Inactivation of mycoplasma in serum with binary ethyleneimine

Abstract

A method of inactivating mycoplasma contaminants in sera by using binary ethyleneimine was tried. When used at the concentration recommended for inactivating viruses, 0.001 M, binary ethyleneimine inactivated only two of five mycoplasma species. Even at a concentration of 0.01 M, the compound did not consistently inactivate all mycoplasmas.

Quantitative detection and inactivation of Mycoplasma hyopneumoniae.

“…hyopneumoniae was completely inactivated by treatment with 0.01% formaldehyde for 24 h or 0.02% formaldehyde for 12 h at 37℃ and treatment with 0.004% BEI for 24 h or 0.5% BEI for 12 h at 37℃. These findings are consistent with the results that M. hyorhinis was completely inactivated by 0.001M (0.0067%) BEI (Christianson et al, 1980). A 2010 study found that 22 mycoplasma species examined were completely inactivated within 3-24 h at room temperature in mycoplasma media containing 0.2% (66.60 mM) formaldehyde and 0.1% (13.87 mM) BPL .…”

“…All treated cultures were incubated for 12 h and 24 h and thereafter assay for residual live mycopslama (see section 3.2.3). And the end of the inactivation, samples inactivated with BEI were hydrolyzed by adding sterile sodium thiosulfate solution to a final concentration of 2% (Christianson et al, 1980). Inactivated samples were stored at 4℃ until further use.…”