Inactivation of Dermatophytes Causing Onychomycosis Using Non-Thermal Plasma as a Prerequisite for Therapy

Lokajová, Eliška; Julák, Jaroslav; Khun, Josef; Soušková, Hana; Dobiáš, Radim; Lux, Jaroslav; Scholtz, Vladimír

doi:10.3390/jof7090715

Cited by 7 publications

(2 citation statements)

References 30 publications

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“…The presented work focused on the antivirulence activity of cometary type of discharge for generation of NTP. Our research group developed it couple decades ago and since then its activity towards many pathogenic microorganisms including P. aeruginosa was tested (Scholtz and Julaḱ, 2010a;Scholtz and Julaḱ, 2010b;Scholtz et al, 2010;Julaḱ et al, 2011;Julaḱ et al, 2012;Julaḱ et al, 2013;Scholtz et al, 2013;Scholtz et al, 2015;Khun et al, 2018;Paldrychováet al, 2019;Paldrychováet al, 2020;Vanǩovaé t al., 2020b;Lokajováet al, 2021;Scholtz et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Non-thermal plasma causes Pseudomonas aeruginosa biofilm release to planktonic form and inhibits production of Las-B elastase, protease and pyocyanin

Kašparová

Vaňková²,

Paldrychová³

et al. 2022

Front. Cell. Infect. Microbiol.

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The increasing risk of antibiotic failure in the treatment of Pseudomonas aeruginosa infections is largely related to the production of a wide range of virulence factors. The use of non-thermal plasma (NTP) is a promising alternative to antimicrobial treatment. Nevertheless, there is still a lack of knowledge about the effects of NTP on the virulence factors production. We evaluated the ability of four NTP-affected P. aeruginosa strains to re-form biofilm and produce Las-B elastase, proteases, lipases, haemolysins, gelatinase or pyocyanin. Highly strains-dependent inhibitory activity of NTP against extracellular virulence factors production was observed. Las-B elastase activity was reduced up to 82% after 15-min NTP treatment, protease activity and pyocyanin production by biofilm cells was completely inhibited after 60 min, in contrast to lipases and gelatinase production, which remained unchanged. However, for all strains tested, a notable reduction in biofilm re-development ability was depicted using spinning disc confocal microscopy. In addition, NTP exposure of mature biofilms caused disruption of biofilm cells and their dispersion into the environment, as shown by transmission electron microscopy. This appears to be a key step that could help overcome the high resistance of P. aeruginosa and its eventual elimination, for example in combination with antibiotics still highly effective against planktonic cells.

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Section: Discussionmentioning

confidence: 99%

Non-thermal plasma causes Pseudomonas aeruginosa biofilm release to planktonic form and inhibits production of Las-B elastase, protease and pyocyanin

Kašparová

Vaňková²,

Paldrychová³

et al. 2022

Front. Cell. Infect. Microbiol.

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“…For ease of use, the source of NTP is made in a compact plastic case printed on a 3D printer and is a ready-made device. The NTP source based on the point-to-ring discharge has already been successfully used for the inactivation of fungi [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Non-Thermal Plasma Sources Based on Cometary and Point-to-Ring Discharges

et al. 2021

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A non-thermal plasma (NTP) is a promising tool against the development of bacterial, viral, and fungal diseases. The recently revealed development of microbial resistance to traditional drugs has increased interest in the use of NTPs. We have studied and compared the physical and microbicidal properties of two types of NTP sources based on a cometary discharge in the point-to-point electrode configuration and a corona discharge in the point-to-ring electrode configuration. The electrical and emission properties of both discharges are reported. The microbicidal effect of NTP sources was tested on three strains of the bacterium Staphylococcus aureus (including the methicillin-resistant strain), the bacterium Pseudomonas aeruginosa, the yeast Candida albicans, and the micromycete Trichophyton interdigitale. In general, the cometary discharge is a less stable source of NTP and mostly forms smaller but more rapidly emerging inhibition zones on agar plates. Due to the point-to-ring electrode configuration, the second type of discharge has higher stability and provides larger affected but often not completely inhibited zones. However, after 60 min of exposure, the NTP sources based on the cometary and point-to-ring discharges showed a similar microbicidal effect for bacteria and an individual effect for microscopic fungi.

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Inactivation of Pseudovirus Expressing the D614G Spike Protein Mutation using Nitric Oxide‐Plasma Activated Water

Patel,

Kaushik,

Acharya

et al. 2024

Advanced Science

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Variants of concern (VOCs) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) exhibit high infectivity due to mutations, particularly in the spike protein, that facilitate enhanced binding of virus to human angiotensin‐converting enzyme 2 (hACE2). The D614G mutation, situated in S1‐domain, promotes the open conformation of spike protein, augmenting its interaction with hACE2. Activated water neutralizes pathogens by damaging biological molecules; however, its effect on mutated SARS‐CoV‐2 or VOCs requires further exploration. Here, the efficacy of nitric oxide (NOx)‐plasma activated water (PAW) in inhibiting infections by SARS‐CoV‐2 pseudovirus expressing D614G‐mutated spike protein is investigated, which serves as a model for mutated SARS‐CoV‐2. Results demonstrated high prevalence of D614G mutation in SARS‐CoV‐2 and its VOCs. NOx‐PAW is non‐toxic to cells at high concentration, inhibiting infection by 71%. Moreover, NOx‐PAW induced structural changes in S1‐domain of spike protein, reducing its binding affinity and lowering clathrin‐mediated endocytosis‐related gene expression. Additionally, in silico analysis revealed NOx species in NOx‐PAW played key role in impairing S1‐domain function of the mutated SARS‐CoV‐2 pseudovirus by interacting directly with it. Collectively, these findings reveal the potent inactivation ability of PAW against mutated SARS‐CoV‐2 and suggest its potential application in combating emerging variants of SARS‐CoV‐2 and other viral threats.

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Inactivation of Dermatophytes Causing Onychomycosis Using Non-Thermal Plasma as a Prerequisite for Therapy

Cited by 7 publications

References 30 publications

Non-thermal plasma causes Pseudomonas aeruginosa biofilm release to planktonic form and inhibits production of Las-B elastase, protease and pyocyanin

Non-thermal plasma causes Pseudomonas aeruginosa biofilm release to planktonic form and inhibits production of Las-B elastase, protease and pyocyanin

Non-Thermal Plasma Sources Based on Cometary and Point-to-Ring Discharges

Inactivation of Pseudovirus Expressing the D614G Spike Protein Mutation using Nitric Oxide‐Plasma Activated Water

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