Inactivation of Poliomyelitis Virus in Vitro by Crystalline Vitamin C (Ascorbic Acid)

Jungeblut, Claus W.

doi:10.1084/jem.62.4.517

Cited by 37 publications

(12 citation statements)

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“…Ascorbate is one of the early unorthodox therapies for infectious disease and cancer since its introduction in the first half of the 20th century [2][3][4][7][8][9]60,61]. This approach was subsequently promoted by Cameron and Pauling [5,6,62,63].…”

Section: Discussionmentioning

confidence: 99%

Pharmacological ascorbate induces cytotoxicity in prostate cancer cells through ATP depletion and induction of autophagy

Chen

Chalmers

et al. 2012

Anti-Cancer Drugs

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Recent studies have revealed the scientific basis for the use of intravenous (i.v.) vitamin C or ascorbic acid (ascorbate) in treating cancers, and raised the possibility of using i.v. ascorbate as a prooxidant anticancer therapy. Through the production of H2O2, pharmacologic ascorbate can induce some cancer cell death in vitro and inhibit a number of types of tumor growth in animal models. However, the mechanism of cell death triggered by ascorbate is not well understood. In this study, we investigated the cytotoxicity of pharmacological concentrations of ascorbate to human prostate cancer cells and the mechanisms involved. The results showed that ascorbate in the millimolar range induced cytotoxicity in five of the six tested prostate cancer cell lines. The IC50 values in the sensitive prostate cancer cells ranged from 1.9 to 3.5 mmol/l, concentrations clinically achievable with i.v. ascorbate use. All tested androgen-independent cells were sensitive to ascorbate treatment. The ascorbate-insensitive cell line LaPC4 is hormonally dependent. Whereas the reasons for sensitivity/resistance to ascorbate treatment need to be investigated further, cell death in sensitive cells was dependent on H2O2. Ascorbate treatment depleted ATP and induced autophagy in sensitive prostate cancer cells, resulting in cell death. Taken together with previous studies, high-dose ascorbate has the potential to be a novel treatment option to hormone-refractory prostate cancer.

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Section: Discussionmentioning

confidence: 99%

Pharmacological ascorbate induces cytotoxicity in prostate cancer cells through ATP depletion and induction of autophagy

Chen

Chalmers

et al. 2012

Anti-Cancer Drugs

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“…The antiviral properties of Vit-C help to reduce symptoms and mortality in children and adults [1][2][3][4]. The antiviral activities of ascorbic acid was known and it was published almost 80 years ago [5][6][7][8][9] when scientists were involved in work on poliomyelitis. Moreover, the use of ascorbic acid as a medicinally crucial agent against various diseases was also well established [10][11][12][13][14][15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Intravenous vitamin C for reduction of cytokines storm in acute respiratory distress syndrome

2020

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“…The controversy surrounding the claim of the antitumor effect of vitamin C has attracted a lot of public attentions (1)(2)(3). Despite reports on the antiviral (4)(5)(6) and antitumor (7)(8)(9) activities of ascorbic acid there is as yet no persuasive explanations of these biological effects at the cellular and molecular levels. Previous studies (9, 10) indicated a preferential cytotoxicity of ascorbate and copper ion against malignant melanoma cells with little effect on the proliferation of normal cells.…”

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confidence: 99%

Specific Cleavages of DNA by Ascorbate in the Presence of Copper Ion or Copper Chelates

Chiou

Chang

Jou

et al. 1985

The Journal of Biochemistry

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The DNA-cleavage specificity of ascorbate in the presence of copper ion is analyzed with end-labeled pBR322 DNA fragments. The nonenzymatic reaction of Cu(II)/ascorbate and DNA shows certain degrees of cleavage preference toward purine-containing short segments in the labeled DNA under mild conditions (at 0 degrees C and 10 min). The segments of pyrimidine clusters are least susceptible to cleavage. The DNA scission cannot be detected in the absence of metal ions, and is greatly diminished in the presence of EDTA and metal-chelating peptide. It is more specific than the nuclease-like scission activity induced by cuprous-phenanthroline complex. This scission activity in relation to the antiviral and antitumor activities of vitamin C reported in the literature deserves a crucial consideration.

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Inactivation of Poliomyelitis Virus in Vitro by Crystalline Vitamin C (Ascorbic Acid)

Cited by 37 publications

References 0 publications

Pharmacological ascorbate induces cytotoxicity in prostate cancer cells through ATP depletion and induction of autophagy

Pharmacological ascorbate induces cytotoxicity in prostate cancer cells through ATP depletion and induction of autophagy

Intravenous vitamin C for reduction of cytokines storm in acute respiratory distress syndrome

Specific Cleavages of DNA by Ascorbate in the Presence of Copper Ion or Copper Chelates

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