Inactivated whole-cell bacterial vaccines: current status and novel strategies

Pace, John; Rossi, Humberto A.; Esposito, Vito M.; Frey, Steve M.; Tucker, Kenneth D.; Walker, Richard I.

doi:10.1016/s0264-410x(98)00046-2

Cited by 34 publications

(22 citation statements)

References 130 publications

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“…This method of immunization is not strange, as previous vaccinations with whole bacteria by oral injection, as well as oral immunizations with BCG, have been administered (23,24). However, this might be the first time that a nanoparticle form of chitosan was used to deliver the ompW protein in an animal model to induce an immune response.…”

Section: Discussionmentioning

confidence: 99%

Serum Anti-Vibrio cholerae Immunoglobulin Isotype in BALB/c Mice Immunized With ompW-Loaded Chitosan

Fasihi-Ramandi¹,

Ghobadi-Ghadikolaee²,

Ahmadi-Renani³

et al. 2016

Int J Enteric Pathog

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Section: Discussionmentioning

confidence: 99%

Serum Anti-Vibrio cholerae Immunoglobulin Isotype in BALB/c Mice Immunized With ompW-Loaded Chitosan

Fasihi-Ramandi¹,

Ghobadi-Ghadikolaee²,

Ahmadi-Renani³

et al. 2016

Int J Enteric Pathog

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“…[7][8][9] This is the case for microorganisms such as bacteria and viruses. [10,11] Conversely, small and soluble antigens, such as proteins and peptides, are poorly internalized and thus weakly immunogenic; therefore, an adjuvant is needed to intensify the immune response. [12,13] Triggering an efficient immune response to low-molecular-weight compounds 60 (haptens) is an even more challenging task because they are smaller and lack a peptide structure, so they cannot be presented by MHC II molecules unless they are conjugated to proteins prior to immunization.…”

Section: Introductionmentioning

confidence: 99%

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Parra

Abad‐Somovilla

Mercader

et al. 2013

Journal of Controlled Release

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Carbon nanotubes (CNTs) are nanomaterials with interesting emerging applications. Their 25 properties makes CNTs excellent candidates for use as new nanovehicles in drug delivery, immunization and diagnostics. In the current study, we assessed the immune-response-amplifying properties of CNTs to haptens by using azoxystrobin, the first developed strobilurin fungicide, as a model analyte. An azoxystrobin derivative bearing a carboxylated spacer arm (hapten AZc6) was covalently coupled to bovine serum albumin (BSA), and the resulting BSA-AZc6 conjugate was 30 covalently linked to four functionalized CNTs of different shapes and sizes, varying in diameter and length. These four types of CNT-based constructs were obtained using efficient, fast, and easy functionalization procedures based on microwave-assisted chemistry. New Zealand rabbits and BALB/c mice were immunized with BSA-AZc6 alone and with the four CNT-BSA-AZc6 constructs, both with and without Freund's adjuvant. The IgG-type antibody responses were assessed 35 in terms of the titer and affinity, paying special attention to the relationship between the immune response and the size and shape of the employed CNTs. Immunization with CNT-BSA-AZc6 resulted in enhanced titers and excellent affinities for azoxystrobin. More important, remarkable IgG responses were obtained even in the absence of an adjuvant, thus proving the self-adjuvanting capability of CNTs. Immunogens were able to produce strong anti-azoxystrobin immune responses 40 in rabbits even when administered at a BSA-AZc6 conjugate dose as low as 0.05 g. The short and thick CNT-BSA-AZc6 construct produced the best antibody response under all tested conditions.

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“…Strategies for producing traditional killed vaccines involve the use of heat or chemical treatment to inactivate bacterial cells; however, these strategies can affect the physiochemical/structural properties of bacterial surface antigens and thus potentially inhibit the development of protective immunity (6). Traditional killed vaccines generally require a strong chemical adjuvant and several injections to induce suitable immunity, and they pose a greater risk of allergic reactions and vaccine injection site sarcomas (7).…”

mentioning

confidence: 99%

Salmonella enterica Serovar Enteritidis Ghosts Carrying the Escherichia coli Heat-Labile Enterotoxin B Subunit Are Capable of Inducing Enhanced Protective Immune Responses

Jawale

Lee

2014

Clin Vaccine Immunol

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The Escherichia coli heat-labile enterotoxin B subunit (LTB) is a potent vaccine adjuvant. Salmonella enterica serovar Enteritidis ghosts carrying LTB (S. Enteritidis-LTB ghosts) were genetically constructed using a novel plasmid, pJHL187-LTB, designed for the coexpression of the LTB and E lysis proteins. S. Enteritidis-LTB ghosts were characterized using scanning electron microscopy to visualize their transmembrane tunnel structures. The expression of LTB in S. Enteritidis-LTB ghost preparations was confirmed by immunoblot and enzyme-linked immunosorbent assays. The parenteral adjuvant activity of LTB was demonstrated by immunizing chickens with either S. Enteritidis-LTB ghosts or S. Enteritidis ghosts. Chickens were intramuscularly primed at 5 weeks of age and subsequently boosted at 8 weeks of age. In total, 60 chickens were equally divided into three groups

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Inactivated whole-cell bacterial vaccines: current status and novel strategies

Cited by 34 publications

References 130 publications

Serum Anti-Vibrio cholerae Immunoglobulin Isotype in BALB/c Mice Immunized With ompW-Loaded Chitosan

Serum Anti-Vibrio cholerae Immunoglobulin Isotype in BALB/c Mice Immunized With ompW-Loaded Chitosan

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Salmonella enterica Serovar Enteritidis Ghosts Carrying the Escherichia coli Heat-Labile Enterotoxin B Subunit Are Capable of Inducing Enhanced Protective Immune Responses

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