Inactivation of multidrug-resistant pathogens and Yersinia enterocolitica with cold atmospheric-pressure plasma on stainless-steel surfaces

Lis, Karolina Anna; Kehrenberg, Corinna; Boulaaba, Annika; Köckritz-Blickwede, Maren von; Binder, Sylvia; Li, Yangfang; Zimmermann, Julia L.; Pfeifer, Yvonne; Ahlfeld, Birte

doi:10.1016/j.ijantimicag.2018.08.023

Cited by 23 publications

(23 citation statements)

References 28 publications

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“…Here, we found that LPS is degraded by DBD plasma torch treatment. These findings are consistent with a previous study, in which the authors proposed that cell surface components are important in the mechanism of inactivation because the thickness of the polysaccharide membrane influences sensitivity to plasma [ 17 ]. Furthermore, as previously determined [ 28 ], the temperature of the liquid surface was 38.40 ± 0.12 °C at the 2 min treatment time using the same DBD plasma torch under identical conditions.…”

Section: Discussionsupporting

confidence: 93%

“…For example, glow discharge plasma has been shown to inactivate antibiotic resistant E. coli [ 16 ]. Surface micro-discharge (SMD) plasma treatment was demonstrated to inactivate antibiotic-resistant bacteria including Yersinia enterocolitica , Staphylococcus aureus , Klebsiella , E. coli , Acinetobacter , and Enterococcus faecium [ 17 ]. Moreover, a floating-electrode DBD plasma device has successfully inactivated methicillin-resistant S. aureus (MRSA) [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, plasma technologies have been shown to effectively inactivate various bacteria including antibiotic-resistant bacteria [ 16 , 17 ]. Currently, however, it is not known whether the sensitivity against plasma differs between antibiotic-resistant and non-resistant bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

Sakudo

Misawa

2020

IJMS

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Here, we examined whether antibiotic-resistant and non-resistant bacteria show a differential susceptibility to plasma treatment. Escherichia coli DH5α were transformed with pPRO-EX-HT-CAT, which encodes an ampicillin resistance gene and chloramphenicol acetyltransferase (CAT) gene, and then treated with a dielectric barrier discharge (DBD) plasma torch. Plasma treatment reduced the viable cell count of E. coli after transformation/selection and further cultured in ampicillin-containing and ampicillin-free medium. However, there was no significant difference in viable cell count between the transformed and untransformed E. coli after 1 min- and 2 min-plasma treatment. Furthermore, the enzyme-linked immunosorbent assay (ELISA) and acetyltransferase activity assay showed that the CAT activity was reduced after plasma treatment in both transformed and selected E. coli grown in ampicillin-containing or ampicillin-free medium. Loss of lipopolysaccharide and DNA damage caused by plasma treatment were confirmed by a Limulus test and polymerase chain reaction, respectively. Taken together, these findings suggest the plasma acts to degrade components of the bacteria and is therefore unlikely to display a differential affect against antibiotic-resistant and non-resistant bacteria. Therefore, the plasma method may be useful in eliminating bacteria that are recalcitrant to conventional antibiotic therapy.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

Sakudo

Misawa

2020

IJMS

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“…Previously, numerous studies showed that various CAP treatment methods (including CAP‐treated liquids) are capable of eradicating microorganisms including multidrug‐resistant strains. [ 25,34 ] Furthermore, in a previous study, we have shown the broad‐spectrum antimicrobial activity of CAP‐treated DIW, PBS, and NAC. [ 25 ] Therefore, the delivery of CAP‐treated liquids by nebulization seems to be a feasible method for control and prevention of VAP, as they do not cause obstruction of airways.…”

Section: Discussionmentioning

confidence: 94%

“…The strong and broad‐spectrum antimicrobial activity of CAP is mainly attributed to generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) during plasma formation. [ 34,36 ] While CAP is being formed, numerous ROS, including hydrogen peroxide, superoxide, singlet oxygen, atomic oxygen, ozone, and hydroxyl radicals, and RNS, including nitric oxide, nitrous acid, peroxynitrite, and peroxynitrous, are formed. Thus, CAP is a complex mixture of ROS and RNS, and their combination leads to microbial inactivation.…”

Section: Discussionmentioning

confidence: 99%

Inactivation of biofilms in endotracheal tube by cold atmospheric plasma treatment for control and prevention of ventilator‐associated pneumonia

Ibis

Ercan

2020

Plasma Processes & Polymers

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Ventilator-associated pneumonia (VAP) is a type of hospital-acquired infection with high mortality and morbidity rates that is seen in critically ill patients who are connected to mechanical ventilators in intensive care units. Conventional infection control measures may remain insufficient for control and prevention of VAP. In the present study, biofilms of most common pathogens, which are responsible for VAP, namely Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, and Candida albicans, were grown in the lumen of endotracheal tubes (ETs) under low shear to mimic the biofilm formation in clinical settings using the drip flow reactor system. Antibiofilm efficacies of two different plasma treatment methods were tested on the biofilms that were grown in ETs. In the first method, deionized water (DIW), phosphate-buffered saline (PBS) solution, and N-acetylcysteine (NAC) solution were treated with air dielectric barrier discharge (DBD) plasma and then nebulized on the biofilms. In the second method, air plasma afterglow (APA), which was generated using a DBD jet electrode, was flown through the lumen of ETs. Effects of two different plasma treatment methods on biofilms were evaluated using colony-counting and XTT assays. The quantity of extracellular polymeric substance (EPS) was evaluated with the safranin assay. Scanning electron microscopy (SEM) was used to visualize the effect of various plasma treatment methods on biofilms. Cytotoxic effects of two different plasma treatment methods were tested on the human tracheal epithelial cell line. Our results suggest that the nebulized plasma-treated NAC solution had a superior antibiofilm effect as compared with DIW and PBS. Two different plasma treatment methods used effectively inactivated biofilms and significantly reduced the quantity of EPS without a significant damage to the human tracheal epithelial cell line. Furthermore, SEM images showed the inactivation of microbial cells mainly through membrane damage. K E Y W O R D S cold plasma, dielectric barrier discharges, endotracheal tube, plasma medicine, ventilatorassociated pneumonia Fatma İbiş and Utku K. Ercan contributed equally to this study.

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Cold Plasma Therapy as a Physical Antibiofilm Approach

Abdo

Schmitt-John

Richter

2022

Springer Series on Biofilms

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Inactivation of multidrug-resistant pathogens and Yersinia enterocolitica with cold atmospheric-pressure plasma on stainless-steel surfaces

Cited by 23 publications

References 28 publications

Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

Inactivation of biofilms in endotracheal tube by cold atmospheric plasma treatment for control and prevention of ventilator‐associated pneumonia

Cold Plasma Therapy as a Physical Antibiofilm Approach

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