Inactivation of Aspergillus species in real water matrices using medium pressure mercury lamps

Oliveira, B.R.; Marques, Ana Paula; Ressurreição, M.; Moreira, Carlos J. S.; Pereira, Cristina Silva; Crespo, Maria Teresa Barreto; Pereira, Vanessa J.

doi:10.1016/j.jphotobiol.2021.112242

Cited by 8 publications

(5 citation statements)

References 43 publications

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“…It is well known that multiple factors are able to inhibit fungal spores’ germination; in the present study, the disruption of the membrane permeability of the fungal spores was evaluated using confocal laser scanning microscopy coupled with CFDA and PI dyes. This methodology was already reported to be suitable for fungal spores analysis [ 51 ]. Our results showed that the inhibition of A. rugulovalvus spore germination was mainly due to the rupture of the spore permeability barrier after treatment.…”

Section: Resultsmentioning

confidence: 99%

Influence of Atmospheric Cold Plasma Exposure on Naturally Present Fungal Spores and Physicochemical Characteristics of Sundried Tomatoes (Solanum lycopersicum L.)

Molina-Hernández

Laika

Peralta-Ruiz

et al. 2022

Foods

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This research aimed to evaluate the impact of atmospheric cold plasma (ACP) treatment on the fungal spores naturally present in sundried tomatoes, as well as their influence on the physico-chemical properties and antioxidant activity. ACP was performed with a Surface Dielectric Barrier Discharge (SDBD), applying 6 kV at 23 kHz and exposure times up to 30 min. The results showed a significant reduction of mesophilic aerobic bacteria population and of filamentous fungi after the longer ACP exposure. In particular, the effect of the treatment was assessed on Aspergillus rugulovalvus (as sensible strain) and Aspergillus niger (as resistant strain). The germination of the spores was observed to be reliant on the species, with nearly 88% and 32% of non-germinated spores for A. rugulovalvus and A. niger, respectively. Fluorescence probes revealed that ACP affects spore viability promoting strong damage to the wall and cellular membrane. For the first time, the sporicidal effect of ACP against A. rugulovalvus is reported. Physicochemical parameters of sundried tomatoes such as pH and water activity (aw) were not affected by the ACP treatment; on the contrary, the antioxidant activity was not affected while the lycopene content was significantly increased with the increase in ACP exposure time (p ≤ 0.05) probably due to increased extractability.

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Section: Resultsmentioning

confidence: 99%

Influence of Atmospheric Cold Plasma Exposure on Naturally Present Fungal Spores and Physicochemical Characteristics of Sundried Tomatoes (Solanum lycopersicum L.)

Molina-Hernández

Laika

Peralta-Ruiz

et al. 2022

Foods

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“…For example, A. fumigatus can be grown in 5% carbon dioxide, very low pH (3.5), and under ultraviolet radiation. Proteome analyses have shown that stress responses, including cell wall reorganization, DNA repair, and oxidative stress responses during citric acid and itaconic acid production, are increased to overcome radiation effects (Alonso et al 2017;Oliveira et al 2021). However, these data constitute only a small component of A. fumigatus survival mechanisms.…”

Section: Fungal Adaption In the Natural Environmentmentioning

confidence: 99%

Aspergillus fumigatus escape mechanisms from its harsh survival environments

Liu,

Zeng,

Zhou

et al. 2024

Appl Microbiol Biotechnol

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Aspergillus fumigatus is a ubiquitous pathogenic mold and causes several diseases, including mycotoxicosis, allergic reactions, and systemic diseases (invasive aspergillosis), with high mortality rates. In its ecological niche, the fungus has evolved and mastered many reply strategies to resist and survive against negative threats, including harsh environmental stress and deficiency of essential nutrients from natural environments, immunity responses and drug treatments in host, and competition from symbiotic microorganisms. Hence, treating A. fumigatus infection is a growing challenge. In this review, we summarized A. fumigatus reply strategies and escape mechanisms and clarified the main competitive or symbiotic relationships between A. fumigatus, viruses, bacteria, or fungi in host microecology. Additionally, we discussed the contemporary drug repertoire used to treat A. fumigatus and the latest evidence of potential resistance mechanisms. This review provides valuable knowledge which will stimulate further investigations and clinical applications for treating and preventing A. fumigatus infections. Key points • Harsh living environment was a great challenge for A. fumigatus survival. • A. fumigatus has evolved multiple strategies to escape host immune responses. • A. fumigatus withstands antifungal drugs via intrinsic escape mechanisms.

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“…In subsequent studies, Oliveira et al [153,154] establish that exposure to LEDs emitting light at 265 nm generates mitochondrial dysfunctions such as ROS production, excessive melanin deposition in the cell wall, the production of heat shock proteins, and DNA repair proteins. After exposure to UV-LED irradiation, no new proteins were detected in A. niger.…”

Section: Gwmentioning

confidence: 99%

Alternative and Classical Processes for Disinfection of Water Polluted by Fungi: A Systematic Review

Caicedo-Bejarano,

Morante-Caicedo,

Castro-Narváez

et al. 2024

Water

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The introduction of the first list of priority pathogenic fungi by the World Health Organization stresses the need to research and develop public health actions to mitigate infections caused by fungi. One of those actions involves the water disinfection systems, which comprise classical and alternative methods that have been developed in the last decades. Thereby, this work reviews the disinfection of fungi by classical methods such as chlorination, ozonation, and ultraviolet (UV) treatments and alternative advanced oxidation processes (AOPs) such as photo-Fenton, photocatalysis, or couplings of UV with peroxides. The matrices of aquatic systems (sewage, groundwater, drinking water, among others) were considered. A bibliometric analysis is performed initially, and then some aspects of the resistance to antifungals are presented, and the efficiency of the diverse processes in the reduction in fungal loading is also revised. Herein, it is shown the role of the disinfecting agents (e.g., chlorine, hydroxyl radical, or light) and their effects on fungi structures (e.g., direct DNA damage, or indirect damage due to the action of radicals). Moreover, gaps, such as the treatment of antifungal-resistant fungi and limited information about combinations among AOPs, related to the disinfection of water polluted by fungi, were identified.

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Inactivation of Aspergillus species in real water matrices using medium pressure mercury lamps

Cited by 8 publications

References 43 publications

Influence of Atmospheric Cold Plasma Exposure on Naturally Present Fungal Spores and Physicochemical Characteristics of Sundried Tomatoes (Solanum lycopersicum L.)

Influence of Atmospheric Cold Plasma Exposure on Naturally Present Fungal Spores and Physicochemical Characteristics of Sundried Tomatoes (Solanum lycopersicum L.)

Aspergillus fumigatus escape mechanisms from its harsh survival environments

Alternative and Classical Processes for Disinfection of Water Polluted by Fungi: A Systematic Review

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