Inactivation of Schistosoma Using Low-Temperature Plasma

Hejzlarová, Silvie; Chanová, Marta; Khun, Josef; Julák, Jaroslav; Scholtz, Vladimír

doi:10.3390/microorganisms9010032

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…For the past decade, atmospheric pressure and nonthermal plasmas have proven their efficiency as an inactivation tool against bacteria, fungi, and viruses. − A few examples of the uses of the plasma technology include bacterial inactivation, blood coagulation, tumor treatment, and wound healing. − Microbial mutagenesis based on a plasma-microbial interaction has not been considered much in the past. However, the impact of the plasma-based microbial mutagenesis bears huge potential because it can be applied to the mutagenesis of microalgae, bacteria, and yeast. − As plasma generates reactive radical species, such as N 2 *, N 2 + , NO, O, OH, and H α , under noble gases, the radical-treated cells start the SOS repair mechanism, which results in various mismatches in DNA sequence during the repair process. , Compared to traditional mutation methods employing chemical and physical reagents, this in situ plasma treatment has several advantages in cost, work safety, and faster mutation procedure .…”

Section: Introductionmentioning

confidence: 99%

Construction of a Compact Array of Microplasma Jet Devices and Its Application for Random Mutagenesis of Rhodosporidium toruloides

Koh,

Kim,

Rao

et al. 2023

ACS Synth. Biol.

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A small and efficient DNA mutation-inducing machine was constructed with an array of microplasma jet devices (7 × 1) that can be operated at atmospheric pressure for microbial mutagenesis. Using this machine, we report disruption of a plasmid DNA and generation of mutants of an oleaginous yeast Rhodosporidium toruloides. Specifically, a compact-sized microplasma channel (25 × 20 × 2 mm3) capable of generating an electron density of greater than 1013 cm–3 was constructed to produce reactive species (N2*, N2 +, O, OH, and Hα) under helium atmospheric conditions to induce DNA mutagenesis. The length of microplasma channels in the device played a critical role in augmenting both the volume of plasma and the concentration of reactive species. First, we confirmed that microplasma treatment can linearize a plasmid by creating nicks in vitro. Second, we treated R. toruloides cells with a jet device containing 7 microchannels for 5 min; 94.8% of the treated cells were killed, and 0.44% of surviving cells showed different colony colors as compared to their parental colony. Microplasma-based DNA mutation is energy-efficient and can be a safe alternative for inducing mutations compared to conventional methods using toxic mutagens. This compact and scalable device is amenable for industrial strain improvement involving large-scale mutagenesis.

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Section: Introductionmentioning

confidence: 99%

Construction of a Compact Array of Microplasma Jet Devices and Its Application for Random Mutagenesis of Rhodosporidium toruloides

Koh,

Kim,

Rao

et al. 2023

ACS Synth. Biol.

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“…Wang et al [ 48 ] described the inactivation of Schistosoma japonicum cercariae using the dielectric barrier discharge in the stream of carrier He, O 2 or air. Hejzlarová et al [ 49 ] inactivated S. mansoni cercariae and miracidia using NTP produced by three types of DC corona discharges.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Acanthamoeba Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma

et al. 2021

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Water suspensions of cysts of a pathogenic clinical isolate of Acanthamoeba sp. were prepared, and the cysts were inactivated either in suspension or placed on the surface of contact lenses by the non-thermal plasma produced by the DC corona transient spark discharge. The efficacy of this treatment was determined by cultivation and the presence of vegetative trophozoites indicating non-inactivated cysts. The negative discharge appeared to be more effective than the positive one. The complete inactivation occurred in water suspension after 40 min and on contaminated lenses after 50 min of plasma exposure. The properties of lenses seem to not be affected by plasma exposure; that is, their optical power, diameter, curvature, water content and infrared and Raman spectra remain unchanged.

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Atmospheric air and liquid-film DBD plasma using sine AC excitations for purpose of improving the hydrophilicity of PTFE

Wang

Liang

et al. 2023

Vacuum

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Inactivation of Schistosoma Using Low-Temperature Plasma

Cited by 3 publications

References 43 publications

Construction of a Compact Array of Microplasma Jet Devices and Its Application for Random Mutagenesis of Rhodosporidium toruloides

Construction of a Compact Array of Microplasma Jet Devices and Its Application for Random Mutagenesis of Rhodosporidium toruloides

Inactivation of Acanthamoeba Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma

Atmospheric air and liquid-film DBD plasma using sine AC excitations for purpose of improving the hydrophilicity of PTFE

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