Inactivation of Polymorphonuclear-Neutrophil Derived Eosinophil Chemotactic Factor by Human Serum

König, Wolfgang; Czarnetzki, B.M.

doi:10.1159/000232131

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…These studies show that serum proteins are not only necessary for leukocyte chemotaxis but that they have a marked, concen tration-dependent modulating effect on the spontaneous and the directed migration of the cells, as was already demonstrated by our previous studies with crude and frac tionated serum [4,6].…”

Section: Discussionsupporting

confidence: 78%

“…Thus, several serum in hibitors have been reported to occur in hu man disease [1][2][3], and the effect of normal serum components on chemotactic factors and on the migrating cells is not well under stood. So far, several powerful factors of varying molecular weight have been de scribed in normal serum which inactivate the neutrophil-derived eosinophil chemotactic factor (ECF) [4], The immunoglobu lins IgM and IgA inhibit polymorphonu clear leukocyte chemotaxis by cell-directed mechanisms [4,5], and migrating cells are also the targets of two high-molecular weight proteins which enhance rather than inhibit eosinophil chemotaxis [6],…”

Section: Introductionmentioning

confidence: 99%

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Role of Purified Serum Components in Polymorphonuclear Leukocyte Chemotaxis

Czarnetzki

Schulz²

1980

Int Arch Allergy Immunol

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Three highly purified serum proteins are examined for their role during leukocyte chemotaxis. In the absence of added proteins, migration of eosinophils towards eosinophil chemotactic factor and of neutrophils towards bacterial factor does not occur at all. Dose-response curves with human serum albumin (HSA) show that chemotaxis proceeds well after addition of HSA, with an optimum at 1 mg/ml. HSA has to be present in the buffer during chemotaxis since preincubation of cells followed by washing is ineffective. In contrast,α₂-macroglobulin and α₁-antitrypsin enhance leukocyte migration at doses close to physiological serum levels and affect cells irreversibly. The data suggest a complex and powerful modulating influence of serum proteins on the influx of eosinophils and neutrophils to tissue sites.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Role of Purified Serum Components in Polymorphonuclear Leukocyte Chemotaxis

Czarnetzki

Schulz²

1980

Int Arch Allergy Immunol

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“…In table V this enhanc ing activity is compared to the inactivating activity towards E C F in human serum which was reported previously [6],…”

Section: Discussionmentioning

confidence: 59%

“…In fact, human serum has been shown to con tain several factors that specifically inhibit eosinophil migration either by a direct effect on the cells [5,6] or by destroying the chemotactic factors. Thus, C5a is enzymati cally inactivated by a carboxypeptidase [7] and E C F by SDI [6], a heterogeneous group of inhibitors in normal human serum.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Eosinophil Leukocyte Chemotaxis by a Factor in Normal Human Serum (SEF)

Czarnetzki¹

1979

Int Arch Allergy Immunol

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An activity in normal human serum is described which enhances the migration of eosinophils towards neutrophil-derived eosinophil chemotactic factor (ECF). The serum-enhancing factor (SEF), in contrast to the inhibitors in serum, is expressed primarily during weak chemotaxis, acts in a cell-directed fashion, increases the chemokinesis of eosinophils and, in the presence of ECF, enhances chemotaxis in a synergistic fashion. SEF is found in sera of several mammalian species, affects human and guinea pig eosinophils, and it is heat-labile (56°C). On Sephadex column chromatography, SEF has a molecular weight of ∼800,000 daltons. In some sera, a second activity with a molecular weight of ∼200,000 daltons is eluted. SEF does not enhance eosinophil migration once the cells have been deactivated in vitro by their chemotactic factor. Because of its wide distribution, SEF may play an important modulating role both in in vitro assay systems and in vivo.

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Effect of Neutrophil-derived Eosinophil Chemotactic Factor (ECF) in Human and Guinea Pig Skin

Czarnetzki¹,

Frosch²,

Vakilzadeh³

et al. 1980

Journal of Investigative Dermatology

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The in vivo effectiveness of eosinophil chemotactic factor (ECF), secreted in vitro by human neutrophils (PMN) during phagocytosis, is tested in 2 model systems. Injection of ECF into guinea pig ears causes a preferential attraction of eosinophils with time that is more marked in animals with preexisting eosinophilia. In the same model system, activated serum attracts fewer eosinophils and more PMN. In human skin windows, ECF and activated serum are equally effective on a quantitative basis, but ECF is more selective for eosinophils in patients with eosinophilia. With normal controls, ECF and activated serum attract PMN equally well. The studies confirm previous in vitro observations on the properties of ECF and suggest a potentially significant role of this factor during pathological processes.

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Inactivation of Polymorphonuclear-Neutrophil Derived Eosinophil Chemotactic Factor by Human Serum

Cited by 5 publications

References 15 publications

Role of Purified Serum Components in Polymorphonuclear Leukocyte Chemotaxis

Role of Purified Serum Components in Polymorphonuclear Leukocyte Chemotaxis

Enhancement of Eosinophil Leukocyte Chemotaxis by a Factor in Normal Human Serum (SEF)

Effect of Neutrophil-derived Eosinophil Chemotactic Factor (ECF) in Human and Guinea Pig Skin

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