Inactivation of aldehyde dehydrogenase: A key factor for engineering 1,3-propanediol production by Klebsiella pneumoniae

Zhang, Yanping; Li, Yin; Du, Chenyu; Liu, Ming; Zhu-an, Cao

doi:10.1016/j.ymben.2006.05.008

Cited by 121 publications

(75 citation statements)

References 21 publications

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“…The formation of lactate and ethanol compete with the formation of 1,3-PDO through the consumption of reducing equivalents, and often impose difficulties on the downstream processes responsible for the purification and recovery of 1,3-PDO [35,36]. By creating a lactate deficient mutant (LDH3) from K. oxytoca M5a1, the productivity and yield of 1,3-PDO were increased from 0.63 to 0.83 g/L/h to 0.41 to 0.53 mol/mol glycerol, respectively [35].…”

Section: Klebsiella Speciesmentioning

confidence: 99%

“…By creating a lactate deficient mutant (LDH3) from K. oxytoca M5a1, the productivity and yield of 1,3-PDO were increased from 0.63 to 0.83 g/L/h to 0.41 to 0.53 mol/mol glycerol, respectively [35]. By inactivating aldehyde dehydrogenase (ADH), the enzyme responsible for ethanol formation in K. pneumoniae YMU2, less ethanol was produced in the broth (from 9.26 g/L to 1.70 g/L) with a higher productivity (from 0.81 g/L/h to 1.07 g/L/h) and yield (from 0.36 mol/mol to 0.70 mol/mol) of 1,3-PDO observed [36]. To enhance both glycerol oxidative and reductive metabolisms, formate dehydrogenase (FDH) from Candida boidinii was expressed in K. oxytoca YMU1 [89].…”

Section: Klebsiella Speciesmentioning

confidence: 99%

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Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products

Lesnik

Liang

2013

Energies

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Biodiesel has gained a significant amount of attention over the past decade as an environmentally friendly fuel that is capable of being utilized by a conventional diesel engine. However, the biodiesel production process generates glycerol-containing waste streams which have become a disposal issue for biodiesel plants and generated a surplus of glycerol. A value-added opportunity is needed in order to compensate for disposal-associated costs. Microbial conversions from glycerol to valuable chemicals performed by various bacteria, yeast, fungi, and microalgae are discussed in this review paper, as well as the possibility of extending these conversions to microbial electrochemical technologies.

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Section: Klebsiella Speciesmentioning

confidence: 99%

Section: Klebsiella Speciesmentioning

confidence: 99%

Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products

Lesnik

Liang

2013

Energies

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“…e To calculate specific productivity, we divided the 1,3-PD production at the end of fermentation by the maximal CDW and by the hours of fermentation. f The data for strain YMU2 are from our previous report (35).…”

Section: Resultsmentioning

confidence: 99%

Novel Redox Potential-Based Screening Strategy for Rapid Isolation of Klebsiella pneumoniae Mutants with Enhanced 1,3-Propanediol-Producing Capability

Zhang

et al. 2007

Appl Environ Microbiol

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This report describes a novel redox potential (oxidoreduction potential [ORP])-based screening strategy for the isolation of mutants of Klebsiella pneumoniae which have an increased ability to produce 1,3-propanediol (1,3-PD). This method can be described as follows: first, to determine an ORP range which is preferred for the wild-type strain to grow and to produce 1,3-PD; second, to subject a chemically mutagenized culture to a reduced ORP level which is deleterious for the wild-type strain. Colonies that showed high specific growth rates at deleterious ORP levels were selected, and their abilities to produce 1,3-PD were investigated. In an ORP-based screening experiment where the ORP was controlled at ؊280 mV, 4 out of 11 isolated strains were recognized as positive mutant strains. A mutant which is capable of producing higher concentrations of 1,3-PD was subjected to fed-batch fermentations for further characterization. Its preferred ORP level (؊280 mV) was significantly lower than that of its parent (؊190 mV). The highest 1,3-PD concentration of the mutant was 915 mmol liter ؊1 , which was 63.1% higher than that of the parent. Metabolic-flux analysis suggested that the intracellular reductive branch of the mutant was strengthened, which improved 1,3-PD biosynthesis. The procedure and results presented here provide a novel method of screening for strains with improved product formation.

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“…Escherichia coli DH5α and its derivatives were routinely grown aerobically in LB medium (37 °C, 180 rpm) (Jian et al 2010;Zhang et al 2006). ISP4-containing 80 mmol l −1…”

Section: Media and Culture Conditionsmentioning

confidence: 99%

Protein expression analysis of a high-demeclocycline producing strain of Streptomyces aureofaciens and the roles of CtcH and CtcJ in demeclocycline biosynthesis

Lin

et al. 2016

Bioresour. Bioprocess.

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Background: Streptomyces aureofaciens strain A6-9, obtained with traditional mutagenesis, produces elevated levels of 6-DCT. The increased formation of 6-DCT may be attributable to the changes in the expression of some proteins in the 6-DCT biosynthetic pathway. For this reason, we explored the differences in protein expression between A6-9 and wild-type (WT) strains of Streptomyces aureofaciens, and based on the differences (CtcH and CtcJ were overexpressed in A6-9), investigated the roles of CtcH and CtcJ in biosynthesis.Results: Two-dimensional gel electrophoresis and a Mascot search indicated that some enzymes (including CtcH and CtcJ) involved in the primary and secondary metabolism were more strongly expressed in the high-6-DCT-yielding strain A6-9 than in the WT strain DT1. To examine the roles of CtcH and CtcJ in 6-DCT biosynthesis, ctcH-deleted, ctcJdeleted, ctcH-overexpressing, and ctcJ-overexpressing mutants and a mutant overexpressing both ctcH and ctcJ were constructed. Compared with WT, 6-DCT production was 50 and 37 % higher in the ctcH-overexpressing and ctcJ-overexpressing strains, respectively, and increased by 60 % in the ctcH-ctcJ-overexpressing strain. The ctcH-deleted and ctcJ-deleted strains produced almost no 6-DCT. Analysis of the metabolic flux distribution indicated that ctcH encodes a hydroxyacyl-CoA dehydrogenase and ctcJ encodes a monooxygenase that are essential for 6-DCT biosynthesis. Conclusion:Protein expression differs between high-6-DCT-yielding and WT strains, and the enzymes increased in the high-6-DCT-yielding strain explain the increased 6-DCT production. ctcH encodes a hydroxyacyl-CoA dehydrogenase and ctcJ encodes a monooxygenase that are essential for 6-DCT biosynthesis.

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Inactivation of aldehyde dehydrogenase: A key factor for engineering 1,3-propanediol production by Klebsiella pneumoniae

Cited by 121 publications

References 21 publications

Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products

Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products

Novel Redox Potential-Based Screening Strategy for Rapid Isolation of Klebsiella pneumoniae Mutants with Enhanced 1,3-Propanediol-Producing Capability

Protein expression analysis of a high-demeclocycline producing strain of Streptomyces aureofaciens and the roles of CtcH and CtcJ in demeclocycline biosynthesis

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