Inactivation efficiency of Escherichia coli and autochthonous bacteria during ozonation of municipal wastewater effluents quantified with flow cytometry and adenosine tri-phosphate analyses

Lee, Yunho; Imminger, Stefanie; Czekalski, Nadine; Gunten, Urs von; Hammes, Frederik

doi:10.1016/j.watres.2016.05.089

Cited by 77 publications

(52 citation statements)

References 40 publications

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“…Previous ozone disinfection studies which were performed in clean water matrices have determined the inactivation kinetic parameters for those laboratory-cultured indicator microorganisms, such as E. coli, Bacillus subtilis spores, and Cryptosporidium parvum oocysts, suggesting that ozone has inherently high reactivity with most microorganisms (Driedger et al, 2001;Finch et al, 1993;von Gunten, 2003b). As to secondary wastewater effluents containing high concentrations of particles, and dissolved organic carbon (DOC), a few recent studies have shown that ozonation can also achieve significant inactivation of indicator bacteria and various autochthonous bacterial communities at economically affordable O 3 doses, e.g., O 3 /DOC mass ratios of 0.5-1.0 Gamage et al, 2013;Lee et al, 2016). In recent years, antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) were increasingly detected in various environmental sources (Martinez, 2008).…”

Section: ; Vonmentioning

confidence: 99%

“…The FCM analysis combined with fluorescence staining pretreatment can be used for a range of qualitative and quantitative analyses of the microbial community including total cell count, size estimation, cell function, community structure and specific detection (Wang et al, 2010). For the assessment of bacterial viability during water and wastewater disinfection, the FCM provides a rapid and comprehensive alternative to cultivation-based methods Hammes et al, 2008;Lee et al, 2016;Phe et al, 2005;Van Nevel et al, 2017).…”

Section: ; Vonmentioning

confidence: 99%

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Applying UV absorbance and fluorescence indices to estimate inactivation of bacteria and formation of bromate during ozonation of water and wastewater effluent

Cheng

Cai

et al. 2018

Water Research

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Ozone is an effective oxidant and disinfectant commonly used for elimination of micropollutants and inactivation of resistant microbes. However, undesirable oxidation/disinfection byproducts such as bromate might form during ozonation. In this study, the UV absorbance and fluorescence indices were applied as surrogate indicators for predicting the inactivation of bacteria and formation of bromate during ozonation of water and wastewater effluents. The inactivation efficiencies of lab-cultured Escherichia coli (E. coli) and autochthonous bacteria were measured by plating (for E. coli only) and flow cytometry with fluorescence staining. During ozonation of E. coli spiked into wastewater effluents (∼10 cell/mL), the priority of inactivation efficiency determined by different cell viability methods were in the order of CFU > membrane damage > DNA damage. Approximately, 99% membrane damage and/or 90% DNA damage are conservatively supposed as an indicator for sufficient bacterial inactivation as well as degradation of antibiotic resistance genes. The related required O dosing thresholds for sufficient inactivation of E. coli and autochthonous bacteria refer to ∼0.6 O/DOC (g/g), ∼50% decrease of UVA254, ∼60% decrease of UVA280, or ∼80% decrease of humic-like fluorescence. Within the range of 10-10 cell/mL, the bacterial concentration did not have significant effects on the required thresholds of the specific O doses or spectroscopic indicators required for bacterial inactivation. The addition of 50 mM tert-BuOH as ·OH scavenger increased the required specific ozone doses but decreased the losses of spectroscopic indicators necessary for sufficient bacterial inactivation, and also suggested that the membrane/DNA damages for bacterial inactivation were mainly attributed to the direct O attacks. The bromate concentration was determined using ion chromatography with MS/MS detection. The results showed that when O was dosed at the required thresholds for sufficient bacterial inactivation, bromate formation could usually be suppressed below 10 μg/L. The present work supports that it is possible to reach a balance between bacterial inactivation and bromate formation.

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Section: ; Vonmentioning

confidence: 99%

Section: ; Vonmentioning

confidence: 99%

Applying UV absorbance and fluorescence indices to estimate inactivation of bacteria and formation of bromate during ozonation of water and wastewater effluent

Cheng

Cai

et al. 2018

Water Research

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“…Physical methods using UV light, ultrasound and plasma show poor efficiencies . In chemical methods, oxidants such as chlorine, chloramine, chlorine dioxide, and ozone (O 3 ) are employed . Among them, ozone is the strongest disinfectant and generates much less toxic disinfection by‐products (DBPs) compared with the widely used chlorination.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3] In chemical methods, oxidants such as chlorine, chloramine, chlorine dioxide, and ozone (O 3 ) are employed. [4][5][6] Among them, ozone is the strongest disinfectant and generates much less toxic disinfection by-products (DBPs) compared with the widely used chlorination. However, the low solubility of O 3 in water restricts its presence in the liquid phase, resulting in limited oxidation efficiency and O 3 wastage.…”

Section: Introductionmentioning

confidence: 99%

Ozonation inactivation of Escherichia coli in aqueous solution over MgO nanocrystals: modelling and mechanism

Yang

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND This study aims to reveal the potential of using heterogeneous catalytic ozonation processes (HCOP) in water disinfection and provide a direction to tailor the catalytic activity by crystal facet engineering. Four kinds of MgO nanocrystals individually with exposure of (111), (200), (110) and (100) facets were prepared and used. RESULTS It is found that MgO functions as a bactericide as well as a catalyst for ozonation. The catalytic activity of MgO nanocrystals decreases in the order MgO(111) > MgO(200) > MgO(110) > MgO(100). The apparent first‐order inactivation rate (kap) fitted by the Hom model of MgO(111)/O3 is 2.56 min‐1, which is 32.0 and 3.5 times that of single ozonation and classic MgO(100)/O3. It is confirmed by scavenger studies that •OH mainly works in MgO(111)/O3 while more direct ozonation occurs in other cases. Some O2•− are also detected in the presence of MgO. With the attack of these reactive oxidation species, cell membranes were damaged and intracellular components were released, leading to the death of Escherichia coli. CONCLUSION These results indicate that HCOP is a promising technology for water disinfection, and crystal facet engineering is an effective strategy to enhance the catalytic performance. © 2017 Society of Chemical Industry

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“…Ozone. Lee, Imminger, Czekalski, Von Gunten and Hammes (2016) studied inactivation kinetics of autochthonous bacteria during ozonation of wastewater effluents and found that intracellular adenosine triphosphate (ATP) and intact cell count (ICC) decreased with increasing ozone doses, with ICC being the more conservative parameter. The flow cytometry data showed that autochthonous bacteria were composed of communities with high and low ozone reactivity.…”

mentioning

confidence: 99%

Disinfection Processes

Kuo¹

2017

Water Environment Research

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Inactivation efficiency of Escherichia coli and autochthonous bacteria during ozonation of municipal wastewater effluents quantified with flow cytometry and adenosine tri-phosphate analyses

Cited by 77 publications

References 40 publications

Applying UV absorbance and fluorescence indices to estimate inactivation of bacteria and formation of bromate during ozonation of water and wastewater effluent

Applying UV absorbance and fluorescence indices to estimate inactivation of bacteria and formation of bromate during ozonation of water and wastewater effluent

Ozonation inactivation of Escherichia coli in aqueous solution over MgO nanocrystals: modelling and mechanism

Disinfection Processes

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