Inactivation of viruses in labile blood derivatives. I. Disruption of lipid‐enveloped viruses by tri(n‐butyl)phosphate detergent combinations

Horowitz, Bernard; Wiebe, Michael E.; Lippin, Arnold; Stryker, Martin H.

doi:10.1046/j.1537-2995.1985.25686071422.x

Cited by 333 publications

(156 citation statements)

References 16 publications

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“…FCV, a non-enveloped virus, has a capsid that consists of a protein and lacks an envelope that surrounds it. Although enveloped viruses are effectively eliminated by solvent/detergent treatment, non-enveloped viruses are not affected by this treatment [44]. Non-enveloped viruses, without a lipid coat, are resistant to most of the viral inactivation methods that have been described to date [45,46].…”

Section: Virucidal Activity Of Photocatalysis By Tio 2 -Coated Glassmentioning

confidence: 99%

Broad Spectrum Microbicidal Activity of Photocatalysis by TiO2

et al. 2013

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Photocatalytically active titanium dioxide (TiO 2 ) is widely used as a self-cleaning and self-disinfecting material in many applications to keep environments biologically clean. Several studies on the inactivation of bacteria and viruses by photocatalytic reactions have also been reported; however, only few studies evaluated the spectrum of the microbicidal activity with photocatalysis for various species. There is a need to confirm the expected OPEN ACCESSCatalysts 2013, 3 311 effectiveness of disinfection by photocatalysis against multidrug-resistant bacteria and viruses. In this study, microbicidal activity of photocatalysis was evaluated by comparing the inactivation of various species of bacteria and viruses when their suspensions were dropped on the surface of TiO 2 -coated glass. Gram-positive bacteria, e.g., methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecalis, and penicillin-resistant Streptococcus pneumoniae, were easily inactivated by photocatalysis, whereas some gram-negative bacteria, e.g., Escherichia coli and multidrug-resistant Pseudomonas aeruginosa, were gradually inactivated by photocatalysis. Influenza virus, an enveloped virus, was significantly inactivated by photocatalysis compared with feline calicivirus, a non-enveloped virus. The effectiveness of microbicidal activity by photocatalysis may depend on the surface structure. However, they are effectively inactivated by photocatalysis on the surface of TiO 2 -coated glass. Our data emphasize that effective cleaning and disinfection by photocatalysis in nosocomial settings prevents pathogen transmission.

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Section: Virucidal Activity Of Photocatalysis By Tio 2 -Coated Glassmentioning

confidence: 99%

Broad Spectrum Microbicidal Activity of Photocatalysis by TiO2

et al. 2013

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“…From the mid-1980s to the early 1990s, heat treatment of freeze-dried coagulation factors at 60-68°C for 24-96 h or 80°C for 72 h were developed to inactivate HIV and HCV concomitantly [156]. Although pasteurization has successfully been adapted to several plasma products (such as antithrombin and alpha 1-antitrypsin), a milestone in the safety of industrial plasma products was the development of the solvent/detergent (S/D) incubation procedure at 20-37°C [157] designed to dissolve the lipid envelope of viruses, including HCV, without affecting plasma protein functions. This technique is still largely used for a wide range of industrial plasma products owing to wellproven efficacy and a safety profile established by years of industrial and clinical practices [158].…”

Section: Hcv Reduction Treatment Of Industrial Plasma Protein Productsmentioning

confidence: 99%

Strategies to Preclude Hepatitis C Virus Entry

Burnouf¹,

Lin²

2017

Advances in Treatment of Hepatitis C and B

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Without a preventive vaccine, hepatitis C virus (HCV) remains an important pathogen worldwide with millions of carriers at risk of end-stage liver diseases. Despite the introduction of novel direct-acting antivirals (DAAs), resistance problems, challenges with the difficult-to-treat populations and high costs limit the widespread application of these drugs. Antivirals with alternative mechanism(s) of action, such as by restricting viral entry or cell-to-cell spread, could help expand the scope of antiviral strategies for the management of hepatitis C. Transfusion-associated HCV infection remains another issue in endemic and resource-limited areas around the world. This chapter describes some of the latest developments in antiviral strategies to preclude HCV entry, such as through monoclonal antibodies and small molecules, as well as measures to enhance the safety of therapeutic plasma products in blood transfusion.

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“…A 30 kD Millipore Pellicon Systen was used to perform diafiltration and protein level adjustment. Virus inactivation was performed using a solvent (tri-n-butyl phosphate; TNBP) and a detergent (Tween 80) (Horowitz et al, 1985). To remove the solvent and detergent, a cation-exchange chromatography using a CM Sepharose FF stationary phase was performed.…”

Section: Immunoglobulin Fractionationmentioning

confidence: 99%

Production of intravenous human dengue immunoglobulin from Brazilian-blood donors

Gouveia

Oliveira

Lucena³

et al. 2013

Braz. J. Pharm. Sci.

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Dengue represents an important health problem in Brazil and therefore there is a great need to develop a vaccine or treatment. The neutralization of the dengue virus by a specific antibody can potentially be applied to therapy. The present paper describes, for the first time, the preparation of Immunoglobulin specific for the dengue virus (anti-DENV IgG), collected from screened Brazilian blood-donations. Production was performed using the classic Cohn-Oncley process with minor modifications. The anti-DENV IgG was biochemically and biophysically characterized and fulfilled the requirements defined by the European Pharmacopoeia. The finished product was able to neutralize different virus serotypes (DENV-1, DENV-2, and DENV-3), while a commercial IgG collected from American blood donations was found to have low anti-dengue antibody titers. Overall, this anti-DENV IgG represents an important step in the study of the therapeutic potential and safety of a specific antibody that neutralizes the dengue virus in humans.Uniterms:. Viral infections. Dengue/treatment. Dengue/virus/neutralization. Immunoglobulins/ preparation. Plasma fractionation. Virus neutralization.A dengue representa um importante problema de saúde no Brasil, portanto, a identificação de vacina ou tratamento eficaz é uma necessidade urgente. A neutralização do vírus da dengue, por anticorpo específico, tem potencial aplicação terapêutica. Descrevemos aqui, pela primeira vez, a preparação de imunoglobulina específica para o vírus da dengue (IgG anti-DENV), produzida a partir do plasma selecionado de doadores brasileiros. A produção foi realizada utilizando o método clássico de Cohn-Oncley com pequenas modificações. A IgG anti-DENV foi bioquimicamente e biofisicamente caracterizada e cumpriu os requisitos definidos pela Farmacopeia Europeia. O produto final foi capaz de neutralizar diferentes sorotipos do vírus (DENV-1, DENV-2 e DENV-3), enquanto que a IgG comercial, produzida a partir do plasma de doadores americanos, apresentou baixos títulos de anticorpos anti-dengue. No geral, esta IgG anti-DENV representa uma importante etapa para o estudo do potencial terapêutico e segurança da neutralização, por anticorpos específicos, do vírus da dengue em humanos.Unitermos: Infecções virais. Dengue/tratamento. Dengue/vírus/neutralização. Imunoglobulinas específicas/preparação. Fracionamento de plasma. Neutralização viral. INTRODUCTIONThere are around 2.5 billion people living in areas endemic for dengue fever and it is estimated that there are 100 million infections annually (Gubler 1998). The burden of dengue in endemic countries weighs heaviest on children. However, there has been an increasing trend towards adult infection in certain countries (Ooi, Goh, Gubler, 2006;Cummings et al., 2009). Most travelers with dengue have been adults (Wilder-Smith, Schwartz, 2005). Currently, neither cure nor vaccines are available, and new therapies are thus urgently needed. Although there have been considerable developments in medicinal chemistry, no promising small-organic...

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Inactivation of viruses in labile blood derivatives. I. Disruption of lipid‐enveloped viruses by tri(n‐butyl)phosphate detergent combinations

Cited by 333 publications

References 16 publications

Broad Spectrum Microbicidal Activity of Photocatalysis by TiO2

Broad Spectrum Microbicidal Activity of Photocatalysis by TiO2

Strategies to Preclude Hepatitis C Virus Entry

Production of intravenous human dengue immunoglobulin from Brazilian-blood donors

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