

et al. 2021

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Intestinal microbiota interacts with other systems, especially the immune system, which is responsible for protecting the body by recognizing “stranger” (pathogen associated molecular patterns-PAMPs) and “danger” (damage-associated molecular patterns-DAMPs) molecular motifs. In this manner, it plays an important role in the pathogenesis of various diseases and health. Despite the use of probiotics that modulate the intestinal microbiota in providing health benefits and in the treatment of diseases, there are some possible concerns about the possibility of developing adverse effects, especially in people with suppressed immune systems. Since probiotics provide health benefits with bioactive compounds, studies are carried out on the use of products containing non-living probiotic microorganisms (paraprobiotics) and/or their metabolites (postbiotics) instead of probiotic products. It is even reported that these microbial compounds have more immunomodulatory activities than living microorganisms via some possible mechanism and eliminates some disadvantages of probiotics. Considering the increasing use of functional foods in health and disease, further studies are needed with respect to the benefits and advantages of parabiotic and/or postbiotic use in the food and pharmaceutical industry as well as immune system modulation. Although probiotics have been extensive studied for a long time, it seems that postbiotics are promising tools for future research and applications according to the recent literature. This review aimed to evaluate the interaction of probiotics and postbiotics with the immune systems and also their advantages and disadvantages in the area of food-pharmaceutical industry and immune system modulation.

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Inactivated Probiotic Bacteria Stimulate Cellular Immune Responses of Catla, Catla catla (Hamilton) In Vitro

Cited by 44 publications

References 23 publications

The effects of different dosage applications of Bacillus sp. NP5 paraprobiotic on the growth performance and resistance of Nile tilapia against Streptococcus agalactiae infectio

The effects of different dosage applications of Bacillus sp. NP5 paraprobiotic on the growth performance and resistance of Nile tilapia against Streptococcus agalactiae infectio

Paraprobiotics: an aquaculture perspective

Involvement of Probiotics and Postbiotics in the Immune System Modulation

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