Inactivation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by gaseous ozone treatment

Yano, Hisakazu; Nakano, Ryuichi; Suzuki, Yuki; Nakano, Akiyo; Kasahara, Kei; Hosoi, Hiroshi

doi:10.1016/j.jhin.2020.10.004

Cited by 53 publications

(53 citation statements)

References 7 publications

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“…Therefore, it is assumed, that ozone is also effective against SARS-CoV-2. This assumption is also supported by current literature reviews and initial results from laboratory experiments that were able to show an efficacy of ozone against SARS-CoV-2 [ [32] , [33] , [34] ].…”

Section: Discussionsupporting

confidence: 58%

An automated room disinfection system using ozone is highly active against surrogates for SARS-CoV-2

Franke¹,

Knobling²,

Brill³

et al. 2021

Journal of Hospital Infection

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Background The presence of coronaviruses on surfaces in the patient environment is a potential source of indirect transmission. Manual cleaning and disinfection measures do not always achieve sufficient removal of surface contamination. This increases the importance of automated solutions in the context of final disinfection of rooms in the hospital setting. Ozone is a highly effective disinfectant which, combined with high humidity, is an effective agent against respiratory viruses. Current devices allow continuous nebulization for high room humidity as well as ozone production without any consumables. Aim In the following study, the effectiveness of a fully automatic room decontamination system based on ozone was tested against bacteriophage Φ6 (phi 6) and bovine coronavirus L9, as surrogate viruses for the pandemic coronavirus SARS-CoV-2. Methods For this purpose, various surfaces (ceramic tile, stainless steel surface and furniture board) were soiled with the surrogate viruses and placed at two different levels in a gas-tight test room. After using the automatic decontamination device according to the manufacturer's instructions, the surrogate viruses were recovered from the surfaces and examined by quantitative cultures. Then, reduction factors were calculated. Findings The ozone-based room decontamination device achieved virucidal efficacy (reduction factor >4 log10) against both surrogate organisms regardless of the different surfaces and positions confirming a high activity under the used conditions. Conclusion Ozone is highly active against SARS-CoV-2 surrogate organisms. Further investigations are necessary for a safe application and efficacy in practice as well as integration into routine processes.

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Section: Discussionsupporting

confidence: 58%

An automated room disinfection system using ozone is highly active against surrogates for SARS-CoV-2

Franke¹,

Knobling²,

Brill³

et al. 2021

Journal of Hospital Infection

View full text Add to dashboard Cite

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“…60-65 m ( Dave et al, 2020 ) Poliovirus PV Picornaviridae NO 2 enteric virus, causative of polio ssRNA (+) 6.7–10.1 kB 30–32 nm icosahedral virions comprising 60 protomers ( Bolton et al, 1982 , Hudson et al, 2009 ) PR772 PR772 Tectiviridae NO 1 phage virus, model for adenovirus linear dsDNA, 15 kb icosahedral virion with a pseudo T=25 symmetry.66-80 nm with apical spikes of 20 nm. ( Dubuis et al, 2020 ) Reo type 3 virus RT3 reoviridae NO 1 dsRNA virus, 18.2-30.5 kB icosahedral virion, multilayer capsid structure, 60-80 nm ( Sato et al, 1990 ) Respiratory syncytial virus A2 RSV Pneumoviridae YES 1 human pathogen ssRNA (-) 13-15 kB spherical and filamentous virions, 150 nm ( Blanchard et al, 2020 ) Rhinovirus types 1 A & 14 RV Picornaviridae NO 1 Common cold virus, enterovirus ssRNA (+) 6.7–10.1 kB 30–32 nm icosahedral virions comprising 60 protomers ( Hudson et al, 2009 ) SARS-CoV-2 coronaviridiae YES 2 COVID19 responsible ssRNA (+), 29.9 kB spherical, some pleomorphism, 60-140 nm ( Clavo et al, 2020 ), ( Yano et al, 2020 ) Sendai virus HVJ Paramyxoviridae yes 1 representative respirovirus ssRNA (-) virus 15 kb pleomorphic, but usually spherical or filamentous, >150 nm ( …”

Section: Methodsmentioning

confidence: 99%

“…Regarding the virus experimentally tested and gathered in Table 3 , it is worth noting that at the time this review was written there were already two publications in which ozone was tested against the responsible agent for current pandemic, that is, SARS-CoV-2 and it was proven effective ( Clavo et al, 2020 , Yano et al, 2020 ). Furthermore, there were also three works ( Blanchard et al, 2020 , Hudson et al, 2009 , Lee et al, 2020 ) which proposed 4 different surrogates for this species (influenza A virus, respiratory syncytial virus, murine coronavirus and HCoV-229E) against which ozone was again operative.…”

Section: Virus Inactivation By Ozonementioning

confidence: 99%

“…2 , Fig. 3 show CT value versus the virus inactivation in aerosols (from 4 publications ( de Mik and de Groot, 1977 ; Dubuis et al, 2020 ; Kekez and Sattar, 1997 ; Tseng and Li, 2006 )) and on surfaces (from 10 publications: ( Blanchard et al, 2020 , Cannon et al, 2013 , Dave et al, 2020 , Hudson et al, 2009 , Lee et al, 2020 , Predmore et al, 2015 , Tanaka et al, 2009 , Tseng and Li, 2008 , Yano et al, 2020 ) according to full data in Tables S1 and S2 respectively. Only those experiments with a RH between 65%-95% (near optimal conditions) and inactivation quantification by plaque forming units (PFU) assay or the tissue culture infective dose have been included.…”

Section: Virus Inactivation By Ozonementioning

confidence: 99%

“…At the time this publication was submitted, only two very recent, concise works were found in literature where O 3 was applied for the disinfection of SARS-CoV-2 ( Clavo et al, 2020 , Yano et al, 2020 ). Also, a paper reviewing the use of ozone for indoor air treatment against viruses was published ( Alimohammadi and Naderi, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Can ozone inactivate SARS-CoV-2? A review of mechanisms and performance on viruses

Bayarri

Cruz-Alcalde

López-Vinent

et al. 2021

Journal of Hazardous Materials

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Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic has challenged societies around the globe. Technologies based on ozone, a powerful oxidant, have been evaluated to inactivate this virus in aerosols and fomites. However, the high data diversity hinders the possibility of establishing a common ground for determining best practices for the use of these technologies. Furthermore, there is a lack of consensus regarding which are the main mechanisms of ozone virus inactivation. This critical review examined the most relevant information available regarding ozone application in gas-phase for different viruses inactivation (including recent publications dealing with SARS-CoV-2), and pointed towards envelope alteration as the main reaction pathway for enveloped viruses, such as is the case of SARS-CoV-2. It could also be concluded that gaseous ozone can be indeed an effective disinfectant, successfully inactivating viruses such us influenza A H1N1, MERS-CoV, SARS-CoV-1 or even SARS-CoV-2 in aerosols or fomites. In reviewed works, low ozone exposures, just around 0.1-0.4 mg L -1 min, achieve about 4 log 10 of inactivation in aerosols, while exposures between 1 and 4 mg L -1 min may be needed to guarantee an inactivation of 3-4 log 10 in different fomites. Although further studies are required, ozone is an effective candidate to be used against SARS-CoV-2 or other viruses in surfaces and indoor locations.

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Inactivation of Pathogenic Microorganisms on Polymeric Materials through Ozonation

et al. 2023

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Ozone-based technologies have been evaluated to inactivate the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on surfaces. However, the vast diversity of information makes it difficult to establish common ground for determining the best practices for using this technology. The objective of this work is to evaluate the success of N95 mask decontamination by ozonation, determining the specific parameters for process control. To quantify the effectiveness of the process, a disinfection protocol was initially developed based on two bacterial species (Escherichia coli and Staphylococcus pseudintermedius), followed by another disinfection assay using the murine hepatitis coronavirus (MHV-3), in a laboratory-scale prototype. Ozone is an effective candidate for use against SARS-CoV-2 or other viruses to disinfect personal protection equipment (PPE).

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Inactivation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by gaseous ozone treatment

Cited by 53 publications

References 7 publications

An automated room disinfection system using ozone is highly active against surrogates for SARS-CoV-2

An automated room disinfection system using ozone is highly active against surrogates for SARS-CoV-2

Can ozone inactivate SARS-CoV-2? A review of mechanisms and performance on viruses

Inactivation of Pathogenic Microorganisms on Polymeric Materials through Ozonation

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