IncF plasmid diversity in multi-drug resistant Escherichia coli strains from animals in China

Qin, Yang; Sun, Jian; Li, Liang; Deng, Hui; Liu, Bao Tao; Fang, Liang Xing; Liao, Xiaoping; Liu, Ya Hong

doi:10.3389/fmicb.2015.00964

Cited by 97 publications

(72 citation statements)

References 52 publications

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“…The two isolates although not clonally related, shared the same plasmids (Col440I) harbouring AMR genes (qnrB19) due to horizontal gene transfer. Studies have shown that the IncF and IncHI2 plasmids mainly found in E. coli strains, are frequently detected in humans and animals serving as reservoirs for the spread of AMR genes and have been associated with MDR E. coli [43,59]. This evidence supports our study results and explanation of a possibility of horizontal gene transfer of AMR genes harboured in the plasmids.…”

Section: Discussionsupporting

confidence: 91%

Genetic relatedness of multidrug resistant Escherichia coli isolated from humans, chickens and poultry environments

Aworh

Kwaga

Hendriksen

et al. 2020

Preprint

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Background: Inappropriate use of antimicrobial agents in animal production has led to the development of antimicrobial resistance (AMR) in foodborne pathogens. Transmission of AMR foodborne pathogens from reservoirs, particularly chickens to the human population do occur. Recently, we reported that occupational exposure was a risk factor for multidrug-resistant (MDR) Escherichia coli (E. coli) among poultry-workers. Here we determined the prevalence and genetic relatedness among MDR E. coli isolated from poultry-workers, chickens, and poultry environments in Abuja, Nigeria. This study was conducted to address the gaps identified by the Nigerian AMR situation analysis.Methods: We conducted a cross-sectional study among poultry-workers, chickens, and poultry farm/live bird market (LBM) environments. The isolates were tested phenotypically for their antimicrobial susceptibility profiles, genotypically characterized using whole-genome sequencing (WGS) and in silico multilocus sequence types (MLST). We conducted a phylogenetic single nucleotide polymorphism (SNPs) analysis to determine relatedness and clonality among the isolates.Results: A total of 115 (26.8%) out of 429 samples were positive for E. coli. Of these, 110 isolates were viable for phenotypic and genotypic characterization. The selection comprised 47 (42.7%) isolates from poultry-workers, 36 (32.7%) from chickens, and 27 (24.5%) from poultry-farm or LBM environments. Overall, 101 (91.8%) isolates were determined MDR conferring resistance to at least three drug classes. High frequency of resistance were observed for tetracycline (n = 102; 92.7%), trimethoprim/sulfamethoxazole (n = 93; 84.5%), streptomycin (n = 87; 79.1%) and ampicillin (n = 88; 80%). Two plasmid-mediated colistin genes – mcr-1.1 harboured on IncX4 plasmids were detected in environmental isolates. The most prevalent sequence types (ST) were ST-155 (n = 8), ST-48 (n = 8) and ST-10 (n = 6). Two isolates of human and environmental sources with a SNPs difference of 6161 originating from the same farm shared a novel ST. The isolates had similar AMR genes and plasmid replicons.Conclusion: MDR E.coli isolates were prevalent amongst poultry-workers, poultry, and the poultry farm/LBM environment. The emergence of MDR E. coli with novel ST in two isolates may be plasmid-mediated. Competent authorities should enforce AMR regulations to ensure prudent use of antimicrobials to limit the risk of transmission along the food chain.

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Section: Discussionsupporting

confidence: 91%

Genetic relatedness of multidrug resistant Escherichia coli isolated from humans, chickens and poultry environments

Aworh

Kwaga

Hendriksen

et al. 2020

Preprint

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“…The diversity of plasmid types was increased in comparison with those in previous report (Tamang et al, 2013b), which may reflect more influx of various antimicrobial-resistant genes. Among IncF plasmids in the bla CTX-M-55 -positive E. coli isolates from animals in China, F33 plasmids were the most prevalent replicon STs (Yang et al, 2015). In contrast, F1 types, which are commonly found to carry ESBL genes (Brolund, 2014), were most predominantly identified in this study.…”

Section: Discussionmentioning

confidence: 53%

Clinically Relevant Extended-Spectrum β-Lactamase–Producing Escherichia coli Isolates From Food Animals in South Korea

Song

Kim

et al. 2020

Front. Microbiol.

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Extended-spectrum β-lactam antimicrobials have been broadly used in food animals and humans to control infectious diseases. However, the emergence and rapid spread of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, mainly Escherichia coli, have seriously threatened global health in recent decades. In this study, we determined the prevalence, antimicrobial susceptibility, and genetic properties of ESBL-producing E. coli (ESBL-EC) strains isolated from food animals in South Korea. A total of 150 fecal samples from healthy chickens (n = 34), pigs (n = 59), and cattle (n = 57) were screened from January to July 2018. Among these, 77 non-duplicate cefotaxime-resistant ESBL-EC strains were isolated from 32 chicken, 41 pig, and 4 cattle samples, with the corresponding occurrence rates of 94.1, 69.5, and 7.0%, respectively. All the isolates showed multidrug resistance (MDR) and produced at least one type of β-lactamase, including CTX-M (98.7%) and TEM (40.3%). CTX-M-14 (53.1%), CTX-M-55 (53.7%), and CTX-M-65 (50.0%) were the predominant genotypes in the chicken, pig, and cattle samples, respectively. Multilocus sequence typing revealed 46 different sequence types (STs), including the human-associated extraintestinal pathogenic E. coli ST131 (n = 2), ST10 (n = 5), ST38 (n = 1), ST410 (n = 4), ST354 (n = 2), ST58 (n = 3), ST117 (n = 1), and ST457 (n = 1). To the best of our knowledge, this is the first report of pandemic E. coli ST131 in non-human isolates in South Korea. Our results demonstrate the high prevalence and diversity of MDR-ESBL-EC in food animals and highlight them as potential pathogenic ESBL-EC reservoirs that may pose a high risk to human health.

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“…Results from the PlasmidFinder pipeline indicated that the isolated E. coli strains carried an IncF plasmid (replicon type II-IA-IB). The conjugative plasmids of the IncF family range in size from 45 to 200 kb [16, 51,52]. They seem to have a narrow host range specific to the Enterobacteriaceae, being mostly found in E. coli.…”

Section: Overview Of the Draft Genomes Of The Esbl-producing Enterobamentioning

confidence: 99%

“…They seem to have a narrow host range specific to the Enterobacteriaceae, being mostly found in E. coli. Furthermore, they are the predominant plasmids involved in the dissemination of bla CTX-M-15 and are widely reported in bacteria isolated from humans, animals and the environment [52]. IncFII-IA-IB plasmids carrying bla CTX-M have been implicated in community-associated infections on several continents, emphasizing the epidemic nature and global distribution of these plasmids [53,54].…”

Section: Overview Of the Draft Genomes Of The Esbl-producing Enterobamentioning

confidence: 99%

A survey of extended-spectrum beta-lactamase-producing Enterobacteriaceae in urban wetlands in southwestern Nigeria as a step towards generating prevalence maps of antimicrobial resistance

et al. 2020

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In many countries, emission of insufficiently treated wastewater into water bodies appears to be an important factor in spreading clinically relevant antimicrobial resistant bacteria. In this study, we looked for the presence of Enterobacteriaceae strains with resistance to 3 rd generation cephalosporin antibiotics in four urban wetlands in southwestern Nigeria by isolation, whole genome sequencing and qPCR enumeration of marker genes. Genome analysis of multi-drug resistant and potentially pathogenic Escherichia coli isolates (members of the widely distributed ST10 complex) revealed the presence of the extended spectrum beta-lactamase gene bla CTX-M-15 on self-transmissible IncF plasmids. The gene was also present together with a bla TEM-1B gene on self-transmissible IncH plasmids in multi-drug resistant Enterobacter cloacae isolates. A Citrobacter freundii isolate carried bla TEM-1B on an IncRtype plasmid without discernable conjugation apparatus. All strains were isolated from a wetland for which previous qPCR enumeration of marker genes, in particular the ratio of intI1 to 16S rRNA gene copy numbers, had indicated a strong anthropogenic impact. Consistent with the isolation origin, qPCR analysis in this study showed that the bla CTX-M gene was present at an abundance of 1x10 -4 relative to bacterial 16S rRNA gene copy numbers.The results indicate that contamination of these urban aquatic ecosystems with clinically relevant antibiotic resistant bacteria is substantial in some areas. Measures should therefore be put in place to mitigate the propagation of clinically relevant antimicrobial resistance within the Nigerian aquatic ecosystems. PLOS ONEPLOS ONE | https://doi.org/10.

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IncF plasmid diversity in multi-drug resistant Escherichia coli strains from animals in China

Cited by 97 publications

References 52 publications

Genetic relatedness of multidrug resistant Escherichia coli isolated from humans, chickens and poultry environments

Genetic relatedness of multidrug resistant Escherichia coli isolated from humans, chickens and poultry environments

Clinically Relevant Extended-Spectrum β-Lactamase–Producing Escherichia coli Isolates From Food Animals in South Korea

A survey of extended-spectrum beta-lactamase-producing Enterobacteriaceae in urban wetlands in southwestern Nigeria as a step towards generating prevalence maps of antimicrobial resistance

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