Inactivation of <scp><i>Pseudomonas aeruginosa</i></scp> in mineral water by DP1 bacteriophage immobilized on ethylene‐vinyl acetate copolymer used as seal caps of plastic bottles

Novello, Júnia Capua de Lima; Sillankorva, Sanna; Pires, Priscila; Azeredo, Joana; Wanke, Cesar Henrique; Tondo, Eduardo César; Bianchi, Otávio

doi:10.1002/app.49009

Cited by 6 publications

(13 citation statements)

References 35 publications

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“…Numerous efforts have been made to immobilize active compounds onto the polymeric surface, by surface modification or chemical retention [6]. In this last case, both ionic and covalent immobilization require the presence of functional groups on the antimicrobial molecule and on the polymer; very often these systems need further chemical modifications to allow the active molecule being in contact with the target microorganism [7]. The binding of an agent to the surface of the package would require a molecular structure large enough to retain activity on the microbial cell wall even though bound to the plastic.…”

Section: Introductionmentioning

confidence: 99%

Study on the Influence of Bio-Based Packaging System on Sodium Benzoate Release Kinetics

Conte

Lecce

Iannetti

et al. 2020

Foods

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The influence of film structure on the release kinetics of sodium benzoate (SB) from polymeric films is addressed in this study. In particular, four film structures were investigated, two monolayer and two multilayer systems. In particular, in one case, the active substance was uniformly distributed into a chitosan-based matrix, and in the other one, it was previously incorporated into alginate beads before dispersion in the chitosan film, thus realizing two types of monolayer films; on the other hand, the same chitosan film with SB encapsulated in alginate beads was used as the inner layer of a multilayer system constituted by two side films of alginate. The two alginate-based layers were made with two different thicknesses, thus producing a total of two multilayer systems. The release of SB from the above-mentioned films in water was studied by means of a UV/VIS spectrophotometer at 227 nm. A first-order kinetics-type equation was used to quantitatively describe the release data. Results suggest that the film structure strongly affected the release kinetics. In fact, monolayer films showed single-stage release kinetics, whereas the two investigated multilayer systems showed two-stage release kinetics. Further, the presence of alginate beads strongly affected the SB release, thus suggesting the potential of encapsulation to control the release mechanism of active compounds.

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Section: Introductionmentioning

confidence: 99%

Study on the Influence of Bio-Based Packaging System on Sodium Benzoate Release Kinetics

Conte

Lecce

Iannetti

et al. 2020

Foods

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“…Aunque este producto asocia a su imagen una condición de pureza y seguridad sanitaria, la aparición de brotes gastrointestinales debido al consumo de estas aguas ha dirigido la atención de los investigadores a su estudio, especialmente en lo que respecta a su estándar microbiológico (COSTA et al, 2015;WIELAND et al, 2018). Novello et al, (2020) afirman que el crecimiento microbiano rápido después del empaque puede estar relacionado con la oxigenación del agua, el aumento de temperatura durante el almacenamiento y los nutrientes presentes en la superficie del empaque. Se ha observado que este producto no es necesariamente seguro y puede representar riesgos para la salud de los consumidores, como el agravamiento por patógenos oportunistas como Pseudomonas aeruginosa.…”

Section: Introductionunclassified

“…Se ha observado que este producto no es necesariamente seguro y puede representar riesgos para la salud de los consumidores, como el agravamiento por patógenos oportunistas como Pseudomonas aeruginosa. Esta bacteria, debido a su alta resistencia a la terapia antimicrobiana, está asociada con infecciones crónicas persistentes, afectando principalmente a individuos inmunocomprometidos (REIS et al, 2014;NOVELLO et al, 2020).…”

Section: Introductionunclassified

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Formadores de biopelículas de Pseudomonas aeruginosa en agua potable

Cavalcante,

Andrade,

Silva

et al. 2023

Revista Científica Multidisciplinar Núcleo do Conhecimento

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En la actualidad, ha habido un aumento en la demanda de agua embotellada debido a la insatisfacción de la población con la calidad del agua proporcionada por las agencias públicas. El presente estudio buscó evaluar la presencia de P. aeruginosa formadora de biopelículas en agua potable embotellada. Se seleccionaron treinta y cinco muestras típicas de agua embotellada para identificar la presencia de P. aeruginosa y su capacidad para formar biopelículas bacterianas. La metodología utilizada para verificar la presencia de P. aeruginosa siguió la técnica de tubos múltiples, y la evaluación de la capacidad de formación de biopelículas siguió la técnica de microdilución, con la lectura óptica realizada por espectrofotometría a 620 nm. Se detectó la presencia de P. aeruginosa en el 26% de las muestras analizadas, lo que representa nueve de las 35 muestras probadas. De estas, el 55% tenía la capacidad de formar biopelículas. El presente estudio muestra que la presencia de P. aeruginosa, capaz de formar biopelículas, es un factor de riesgo para la salud pública debido a la amplia comercialización de agua embotellada.

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“…even in monsoon and postmonsoon season. In a recent study byNovello et al (2020) bacteriophage have been used in seal caps of the bottled water to preserve the self-life against P. aeruginosa.…”

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confidence: 99%

Microbiological quality assessment (including antibiogram and threat assessment) of bottled water

Gautam

2021

Food Science & Nutrition

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Water pollution is a major global problem that has been the leading cause of morbidity and mortality. This study was carried out to assess the microbiological quality of popular domestic brands of bottled water available in the Kathmandu Valley in Nepal. For the study of bottled water, a total of 50 samples each of different volumes (20 and 1 L) were selected. The samples were processed at the Microbiology laboratory at St. Xavier's College, Maitighar, Kathmandu. The microbiological assessments were performed as per the methods described in the American Public Health Association, 2005. Out of 100 samples, 48% of samples were found to be contaminated with total coliform. Escherichia coli was the predominant strain among the coliforms. Multidrug‐resistant E. coli, Enterobacter aerogenes, and Pseudomonas aeruginosa were isolated from the 48 bottled water. Treat assessment test revealed that 88.23% of the isolated E. coli produced β hemolytic colonies, while 11.77% did not show hemolytic colonies, 100% E. aerogenes colonies were not able to develop hemolytic colonies and 100%, P. aeruginosa colonies gave β hemolytic colonies, respectively. Chi–square test shows that there is a significant association (p ≤ .05) between fecal coliform and volume of the vessel (i.e., 20 and 1 L), bottle type (i.e., unscratched and undented and scratched and/or dented), season (i.e., monsoon and postmonsoon), and total coliform. Chi‐square test shows that there is no significant (p > .05) association between Pseudomonas spp and season (i.e., monsoon and postmonsoon). Out of 100 samples, 48% of samples were found to be contaminated with total coliform. Microorganisms survive in bottled water as they have many nutrients required for the microorganism in ionic form. Surveillance is lacking by the license‐providing organizations followed by governmental organizations.

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Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene‐vinyl acetate copolymer used as seal caps of plastic bottles

Cited by 6 publications

References 35 publications

Study on the Influence of Bio-Based Packaging System on Sodium Benzoate Release Kinetics

Study on the Influence of Bio-Based Packaging System on Sodium Benzoate Release Kinetics

Formadores de biopelículas de Pseudomonas aeruginosa en agua potable

Microbiological quality assessment (including antibiogram and threat assessment) of bottled water

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