IncF Plasmids Are Commonly Carried by Antibiotic Resistant<i>Escherichia coli</i>Isolated from Drinking Water Sources in Northern Tanzania

Lyimo, Beatus; Buza, Joram; Subbiah, Murugan; Temba, Sylivester; Kipasika, H. J; Smith, Woutrina A.; Call, Douglas R.

doi:10.1155/2016/3103672

Cited by 54 publications

(27 citation statements)

References 40 publications

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“…The dissemination of ESBL genes is reportedly mediated by the IncI1 plasmid [21]. E. coli was also reported to maintain the IncI1 plasmid without antibiotic selection pressure [22]. Thus, our findings suggested that the IncI1 plasmid has an important role in the spread of bla CTX-M-2 in racehorses.…”

Section: Discussionsupporting

confidence: 53%

IncI1 Plasmid Associated with blaCTX-M-2 Transmission in ESBL-Producing Escherichia coli Isolated from Healthy Thoroughbred Racehorse, Japan

et al. 2020

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In our previous study, extended spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBLEC) were isolated from healthy Thoroughbred racehorse feces samples in Japan. Some ESBL genes were predicted to be located on the conjugative plasmid. PCR-based replicon typing (PBRT) is a useful method to monitor and detect the association of replicons with specific plasmid-borne resistant genes. This study aimed to evaluate the plasmid replicon associated with ESBLEC isolated from healthy Thoroughbred racehorses at Japan Racing Association Training Centers in Japan. A total of 24 ESBLECs isolated from 23 (10.8%) individual Thoroughbred racehorse feces samples were used in this study. ESBL gene transfer was performed using a conjugation assay. Then, replicon types of ESBLEC isolates and their transconjugants were determined using PBRT. Pulsed-field gel electrophoresis (PFGE) was performed to look at the clonality of the ESBLECs isolates. ESBLECs were detected from 10.8% of healthy Thoroughbred racehorses. The blaCTX-M-2 was identified as the dominant type of ESBL gene, followed by blaCTX-M-1 and blaTEM-116. In this study, only the blaCTX-M-2 and the IncI1 plasmid were transferred to transconjugants. The PFGE results showed that ESBL genes were distributed in diversity of ESBLECs. This finding suggested that the IncI1 plasmid was associated with the dissemination of blaCTX-M-2 in Thoroughbred racehorses in Japan.

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Section: Discussionsupporting

confidence: 53%

IncI1 Plasmid Associated with blaCTX-M-2 Transmission in ESBL-Producing Escherichia coli Isolated from Healthy Thoroughbred Racehorse, Japan

et al. 2020

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“…The mating was done as described previously [ 25 ]. Donor strains were grown on LB (Luria-Bertani) plates containing antibiotics (as per donor’s antibiotic resistance listed in Table 2), from frozen stocks, by overnight incubation at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

American Crows as Carriers of Extra Intestinal Pathogenic E. coli and Avian Pathogenic-Like E. coli and Their Potential Impact on a Constructed Wetland

et al. 2020

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The study examines whether crows are carriers of extraintestinal pathogenic E. coli (ExPEC) and avian pathogenic E. coli (APEC)-like strains, and if wetland roost areas contribute to their spread. A total of 10 crow feces (n = 71) and 15 water E. coli isolates (n = 134) from a wetland area could be characterized as potentially ExPEC based on the presence of ≥2 of the five cardinal genes iutA, kpsMT2, papEF, pap A/C, papG, sfa/foc, and afa/dra, while six fecal and 14 water isolates could be characterized as potentially APEC-like based on the presence of plasmid associated genes: iutA, episomal iss, ompT, hlyF and iroN. A total of 32 fecal and 27 water isolates tested carried plasmids based on incompatibility typing. Plasmids from 34 of 38 isolates tested could be transferred to another E. coli strain by conjugation with the antibiotic resistance (AR) profile being transferred, indicating their potential to be transferred to indigenous and non-pathogenic strains in the wetland. APEC-like plasmids could be transferred in six of eight isolates tested. Pathogenic E. coli of importance to the medical community and poultry industry may be detected in high levels in surface water due to corvid activity. Regardless of their role in health or disease, water in wetlands and streams can serve as a media for the dissemination of AR and virulence traits of bacteria, with corvids acting as potential vectors for farther dissemination.

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“…The procedure was carried as described by Robinson and Batt (1999) and Lyimo et al (2016). Briefly, 10ml of milk sample was diluted into 90ml of double distilled sterile water.…”

Section: Isolation Of E Colimentioning

confidence: 99%

Prevalence and Antibiotic Susceptibility of Escherichia coli and Salmonella spp. isolated from milk of zero grazed cows in Arusha City

Sudda¹,

Mtenga²,

Kusiluka³

et al. 2016

Afr. J. Microbiol. Res.

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The present study assessed the antibiotic susceptibility patterns of Escherichia coli and Salmonella isolates of raw milk from zero grazed cows. A total of 65 milk samples were collected for analysis. The standard membrane filtration technique and HiCrome E. coli agar were used in isolation of E. coli from milk samples. Isolation of Salmonella species employed pre-enrichment in buffered peptone water followed by enrichment in Rappaport and Vassilidis broth prior to Xylose lysine deoxychocolate agar as a differential media. The isolates were analyzed for antimicrobial susceptibility to eight different types of antibiotics using disc diffusion method. The prevalence of E. coli was 16 (16.7%) and all the samples tested were negative for Salmonella. The average colony forming unit for E. coli was 2cfu/mL. All E. coli isolates tested were resistant to penicillin (100%) and amoxicillin-clavulanic acid (100%) while 15(93.8%) were sensitive to ciprofloxacin. Resistance was also observed in sulfamethoxazole-trimethoprim (43.8%), chloramphenicol (12.5%), oxytetracycline (68.8%), streptomycin (12.5%) and gentamicin (25%). Of the isolates tested, 14 (87.5%) showed multi-drug resistance pattern. These results confirm that milk from zero grazed cows in Arusha was contaminated with E. coli, and that most of the E. coli strains isolated were resistant to at least one of the antimicrobial agent commonly used in treatment of human diseases.

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IncF Plasmids Are Commonly Carried by Antibiotic ResistantEscherichia coliIsolated from Drinking Water Sources in Northern Tanzania

Cited by 54 publications

References 40 publications

IncI1 Plasmid Associated with blaCTX-M-2 Transmission in ESBL-Producing Escherichia coli Isolated from Healthy Thoroughbred Racehorse, Japan

IncI1 Plasmid Associated with blaCTX-M-2 Transmission in ESBL-Producing Escherichia coli Isolated from Healthy Thoroughbred Racehorse, Japan

American Crows as Carriers of Extra Intestinal Pathogenic E. coli and Avian Pathogenic-Like E. coli and Their Potential Impact on a Constructed Wetland

Prevalence and Antibiotic Susceptibility of Escherichia coli and Salmonella spp. isolated from milk of zero grazed cows in Arusha City

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