Inactivation Kinetics of Model and Relevant Blood-borne Viruses by Treatment with Sodium Hydroxide and Heat

Borovec, Steven; Broumis, Connie; Adcock, Wayne; Fang, Randel; Uren, Eric

doi:10.1006/biol.1998.0146

Cited by 28 publications

(22 citation statements)

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“…Indeed, very significant differences in the resistance to heat of viruses potentially present in sludge have been reported (8,22,29). The comparison of the levels of resistance of enteroviruses and phages reported here, a comparison of the levels of resistance of enteroviruses and parvoviruses with the level of resistance of phage f2, an F-specific RNA phage (29), and a comparison of the levels of resistance of phages MS2 and X174 with the level of resistance of hepatitis A virus (22) suggest that most human viruses are more readily inactivated by heat treatment than naturally occurring somatic coliphages and phages infecting B. fragilis are.…”

Section: Discussionmentioning

confidence: 99%

Survival of Bacterial Indicator Species and Bacteriophages after Thermal Treatment of Sludge and Sewage

Mocé-Llivina

Muniesa

Pimenta-Vale

et al. 2003

Appl Environ Microbiol

139

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The inactivation of naturally occurring bacterial indicators and bacteriophages by thermal treatment of a dewatered sludge and raw sewage was studied. The sludge was heated at 80°C, and the sewage was heated at 60°C. In both cases phages were significantly more resistant to thermal inactivation than bacterial indicators, with the exception of spores of sulfite-reducing clostridia. Somatic coliphages and phages infecting Bacteroides fragilis were significantly more resistant than F-specific RNA phages. Similar trends were observed in sludge and sewage. The effects of thermal treatment on various phages belonging to the three groups mentioned above and on various enteroviruses added to sewage were also studied. The results revealed that the variability in the resistance of phages agreed with the data obtained with the naturally occurring populations and that the phages that were studied were more resistant to heat treatment than the enteroviruses that were studied. The phages survived significantly better than Salmonella choleraesuis, and the extents of inactivation indicated that naturally occurring bacteriophages can be used to monitor the inactivation of Escherichia coli and Salmonella.

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Section: Discussionmentioning

confidence: 99%

Survival of Bacterial Indicator Species and Bacteriophages after Thermal Treatment of Sludge and Sewage

Mocé-Llivina

Muniesa

Pimenta-Vale

et al. 2003

Appl Environ Microbiol

139

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“…Viral preparations from EBV-positive Akata cells stimulated with anti-immunoglobulin G (anti-IgG) antibodies, IgG-stimulated EBV-negative Akata cells (mock controls), EBV-positive AGS cells stimulated with PMA, and PMA-stimulated EBV-negative AGS cells (mock controls) were produced as described previously (9,24). In selected experiments, EBV virions were inactivated by heating (1 h, 60°C) (8,36) or by exposure to UV (1 h, 265 nm) (3) prior to cell treatment. The specificity of the EBV response was evaluated by pretreatment of viral preparations with the neutralizing monoclonal antibody 72A1 (ATCC, Manassas, VA) raised against the viral envelope gp350/220 of EBV as described previously (23).…”

Section: Methodsmentioning

confidence: 99%

Epstein-Barr Virus Induces MCP-1 Secretion by Human Monocytes via TLR2

Gaudreault

Fiola

Olivier

et al. 2007

J Virol

137

146

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“…Boschetti et al showed 4.7 log reduction of Minute Virus of Mice after 1 min of exposure to 0.1 M sodium hydroxide. Previous studies have shown that sodium hydroxide at 60°C resulted in approximately a 5 log reduction in human hepatitis A virus infectivity after 16 min of treatment (Borovec et al, 1998). Additionally, literature indicates that a 3-5 log reduction in adenovirus type 3 infectivity is obtained following treatment with calcium hydroxide over a period of many hours at room temperature (Derbyshire and Brown, 1979).…”

Section: Introductionmentioning

confidence: 99%

“…Caustic solutions have previously been recommended for reducing the infectivity of viruses and other pathogenic organisms (Borovec et al, 1998;Boschetti et al, 2003;Derbyshire and Arkell, 1971;Lancz, 1976;Ye et al, 2003). Boschetti et al showed 4.7 log reduction of Minute Virus of Mice after 1 min of exposure to 0.1 M sodium hydroxide.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Adenovirus Type 5 by Caustics

Jannat

Hsu

Maheshwari

2008

Biotechnol Progress

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Adenovirus shows significant promise as a vehicle for transfer of therapeutic genes into humans. Based on the importance of this viral vector, it is critical that adequate decontamination procedures are implemented during its large-scale production in multiproduct manufacturing facilities to prevent cross-product contamination and to reduce the risk of personnel exposure. Liquid decontamination procedures based on caustics are easily implemented in a manufacturing setting and are not corrosive to stainless steel surfaces at the concentrations found to inactivate viral proteins and nucleic acids. In this study, we have conducted small-scale experiments to determine the effectiveness of caustic inactivation procedures on adenovirus type 5 and have evaluated the robustness of the process to different sample matrices and adenovirus constructs. We find that the pH of a sample post-addition of caustic solution is a more accurate indicator of the effectiveness of the caustic than its concentration. We have demonstrated that a greater than 6 log reduction in the potency of adenovirus type 5 may be obtained upon exposure of the sample to sodium hydroxide and CIP-100 at concentrations greater than 0.09 M and 0.9%, respectively, at times greater than 10 min.

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Inactivation Kinetics of Model and Relevant Blood-borne Viruses by Treatment with Sodium Hydroxide and Heat

Cited by 28 publications

References 0 publications

Survival of Bacterial Indicator Species and Bacteriophages after Thermal Treatment of Sludge and Sewage

Survival of Bacterial Indicator Species and Bacteriophages after Thermal Treatment of Sludge and Sewage

Epstein-Barr Virus Induces MCP-1 Secretion by Human Monocytes via TLR2

Inactivation of Adenovirus Type 5 by Caustics

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