Inactivation of plant-pathogenic fungus Colletotrichum acutatum with natural plant-produced photosensitizers under solar radiation

Fracarolli, Letícia; Rodrigues, Gabriela Braga; Pereira, Ana Carolina; Júnior, Nelson Sidnei Massola; Silva, Geraldo José da; Bachmann, Luciano; Wainwright, Mark; Bastos, Jairo Kenupp; Braga, Gilberto Úbida Leite

doi:10.1016/j.jphotobiol.2016.07.009

Cited by 38 publications

(34 citation statements)

References 55 publications

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“…We supposed that in the field such concentration will have no effect on plants treated by spraying. Nevertheless, studies on plant pathogens as fungi showed that a concentration up to 50 μ M of natural and synthetic photosensitizers was required to eradicate fungi (De Menezes et al , , Fracarolli et al ). At this threshold concentration of cationic porphyrins, Arabidopsis and pathogens will be most likely eradicated whereas this concentration does not kill tomato plantlets (Guillaumot et al ).…”

Section: Discussionmentioning

confidence: 99%

“…It could be envisaged as a farming practice in order to eradicate plant pathogens as bacteria and fungi that cause serious threats by drastically reducing crops yield. Studies focusing on the side effects of APDT directly on plants are rare (De Menezes et al , , Fracarolli et al , De Menezes et al , Guillaumot et al , Gonzales et al 2017). However, such studies are required to evaluate the efficiency of APDT.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, it was reported that natural photosensitizers such as coumarins and furocoumarins or synthetic ones such as phenothiazinium inactivated pathogenic fungi ( Colletotrichum , Aspergillus and Fusarium ). When spotted on orange tree or strawberry plants, orange tree leaves were not affected by either natural/synthetic photosensitizers whereas they damaged strawberry leaves when tested at 100 μ M (De Menezes et al , , Fracarolli et al ). Our aim is to explore, the phenotypic and molecular responses of plantlets growing in vitro under photodynamic stress induced by photoactivated porphyrins included in growth medium.…”

Section: Introductionmentioning

confidence: 99%

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Responses of an adventitious fast‐growing plant to photodynamic stress: comparative study of anionic and cationic porphyrin effect on Arabidopsis thaliana

Issawi

Guillaumot

Sol

et al. 2017

Physiologia Plantarum

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Antimicrobial photodynamic treatment (APDT) based on the use of a photosensitizer to produce reactive oxygen species (ROS) that induce cell death could be envisaged to fight against plant pathogens. For setting this strategy, we want to study how plants themselves respond to photodynamic treatment. In previous work we showed that tomato plantlets were able to resist photoactivated tetra (N-methylpyridyl) porphyrin (CP) or the zinc metalated form (CP-Zn). To enlarge our plant expertise related to exogenous porphyrins treatment and to further defend this approach, we studied how a weed like Arabidopsis thaliana responded to exogenous supply of anionic and cationic porphyrins. Both types of photosensitizers had no negative effect on seed germination and did not hamper the development etiolated Arabidopsis plantlet under dark conditions. Thus, post-emergence effects of porphyrin photoactivation on the development of 14 day-old in vitro Arabidopsis plantlet under light were observed. CP-Zn was the most efficient photosensitizer to kill Arabidopsis plantlets while anionic tetra (4-sulfonatophenyl) porphyrin only delayed their growth and development. Indeed only 7% of plantlets could be rescued after CP-Zn treatment. Furthermore, non-enzymatic and enzymatic defense components involved in detoxification of ROS generated by CP-Zn under illumination were downregulated or stable with the exception of sevenfold increase in proline content. As previously demonstrated in the literature for microbial agents and in the present work for Arabidopsis, CP-Zn was efficient enough to eradicate unwanted vegetation and plant pathogens without at the same time killing plants of agronomic interest such as tomato plantlets.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Responses of an adventitious fast‐growing plant to photodynamic stress: comparative study of anionic and cationic porphyrin effect on Arabidopsis thaliana

Issawi

Guillaumot

Sol

et al. 2017

Physiologia Plantarum

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“…Porphyrins and chlorins such as chlorophyllin, have been shown to be very effective against bacteria on kiwi leaves tested in vitro (Jesus et al 2018;Glueck et al 2019). Coumarins, furocoumarin and phenothiazinium were shown to be active against the plant-infecting fungi Colletrichum acuratum and Aspergillus nidulans (De Menezes et al a, b;2014;Fracarolli et al 2016). Finally, when tested on Citrus sinensis petals and leaves, methylene blue was able to kill the Colletotrichum abscissum fungus and proved to be harmless to plant organs; in addition, this treatment did not induce any secondary resistance (Gonzales et al 2017) Previous research works explored the in vitro phenotypical and molecular responses of Arabidopsis and tomato plantlets to the photodynamic stress induced by an exogenous supply of PS (Guillaumot et al 2016;Issawi et al 2018b).…”

Section: Introductionmentioning

confidence: 99%

Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

Ambrosini¹,

Issawi²,

Sol³

et al. 2020

Preprint

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Botrytis cinerea is a necrotic fungal plant pathogen responsible for the gray mold disease on more than 200 crops, including grapevine. Due to its genetic plasticity, this fungus presents a strong resistance to a large spectrum of fungicides. Thus, new fighting strategies against B. cinerea are urgently needed. In this context, antimicrobial photodynamic treatment (APDT) was considered. APDT involves the use of a photosensitizer that generates reactive oxygen species upon illumination. Tetra-4-sulfonatophenyl porphyrin tetra-ammonium (TPPS) was tested on B. cinerea; upon light exposure. 1.5 µM TPPS was shown to completely inhibit mycelial growth. A concentration of 12.5 µM TPPS was tested on three genetic background clones from Chardonnay, Merlot and Sauvignon, grown in vitro for 2 months. Treated root apparatus of the three backgrounds was able to increase thiol production as a molecular protection against photoactivated TPPS, leading to a normal phenotype as compared with control plantlets. Finally, two-month-old grapevine leaves were infected with four-day-old mycelium of B. cinerea pre-incubated or not with TPPS. The pre-treated mycelium was unable to infect the isolated leaves of any of the three grapevine varieties after 72 h growth when subjected to a 16 h photoperiod, contrary to untreated mycelium. These results on fungus, plantlets and Botrytis-grapevine leaves, in contact with a very low concentration of TPPS, suggest a strong potential of photo-treatment against Botrytis mycelium for future agricultural practices in vineyard or other cultures.

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“…Furocoumarins (FCs), also named psoralens, are naturally occurring metabolites in the plants belonging to the Rutaceae and Umbelliferae families, in which they are produced as a chemical defence in response to stress, fungi, bacteria, and insects . With their ambiguous effects on human health, FCs have been widely investigated and have attracted great interest in comparison with the other two classes of molecules reported here.…”

Section: Introductionmentioning

confidence: 99%

Oxygen heterocyclic compound screening in Citrus essential oils by linear retention index approach applied to liquid chromatography coupled to photodiode array detector

Arigò

Rigano

Micalizzi

et al. 2019

Flavour & Fragrance J

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To date, liquid chromatography coupled to a photodiode array (PDA) detector is the main technique employed to analyse furocoumarins (FCs) in essential oils. This type of detection may lead to poor performance because of the low sensitivity and selectivity of PDA detection. In this work, the linear retention index (LRI) was used in combination with a UV‐Vis library for the identification of 35 oxygen heterocyclic compounds in Citrus essential oils, making it possible to distinguish compounds with very similar spectra. As opposed to retention time, which can significantly change with the instrument or laboratory used, LRI represents a kind of normalized relative retention time, as independent as possible from operating conditions. From a qualitative point of view, reproducibility results are provided to definitively assess the suitability of such an approach for inter‐laboratory use and for a fully automated process, even when pure and very expensive standard materials are not available, as just the LRI database and the UV‐Vis library are needed. From a quantitative point of view, calibration curves were created in pure solvent and by adding standard compounds to several blank matrices, with the aim of evaluating differences, overcoming matrix effects, achieving the correct limits of quantification, and providing the quantitative profile of real samples. The LRI system is not listed in the Commission Decision 2002/657/EC as criteria for the Full Scan UV‐Vis detection; however, its validity and applicability are here investigated and proposed for future applications, in combination with the UV‐Vis library.

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Inactivation of plant-pathogenic fungus Colletotrichum acutatum with natural plant-produced photosensitizers under solar radiation

Abstract: 23The increasing tolerance to currently used fungicides and the need for environmentally

Cited by 38 publications

References 55 publications

Responses of an adventitious fast‐growing plant to photodynamic stress: comparative study of anionic and cationic porphyrin effect on Arabidopsis thaliana

Responses of an adventitious fast‐growing plant to photodynamic stress: comparative study of anionic and cationic porphyrin effect on Arabidopsis thaliana

Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

Oxygen heterocyclic compound screening in Citrus essential oils by linear retention index approach applied to liquid chromatography coupled to photodiode array detector

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