Inactivation Effects of Non‐Thermal Atmospheric‐Pressure Helium Plasma Jet on <i>Staphylococcus aureus</i> Biofilms

Xu, Zimu; Shen, Jie; Zhang, Zelong; Ma, Jie; Ma, Ronghua; Zhao, Ying; Sun, Qibin; Qian, Shulou; Zhang, Hao; Ding, Lili; Cheng, Cheng; Chu, Paul K.; Xia, Weidong

doi:10.1002/ppap.201500006

Cited by 68 publications

(40 citation statements)

References 57 publications

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“…Bacteria produce carbohydrate matrices and may therefore reduce the effectiveness of decontamination methods (Xu et al . ). If a bacterium survives, it can exist in a VBNC state.…”

Section: Stress Environments Parameters and Possible Recovery (Vbnc mentioning

confidence: 97%

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State of the art of nonthermal and thermal processing for inactivation of micro-organisms

et al. 2018

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Despite the constant development of novel thermal and nonthermal technologies, knowledge on the mechanisms of microbial inactivation is still very limited. Technologies such as high pressure, ultraviolet light, pulsed light, ozone, power ultrasound and cold plasma (advanced oxidation processes) have shown promising results for inactivation of micro-organisms. The efficacy of inactivation is greatly enhanced by combination of conventional (thermal) with nonthermal, or nonthermal with another nonthermal technique. The key advantages offered by nonthermal processes in combination with sublethal mild temperature (<60°C) can inactivate micro-organisms synergistically. Microbial cells, when subjected to environmental stress, can be either injured or killed. In some cases, cells are believed to be inactivated, but may only be sublethally injured leading to their recovery or, if the injury is lethal, to cell death. It is of major concern when micro-organisms adapt to stress during processing. If the cells adapt to a certain stress, it is associated with enhanced protection against other subsequent stresses. One of the most striking problems during inactivation of micro-organisms is spores. They are the most resistant form of microbial cells and relatively difficult to inactivate by common inactivation techniques, including heat sterilization, radiation, oxidizing agents and various chemicals. Various novel nonthermal processing technologies, alone or in combination, have shown potential for vegetative cells and spores inactivation. Predictive microbiology can be used to focus on the quantitative description of the microbial behaviour in food products, for a given set of environmental conditions.

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“…Bacteria produce carbohydrate matrices and may therefore reduce the effectiveness of decontamination methods (Xu et al . ). If a bacterium survives, it can exist in a VBNC state.…”

Section: Stress Environments Parameters and Possible Recovery (Vbnc mentioning

confidence: 97%

“…In order to survive micro-organisms tend to form biofilms, the most powerful microbial defensive system. Bacteria produce carbohydrate matrices and may therefore reduce the effectiveness of decontamination methods (Xu et al 2015). If a bacterium survives, it can exist in a VBNC state.…”

Section: Stress Environments Parameters and Possible Recovery (Vbnc mentioning

confidence: 99%

State of the art of nonthermal and thermal processing for inactivation of micro-organisms

et al. 2018

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“…The intracellular ROS production in biofilm Staphylococcus aureus upon treatment using helium (He) plasma was observed by Xu et al . (). They reported that ROS plays pivotal and synergistic roles in the inactivation of microbial biofilms in conjunction with direct etching effect.…”

Section: Plasma Species and Their Role In Inactivationmentioning

confidence: 97%

“…The He/O 2 plasma microjet was reported to produce reactive oxygen species (ROS) such as hydroxyl radical ( • OH), superoxide anion radical ( • O 2 À ) and singlet molecular oxygen ( 1 O 2 ) (Sun et al 2012). The intracellular ROS production in biofilm Staphylococcus aureus upon treatment using helium (He) plasma was observed by Xu et al (2015). They reported that ROS plays pivotal and synergistic roles in the inactivation of microbial biofilms in conjunction with direct etching effect.…”

Section: Gas Discharge Plasmasmentioning

confidence: 99%

“…Under optimal conditions, bacterial cells in Staph. aureus biofilms were killed (>99Á9%) by the plasmas within 10 min of exposure and no bacteria nor biofilm regrowth from the plasma-treated biofilms was observed for 150 h. Xu et al (2015) investigated the antimicrobial effects of helium atmospheric pressure plasma jet against Staph. aureus biofilms in vitro.…”

Section: Opportunistic Pathogen Staphylococcus Aureusmentioning

confidence: 99%

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Potential applications of nonthermal plasmas against biofilm-associated micro-organisms in vitro

Puligundla

Mok

2017

J Appl Microbiol

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Summary Biofilms as complex microbial communities attached to surfaces pose several challenges in different sectors, ranging from food and healthcare to desalination and power generation. The biofilm mode of growth allows microorganisms to survive in hostile environments and biofilm cells exhibit distinct physiology and behaviour in comparison with their planktonic counterparts. They are ubiquitous, resilient and difficult to eradicate due to their resistant phenotype. Several chemical‐based cleaning and disinfection regimens are conventionally used against biofilm‐dwelling micro‐organisms in vitro. Although such approaches are generally considered to be effective, they may contribute to the dissemination of antimicrobial resistance and environmental pollution. Consequently, advanced green technologies for biofilm control are constantly emerging. Disinfection using nonthermal plasmas (NTPs) is one of the novel strategies having a great potential for control of biofilms of a broad spectrum of micro‐organisms. This review discusses several aspects related to the inactivation of biofilm‐associated bacteria and fungi by different types of NTPs under in vitro conditions. A brief introduction summarizes prevailing methods in biofilm inactivation, followed by introduction to gas discharge plasmas, active plasma species and their inactivating mechanism. Subsequently, significance and aspects of NTP inactivation of biofilm‐associated bacteria, especially those of medical importance, including opportunistic pathogens, oral pathogenic bacteria, foodborne pathogens and implant bacteria, are discussed. The remainder of the review discusses majorly about the synergistic effect of NTPs and their activity against biofilm‐associated fungi, especially Candida species.

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Microbial Inactivation with Heat Treatments

Titikshya

Sahoo

Kumar

et al. 2022

Thermal Food Engineering Operations

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Inactivation Effects of Non‐Thermal Atmospheric‐Pressure Helium Plasma Jet on Staphylococcus aureus Biofilms

Cited by 68 publications

References 57 publications

State of the art of nonthermal and thermal processing for inactivation of micro-organisms

State of the art of nonthermal and thermal processing for inactivation of micro-organisms

Potential applications of nonthermal plasmas against biofilm-associated micro-organisms in vitro

Microbial Inactivation with Heat Treatments

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